| Basic Information | |
|---|---|
| Species | Volvox carteri |
| Cazyme ID | Vocar20004703m |
| Family | CBM20 |
| Protein Properties | Length: 756 Molecular Weight: 78789 Isoelectric Point: 4.6897 |
| Chromosome | Chromosome/Scaffold: 8 Start: 3584964 End: 3591548 |
| Description | catalytics;carbohydrate kinases;phosphoglucan, water dikinases |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 51 | 133 | 3.6e-21 |
| TVTIKVPYRVNYGEVLRVVGSGKVLGDWSADRGLQLIWTEGDVWTVQVPISAGHYEFKCVVYNQATKSVARWEDGGNRILDIT | |||
| Full Sequence |
|---|
| Protein Sequence Length: 756 Download |
| MKALTGVTGR AGEGVTSLSR GRSHREFFMI YRRGGQGAAR SGAPQELTAS TVTIKVPYRV 60 NYGEVLRVVG SGKVLGDWSA DRGLQLIWTE GDVWTVQVPI SAGHYEFKCV VYNQATKSVA 120 RWEDGGNRIL DITRELGTWD VSCQWGATAG MTSTFIPKPQ PPLPAASELE PTATEATTSG 180 DGVAAAEEEL STLMALSETG IDANKVDDVA LQVEAVPQVA SVDAPARSSG DAADTAVAGT 240 SSSSSTGGGG SAALLLFATV AAAGAVAAVA FLGPSGLDKS ILGARAGVAV STTLQQSSLA 300 GEQVRAQLQS QLATLAGHVS AVQPTVQAQM DNGDAEEAGD VIRPVTAGTD VLEDGGVGDE 360 YGDASRPVTA GESNFAEGDE GAAGGDAEPD AEDRPPSARP GSARPQSARP DSAKPEAARP 420 PSARPPSASP DAARPPSARP SSARPFSARP ASASATPPAD GQYAAQAEGA EVEAEGGDGP 480 KPTDDTGADA TTPEAEEDKG AGAGEGSGEE PQGSNHSHHP DVITDINQSP EARAEVDEIA 540 QQQAKEVIRS RQSSACSVRK AGEEEKRRSS ASNTSVRSGP PSRQGPAESL ANMAMQPPSP 600 SKIKTGPWLT GTYRLPKSGK IITGEELCDA LERMTNAYID KYTREKGPSL TKEIAPGAAT 660 VFNTKRLSQD EYDTMIARLY KPKDRNVADG DRIQLITLRF GDDGQEQWVP VKTVSAEEQQ 720 AYMCELYQRC LDNRKKTKED LAAKYLKPLG PPRKK* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02860 | E_set_Pullulanase | 2.0e-10 | 54 | 131 | 80 | + Early set domain associated with the catalytic domain of pullulanase (also called dextrinase and alpha-dextrin endo-1,6-alpha glucosidase). E or "early" set domains are associated with the catalytic domain of pullulanase at either the N-terminal or C-terminal end, and in a few instances at both ends. Pullulanase is an enzyme with activity similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. The E set domain of pullulanase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. | ||
| smart01065 | CBM_2 | 2.0e-13 | 50 | 133 | 89 | + Starch binding domain. | ||
| pfam00686 | CBM_20 | 1.0e-13 | 51 | 130 | 84 | + Starch binding domain. | ||
| cd02861 | E_set_pullulanase_like | 2.0e-14 | 52 | 130 | 80 | + Early set domain associated with the catalytic domain of pullulanase-like proteins. E or "early" set domains are associated with the catalytic domain of pullulanase at either the N-terminal or C-terminal end, and in a few instances at both ends. Pullulanase (also called dextrinase or alpha-dextrin endo-1,6-alpha glucosidase) is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. The E set domain of pullulanase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. | ||
| cd05467 | CBM20 | 1.0e-16 | 52 | 130 | 83 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:2001070 | starch binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAS00635.1 | 0.000000004 | 394 | 474 | 811 | 894 | adhesin [Mycoplasma hyopneumoniae] |
| RefSeq | XP_001459042.1 | 0.0000000006 | 45 | 170 | 10 | 130 | hypothetical protein [Paramecium tetraurelia strain d4-2] |
| RefSeq | XP_001697518.1 | 0.00000000002 | 65 | 167 | 4 | 100 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001698509.1 | 0 | 518 | 754 | 271 | 514 | flagellar associated protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001701978.1 | 0.000000002 | 52 | 127 | 4 | 82 | predicted protein [Chlamydomonas reinhardtii] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1kum_A | 0.01 | 48 | 138 | 5 | 98 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 1kul_A | 0.01 | 48 | 138 | 5 | 98 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 1acz_A | 0.01 | 48 | 138 | 5 | 98 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.01 | 48 | 138 | 5 | 98 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FD860130 | 203 | 554 | 756 | 0 |
| FD826329 | 187 | 514 | 700 | 0 |
| FD816939 | 162 | 514 | 675 | 0 |
| FD824597 | 205 | 124 | 328 | 0 |
| FD931973 | 141 | 514 | 654 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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