y
Basic Information | |
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Species | Thellungiella halophila |
Cazyme ID | Thhalv10013192m |
Family | GH32 |
Protein Properties | Length: 542 Molecular Weight: 61068.5 Isoelectric Point: 4.8311 |
Chromosome | Chromosome/Scaffold: 2 Start: 3909126 End: 3912164 |
Description | 6-&1-fructan exohydrolase |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 18 | 334 | 0 |
HFQPHRNWINAPMYYKGFYHMFYQHNPLAPEFNDIIIWGHSVSQDMVNWIQLEPALFPTEPFDRNSCWSGSATILPDGKPVIMYTGLHEPNRQQVTVIAE PKDVSDPLLREWVKPKYNPVMLPPDDIPFDGFRDPTTAWQGQDGKWRVLVGARQIDTERGMAILYHSDDFVQWTRHPVPLLASQDTGMWECPDFFPVSIT GKEGADTSVNNASVKHVLKSSFGGHDCYVIGTYSSETEEFAADSEFTNTSADLRYDHGTFYASKAFFDSVKNRRINWGWVIETDSKEDDFQKGWSGLLSL PREIWLDTSGKRLIQWP |
Full Sequence |
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Protein Sequence Length: 542 Download |
MAEHGNLQNL PLNRTSFHFQ PHRNWINAPM YYKGFYHMFY QHNPLAPEFN DIIIWGHSVS 60 QDMVNWIQLE PALFPTEPFD RNSCWSGSAT ILPDGKPVIM YTGLHEPNRQ QVTVIAEPKD 120 VSDPLLREWV KPKYNPVMLP PDDIPFDGFR DPTTAWQGQD GKWRVLVGAR QIDTERGMAI 180 LYHSDDFVQW TRHPVPLLAS QDTGMWECPD FFPVSITGKE GADTSVNNAS VKHVLKSSFG 240 GHDCYVIGTY SSETEEFAAD SEFTNTSADL RYDHGTFYAS KAFFDSVKNR RINWGWVIET 300 DSKEDDFQKG WSGLLSLPRE IWLDTSGKRL IQWPIEEINN LRTRQVTLDN KELKGSSILE 360 ISGITASQAD VEVTFDLPVL EENSQILDSD QVDDAVLFDR DSLVSCVYGP FGLLALASSD 420 LSEQTAIFFK IIRCGNGYSV IMGSDEKKSS LRDNIKKSTH GTVLDIDPRH EKISLRCLID 480 HSIIESYGAG GRNVITSRVY PNLAIGEAAK LYMFNDGTRS VTMSSLEAWS MRNAEVNSNA 540 I* 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-51 | 14 | 502 | 508 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-60 | 14 | 501 | 508 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 7.0e-86 | 24 | 337 | 321 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 3.0e-129 | 18 | 334 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 6.0e-155 | 18 | 491 | 487 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAB87665.1 | 0 | 1 | 539 | 4 | 544 | fructosidase-like protein [Arabidopsis thaliana] |
EMBL | CAD49079.1 | 0 | 14 | 536 | 46 | 568 | fructan 1-exohydrolase [Campanula rapunculoides] |
RefSeq | NP_001078574.1 | 0 | 1 | 403 | 4 | 411 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
RefSeq | NP_568254.1 | 0 | 1 | 539 | 4 | 547 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
RefSeq | XP_002309497.1 | 0 | 14 | 537 | 47 | 567 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 14 | 535 | 8 | 532 | A Chain A, Barley Limit Dextrinase In Complex With Beta-Cyclodextrin |
PDB | 2ade_A | 0 | 14 | 535 | 8 | 532 | A Chain A, Barley Limit Dextrinase In Complex With Beta-Cyclodextrin |
PDB | 2add_A | 0 | 14 | 535 | 8 | 532 | A Chain A, Barley Limit Dextrinase In Complex With Beta-Cyclodextrin |
PDB | 1st8_A | 0 | 14 | 535 | 8 | 532 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 14 | 535 | 8 | 532 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CT842376 | 538 | 11 | 537 | 0 |
FD956604 | 260 | 271 | 528 | 0 |
EE591202 | 415 | 132 | 538 | 0 |
EE591202 | 128 | 13 | 136 | 0 |
FD949297 | 214 | 1 | 208 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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