| Basic Information | |
|---|---|
| Species | Volvox carteri |
| Cazyme ID | Vocar20000959m |
| Family | GH32 |
| Protein Properties | Length: 587 Molecular Weight: 64095.5 Isoelectric Point: 5.4885 |
| Chromosome | Chromosome/Scaffold: 16 Start: 1003606 End: 1007660 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 128 | 327 | 1.2e-34 |
| KLTYWTKIEYPWLSLPPPSNGLERLAGWRDPAIISRPGDEGSDCWTMIIGSGVKDSGGSVLVYTSKELLDGWQFHGELCHGRGNGADTGFMWECPILFKL STLPRNASLFGSGGQPDACTNPSYYWLGPYDPVSRRFDLATSQGPYRLDLGNVLYAPNILHDTAKGRTLLWGWGQEQRNKVDVYDYAGCLTLPRVMWLQY | |||
| Full Sequence |
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| Protein Sequence Length: 587 Download |
| MSPTQPTETA ALVRQDRPSF HIAPQQGWMN GDYVQACLSP VTKYSHETVS ADLSKSSLVQ 60 VLPTYSQGLH LAVRPGLGPL CFTRPCQLGA STTRNRMHTW KFRCRWLLQR LRNARRERRS 120 DYPIYDPKLT YWTKIEYPWL SLPPPSNGLE RLAGWRDPAI ISRPGDEGSD CWTMIIGSGV 180 KDSGGSVLVY TSKELLDGWQ FHGELCHGRG NGADTGFMWE CPILFKLSTL PRNASLFGSG 240 GQPDACTNPS YYWLGPYDPV SRRFDLATSQ GPYRLDLGNV LYAPNILHDT AKGRTLLWGW 300 GQEQRNKVDV YDYAGCLTLP RVMWLQYCAD PSTDSSTDSA VCSLALHQQP LPELAELRHP 360 LRSWRLQDAL PLGVMDLIVD GGAKMPVPTV SGPFLDLELV FQPAADCNAD EPRCSASGLL 420 LQSWSSGPEG SAALIYNWDT GILEVVYEAI DPATLTFSLA APGVRRVGGR LQRPPAPGSP 480 LSLRVFLDYS CLEVFTGEGE VLTARVYRGS APTNPGYPAG SSSGIDFISA DGPTRLIHFA 540 AYEMQPAFQS GISSEDEATA SVPLPYESLL EGVERLEMAG AAGGAA* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 2.0e-25 | 156 | 532 | 389 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-31 | 126 | 326 | 204 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-33 | 132 | 326 | 204 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-34 | 126 | 499 | 386 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_001690911.1 | 1e-26 | 126 | 239 | 124 | 232 | glycoside-hydrolase-like protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001690911.1 | 2e-22 | 243 | 401 | 625 | 805 | glycoside-hydrolase-like protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001690911.1 | 1e-35 | 419 | 509 | 873 | 963 | glycoside-hydrolase-like protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001691098.1 | 0 | 13 | 238 | 15 | 258 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | ZP_05902770.1 | 1e-18 | 133 | 526 | 148 | 483 | sucrose-6-phosphate hydrolase [Leptotrichia hofstadii F0254] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qqu_A | 3e-19 | 126 | 507 | 115 | 500 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D239a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2qqw_A | 5e-19 | 126 | 507 | 115 | 500 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2ac1_A | 5e-19 | 126 | 507 | 119 | 504 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 5e-19 | 126 | 507 | 115 | 500 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 5e-19 | 126 | 507 | 115 | 500 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FD803815 | 238 | 287 | 471 | 0 |
| BF864017 | 196 | 345 | 532 | 0 |
| BU649663 | 143 | 243 | 378 | 0 |
| BU649663 | 78 | 385 | 462 | 0 |
| BI729430 | 196 | 386 | 564 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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