y
Basic Information | |
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Species | Thellungiella halophila |
Cazyme ID | Thhalv10010231m |
Family | GH32 |
Protein Properties | Length: 590 Molecular Weight: 66734 Isoelectric Point: 9.8169 |
Chromosome | Chromosome/Scaffold: 16 Start: 1465775 End: 1468366 |
Description | cell wall invertase 2 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 55 | 370 | 0 |
HFQPPRHWINGPMIYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGITENQTQIQNYAIPQ DLSDPYLKKWIKPDDNPIVRPNHGENGSAFRDPTTAWFNKKDGYWRMLVGSKRKHRGIAYMYKSRDFKKWVKTKRPIHSRKKTGMWECPDFFPVSTDKKT GLDFSYEGPNTKHVLKVSLDLTRYEYYTLGKYDTKKDRYRPDGNTPDGWDGLRFDYGNFYASKTFFDDMKNRRILWGWANESDTVEDDTSKGWAGIQLIP RTVLLDSSGKQLVFWP |
Full Sequence |
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Protein Sequence Length: 590 Download |
MSASKIVSVL VLFVLINISN NNINGVDAFH KVYRKLQSKS TSLEIVSPLH RTAFHFQPPR 60 HWINGPMIYK GVYHLFYQYN PKGAVWGNIV WAHSVSKDLI NWEALEPAIY PSKWFDINGT 120 WSGSATHVPG KGPVILYTGI TENQTQIQNY AIPQDLSDPY LKKWIKPDDN PIVRPNHGEN 180 GSAFRDPTTA WFNKKDGYWR MLVGSKRKHR GIAYMYKSRD FKKWVKTKRP IHSRKKTGMW 240 ECPDFFPVST DKKTGLDFSY EGPNTKHVLK VSLDLTRYEY YTLGKYDTKK DRYRPDGNTP 300 DGWDGLRFDY GNFYASKTFF DDMKNRRILW GWANESDTVE DDTSKGWAGI QLIPRTVLLD 360 SSGKQLVFWP IEEIESLRGK NVQMNNQKME KGQRFEVQGI TPAQVDVDVT FNVGSLEKAE 420 NFDPSFATKP LDLCNLKGSN VTGGVGPFGL ITLATSDLEE YTPVFFRVFK DATTNKPKVL 480 MCSDAKPSTL KTDTGSDAKE KMYKPSFAGF VDVDLADGKI SLRSLIDHSV VESFGAKGKT 540 VITSRVYPTK AVGDKAHLFV FNNGSQPVTV ESLNAWNMQK PLKMNQGAK* 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 6.0e-49 | 50 | 548 | 523 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 3.0e-58 | 50 | 549 | 522 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-86 | 61 | 373 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-142 | 55 | 370 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-175 | 55 | 538 | 496 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAA63802.1 | 0 | 1 | 589 | 1 | 590 | invertase [Arabidopsis thaliana] |
GenBank | ABK28599.1 | 0 | 1 | 589 | 1 | 590 | unknown [Arabidopsis thaliana] |
DDBJ | BAC42957.1 | 0 | 1 | 589 | 1 | 590 | putative beta-fructofuranosidase [Arabidopsis thaliana] |
EMBL | CAB43403.1 | 0 | 1 | 589 | 1 | 587 | beta-fructofuranosidase [Arabidopsis thaliana] |
RefSeq | NP_190828.2 | 0 | 1 | 589 | 1 | 590 | AtcwINV2 (Arabidopsis thaliana cell wall invertase 2); hydrolase, hydrolyzing O-glycosyl compounds |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2qqw_A | 0 | 50 | 578 | 4 | 531 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
PDB | 2ac1_A | 0 | 50 | 578 | 8 | 535 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
PDB | 2xqr_K | 0 | 50 | 578 | 4 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_I | 0 | 50 | 578 | 4 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_G | 0 | 50 | 578 | 4 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |