| Basic Information | |
|---|---|
| Species | Volvox carteri |
| Cazyme ID | Vocar20005574m |
| Family | CBM20 |
| Protein Properties | Length: 387 Molecular Weight: 41487.9 Isoelectric Point: 4.8896 |
| Chromosome | Chromosome/Scaffold: 11 Start: 2709186 End: 2714309 |
| Description | Carbohydrate-binding-like fold |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 28 | 113 | 4.5e-26 |
| VQVKFCLKYRTSYGQSVKIIGSHPKLGSWDINKALNLDWTDGDRWIAAVELPAGAVYEYKYVLVDHDSRATLAWQGGGNSVLALGD | |||
| Full Sequence |
|---|
| Protein Sequence Length: 387 Download |
| MTAIIGADGK ASSLRKLVVD GVDDSAMVQV KFCLKYRTSY GQSVKIIGSH PKLGSWDINK 60 ALNLDWTDGD RWIAAVELPA GAVYEYKYVL VDHDSRATLA WQGGGNSVLA LGDQDGQGVE 120 VQDNWVGGGG QQHLGCFCIT WSVASGCRGN NPGQAAVVVN GVSTTRESKL QAWANEMRQY 180 RAMARNAQYE LSRKAEELQA SKAQVARLKM ELSVSLKARE DLEARLAEVE SENLALRAQV 240 AQSQIAMKST LEEAIKLLQQ EIEEGEEELY NEEEEEVYVP AAGGNGKTAT SNGASPWSWG 300 RGKEAETVYA GSNGNGSTFE RFGFRLSGNP FDMQGEQAAT SRPSTERASD LGAARVATTS 360 SGSGSSYTGS GHVLADWRTR LSNGGQ* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02950 | PLN02950 | 3.0e-5 | 23 | 111 | 90 | + 4-alpha-glucanotransferase | ||
| cd02859 | E_set_AMPKbeta_like_N | 6.0e-17 | 30 | 125 | 101 | + N-terminal Early set domain, a glycogen binding domain, associated with the catalytic domain of AMP-activated protein kinase beta subunit. E or "early" set domains are associated with the catalytic domain of AMP-activated protein kinase beta subunit glycogen binding domain at the N-terminal end. AMPK is a metabolic stress sensing protein that senses AMP/ATP and has recently been found to act as a glycogen sensor as well. The protein functions as an alpha-beta-gamma heterotrimer. This N-terminal domain is the glycogen binding domain of the beta subunit. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include pullulanase, maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, and isoamylase. | ||
| PLN02950 | PLN02950 | 2.0e-18 | 26 | 131 | 111 | + 4-alpha-glucanotransferase | ||
| pfam00686 | CBM_20 | 6.0e-20 | 27 | 121 | 98 | + Starch binding domain. | ||
| cd05467 | CBM20 | 8.0e-27 | 29 | 125 | 100 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:2001070 | starch binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAL53465.1 | 2e-17 | 22 | 127 | 74 | 181 | glucan 1,4-alpha-glucosidase (ISS) [Ostreococcus tauri] |
| EMBL | CAL56006.1 | 2e-17 | 30 | 127 | 47 | 147 | unnamed protein product [Ostreococcus tauri] |
| GenBank | EEH54486.1 | 5e-16 | 13 | 114 | 103 | 198 | carbohydrate-binding module family 20 protein [Micromonas pusilla CCMP1545] |
| RefSeq | XP_001696825.1 | 0 | 5 | 381 | 1 | 320 | hypothetical protein CHLREDRAFT_150073 [Chlamydomonas reinhardtii] |
| RefSeq | XP_002508103.1 | 2e-18 | 27 | 228 | 290 | 483 | carbohydrate-binding module family 20 protein [Micromonas sp. RCC299] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1kum_A | 0.000002 | 28 | 125 | 7 | 107 | A Chain A, Crystal Structure Of Human Rab43 In Complex With Gdp |
| PDB | 1kul_A | 0.000002 | 28 | 125 | 7 | 107 | A Chain A, Crystal Structure Of Human Rab43 In Complex With Gdp |
| PDB | 1acz_A | 0.000002 | 28 | 125 | 7 | 107 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.000002 | 28 | 125 | 7 | 107 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| PDB | 2vn7_A | 0.00003 | 28 | 106 | 497 | 577 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FD831307 | 167 | 221 | 387 | 0 |
| FD837834 | 107 | 281 | 387 | 0 |
| FD844544 | 67 | 1 | 67 | 1e-36 |
| FD844543 | 53 | 15 | 67 | 1e-26 |
| DC994677 | 95 | 30 | 124 | 8e-22 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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