| Basic Information | |
|---|---|
| Species | Thellungiella halophila |
| Cazyme ID | Thhalv10008154m |
| Family | GT8 |
| Protein Properties | Length: 334 Molecular Weight: 38439 Isoelectric Point: 5.4292 |
| Chromosome | Chromosome/Scaffold: 5 Start: 12553820 End: 12555653 |
| Description | galactinol synthase 3 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 13 | 259 | 0 |
| RAYVTFLAGNGDYVKGVVGLAKGLRKSKSKYPLVVAVLPDVPVDHQRQLLEQGCVVKEIQPVYPPENQTQFAMAYYVLNYSKLRIWKLVEYSKMIYLDGD IQVFDNIDHLFDLPDGNFYAVKDCFCEKTWSHTPQYKIGYCQQCPGKVTWPEPELGPKPSLYFNAGMFVYEPSLSTYYNLLETLKVVSPTPFAEQDFLNM YFKDIYKPIPPIYNIVLAMLWRHPENIELDEAKVVHYCAAGSKPWRF | |||
| Full Sequence |
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| Protein Sequence Length: 334 Download |
| MTPEINSYGG KKRAYVTFLA GNGDYVKGVV GLAKGLRKSK SKYPLVVAVL PDVPVDHQRQ 60 LLEQGCVVKE IQPVYPPENQ TQFAMAYYVL NYSKLRIWKL VEYSKMIYLD GDIQVFDNID 120 HLFDLPDGNF YAVKDCFCEK TWSHTPQYKI GYCQQCPGKV TWPEPELGPK PSLYFNAGMF 180 VYEPSLSTYY NLLETLKVVS PTPFAEQDFL NMYFKDIYKP IPPIYNIVLA MLWRHPENIE 240 LDEAKVVHYC AAGSKPWRFT GQEENMERED IKILVEKWWD IYNDESLDYK NINVHCGQEE 300 EDVHRKHQTT LPQFLTDLSE AVVLQCAKAP SAA* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1442 | RfaJ | 7.0e-13 | 92 | 288 | 212 | + Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases [Cell envelope biogenesis, outer membrane] | ||
| cd04194 | GT8_A4GalT_like | 5.0e-16 | 102 | 259 | 181 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| pfam01501 | Glyco_transf_8 | 3.0e-56 | 16 | 261 | 264 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 1.0e-77 | 13 | 285 | 276 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| PLN00176 | PLN00176 | 0 | 1 | 333 | 343 | + galactinol synthase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM19710.1 | 0 | 9 | 333 | 20 | 337 | AF499723_1 galactinol synthase-like protein [Thellungiella halophila] |
| EMBL | CAN79630.1 | 0 | 1 | 333 | 1 | 342 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_172406.1 | 0 | 1 | 333 | 1 | 334 | AtGolS3 (Arabidopsis thaliana galactinol synthase 3); transferase, transferring glycosyl groups / transferase, transferring hexosyl groups |
| RefSeq | NP_176053.1 | 0 | 1 | 333 | 1 | 335 | AtGolS2 (Arabidopsis thaliana galactinol synthase 2); transferase, transferring glycosyl groups / transferase, transferring hexosyl groups |
| RefSeq | XP_002320958.1 | 0 | 12 | 333 | 23 | 336 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2x_B | 5e-20 | 13 | 287 | 5 | 259 | A Chain A, High Resolution Crystal Structure Of A Thermostable Xylanase From Thermoascus Aurantiacus |
| PDB | 3u2x_A | 5e-20 | 13 | 287 | 5 | 259 | A Chain A, High Resolution Crystal Structure Of A Thermostable Xylanase From Thermoascus Aurantiacus |
| PDB | 3u2v_B | 5e-20 | 13 | 287 | 5 | 259 | A Chain A, High Resolution Crystal Structure Of A Thermostable Xylanase From Thermoascus Aurantiacus |
| PDB | 3u2v_A | 5e-20 | 13 | 287 | 5 | 259 | A Chain A, High Resolution Crystal Structure Of A Thermostable Xylanase From Thermoascus Aurantiacus |
| PDB | 3u2u_B | 5e-20 | 13 | 287 | 5 | 259 | A Chain A, High Resolution Crystal Structure Of A Thermostable Xylanase From Thermoascus Aurantiacus |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DT564633 | 280 | 12 | 291 | 0 |
| CV198253 | 279 | 13 | 291 | 0 |
| EX136542 | 326 | 8 | 333 | 0 |
| CK278474 | 282 | 13 | 294 | 0 |
| EX136535 | 283 | 8 | 290 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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