Basic Information | |
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Species | Thellungiella halophila |
Cazyme ID | Thhalv10023331m |
Family | GH32 |
Protein Properties | Length: 656 Molecular Weight: 73537.3 Isoelectric Point: 7.2196 |
Chromosome | Chromosome/Scaffold: 8 Start: 1504321 End: 1508569 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 117 | 437 | 0 |
HFQPEKNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSKDLIHWLYLPFAMVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDKFVQVQNLA YPEDPKDPLLLKWTKFSGNPVLVPPPGIGTKDFRDPTTAWKTSAGKWRITIGSKINRTGISLVYDTTDFKTYEKHDTLLHQVPNTGMWECVDFYPVSKTK ANGLDTSVNGPDVKHIVKASMDDTRIDHYAIGTYYDSNATWVPDNPKIDVGISTGLRYDYGKYYASKTFYDQHKRRRILWGWIGESDSEAADVQKGWSSV QGIPRTVVLDTKTGKNLVQWP |
Full Sequence |
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Protein Sequence Length: 656 Download |
MAITESLLPV TSEQDPLSDS RSDPIPETRR RRPIKIHLAV FSGFLLIALY VALIVTHDGS 60 VSKNGANEDG TVVESRARLA GVSEKRNERI WRFSDDQKTV AFAWNNTMLS WQRTAFHFQP 120 EKNWMNDPNG PLFYKGWYHF FYQYNPNAAV WGDIVWGHAV SKDLIHWLYL PFAMVPDQWY 180 DANGVWTGSA TFLDDGSIVM LYTGSTDKFV QVQNLAYPED PKDPLLLKWT KFSGNPVLVP 240 PPGIGTKDFR DPTTAWKTSA GKWRITIGSK INRTGISLVY DTTDFKTYEK HDTLLHQVPN 300 TGMWECVDFY PVSKTKANGL DTSVNGPDVK HIVKASMDDT RIDHYAIGTY YDSNATWVPD 360 NPKIDVGIST GLRYDYGKYY ASKTFYDQHK RRRILWGWIG ESDSEAADVQ KGWSSVQGIP 420 RTVVLDTKTG KNLVQWPVKE IESLRLSSKK FHMKVEPGTV VPVDVGPAAQ LDIEAEFKIK 480 KDDLEVILGD DSVEDDKEFS CQTSGLSTVR GALGPFGFSV LADQSLSEQT PVYFYVTKGK 540 DSKLRAFLCT DTSRSTKAND VVKPVYGSFV PVLKGEKLTM RILVDHSIVE AFGQGGRTCI 600 TSRVYPTKAI YGATKLFLFN NAIDATVTVS FKVWQMNSAF IHPYSAEDLG VSSRT* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-52 | 113 | 606 | 513 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-71 | 113 | 635 | 535 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 4.0e-94 | 123 | 440 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 9.0e-151 | 117 | 437 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 117 | 596 | 490 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAD43622.1 | 0 | 1 | 655 | 1 | 650 | AC005698_21 T3P18.21 [Arabidopsis thaliana] |
GenBank | AAF19535.1 | 0 | 1 | 650 | 1 | 645 | AC007190_3 F23N19.3 [Arabidopsis thaliana] |
GenBank | AAK62665.1 | 0 | 1 | 655 | 1 | 648 | At1g62660/F23N19_3 [Arabidopsis thaliana] |
EMBL | CAA64781.1 | 0 | 10 | 655 | 1 | 639 | beta-fructosidase [Arabidopsis thaliana] |
RefSeq | NP_564798.1 | 0 | 1 | 655 | 1 | 648 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 100 | 644 | 6 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugh_A | 0 | 100 | 644 | 6 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugg_B | 0 | 100 | 644 | 6 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugg_A | 0 | 100 | 644 | 6 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugf_B | 0 | 100 | 644 | 6 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |