Basic Information | |
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Species | Thellungiella halophila |
Cazyme ID | Thhalv10016415m |
Family | GH32 |
Protein Properties | Length: 591 Molecular Weight: 67360.6 Isoelectric Point: 9.266 |
Chromosome | Chromosome/Scaffold: 10 Start: 8545547 End: 8549317 |
Description | cell wall invertase 4 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 53 | 371 | 0 |
HFQPPKHWINDPNGPLYYKGFYHLFYQYNTKGAVWGNIVWAHSISKDLVNWEALEHALYPSKWFDVKGTWSGSITIVPGKGPIILYTGVNKNETQLQNYA IPEDPSDPYLRKWIKPDDNPIAMPDYTMNGSAFRDPTTAWFSKDGHWRTVVGSRRKHRGIAYIYRSRDFKHWVKAKHPVHSKESTGMWECPDFFPVSTTD FENGLDLDYVGPNTKHVLKVSLDITRFEYYTLGKYDPKKDRYVPDGNSPDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDISKGWAGLQ VIPRTVLLDSNKRHLVFWP |
Full Sequence |
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Protein Sequence Length: 591 Download |
MGVSNIVSVF LLLVLINLSS NNIKGIEAFH KVYEKLQSQS VESVNPLHRS AFHFQPPKHW 60 INDPNGPLYY KGFYHLFYQY NTKGAVWGNI VWAHSISKDL VNWEALEHAL YPSKWFDVKG 120 TWSGSITIVP GKGPIILYTG VNKNETQLQN YAIPEDPSDP YLRKWIKPDD NPIAMPDYTM 180 NGSAFRDPTT AWFSKDGHWR TVVGSRRKHR GIAYIYRSRD FKHWVKAKHP VHSKESTGMW 240 ECPDFFPVST TDFENGLDLD YVGPNTKHVL KVSLDITRFE YYTLGKYDPK KDRYVPDGNS 300 PDGWEGLRFD YGNFYASKTF FDYKKNRRIL WGWANESDTV EDDISKGWAG LQVIPRTVLL 360 DSNKRHLVFW PIEEIESLRG NYVRMNNQNI KMGQRLEVKG ITPAQADVEV TFNIPSLEKA 420 ETFDPSFKLK PLELCKMKGS NVTGGVGPFG LITLATPDLE EYTPVFFRVF KDTSTHKPKV 480 LMCSDARPSS LKQDRGPLKQ DKMYKPSFAG FVDVDLADGR ISLRSLIDHS VIESFGALGK 540 TVITSRVYPV KAVKENAHLY VFNNGTQIVN VESLNAWSMQ RPLQMNNGAQ * 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG1621 | SacC | 2.0e-61 | 30 | 398 | 387 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 7.0e-88 | 59 | 374 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-142 | 53 | 371 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-171 | 53 | 539 | 496 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAM61359.1 | 0 | 21 | 589 | 22 | 590 | putative beta-fructofuranosidase (invertase) [Arabidopsis thaliana] |
GenBank | ABK28599.1 | 0 | 9 | 590 | 9 | 590 | unknown [Arabidopsis thaliana] |
DDBJ | BAC42957.1 | 0 | 9 | 590 | 9 | 590 | putative beta-fructofuranosidase [Arabidopsis thaliana] |
RefSeq | NP_190828.2 | 0 | 9 | 590 | 9 | 590 | AtcwINV2 (Arabidopsis thaliana cell wall invertase 2); hydrolase, hydrolyzing O-glycosyl compounds |
RefSeq | NP_565837.1 | 0 | 21 | 589 | 22 | 590 | AtcwINV4 (Arabidopsis thaliana cell wall invertase 4); hydrolase, hydrolyzing O-glycosyl compounds |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2ac1_A | 0 | 43 | 579 | 3 | 535 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
PDB | 2xqr_K | 0 | 45 | 579 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_I | 0 | 45 | 579 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_G | 0 | 45 | 579 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_E | 0 | 45 | 579 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |