| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_001215m |
| Family | GT20 |
| Protein Properties | Length: 929 Molecular Weight: 104707 Isoelectric Point: 7.1853 |
| Chromosome | Chromosome/Scaffold: 03542 Start: 27717 End: 38984 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 92 | 559 | 0 |
| RQRLLVVANRLPVSAVRRGEDSWSLEISAGGLVSALLGVKEFEARWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEEIVHQYYNGYCNNMLWPLFHY LGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNKHYEEGDVVWCHDYHLMFLPKCLKEYNSNMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVG FHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFIRALEAPQVQEHIKDLKERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN SHWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQDAKKGVLIL SEFAGAAQSLGAGAILVNPWNITEVADSIRQALTMSCEEREKRHHHNFAHVTTHTAQEWAETFVSELN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 929 Download |
| MPGNQYNGNS TPYPSRTQRL LRERELKKSS RASHSNEVTD NHGGTEPCEH DLRLRERDNS 60 NNSYIDQYVE GAIAATRQLA EGCEKQDGRP LRQRLLVVAN RLPVSAVRRG EDSWSLEISA 120 GGLVSALLGV KEFEARWIGW AGVNVPDEIG QKALTKALAE KRCIPVFLDE EIVHQYYNGY 180 CNNMLWPLFH YLGLPQEDRL ATTRSFQSQF AAYKKANQMF ADVVNKHYEE GDVVWCHDYH 240 LMFLPKCLKE YNSNMKVGWF LHTPFPSSEI HRTLPSRSEL LRSVLAADLV GFHTYDYARH 300 FVSACTRILG LEGTPEGVED QGRLTRVAAF PIGIDSDRFI RALEAPQVQE HIKDLKERFA 360 GRKVMLGVDR LDMIKGIPQK ILAFEKFLEE NSHWRDKVVL LQIAVPTRTD VPEYQKLTSQ 420 VHEIVGRING RFGTLTAVPI HHLDRSLDFH ALCALYAVTD VALVTSLRDG MNLVSYEFVA 480 CQDAKKGVLI LSEFAGAAQS LGAGAILVNP WNITEVADSI RQALTMSCEE REKRHHHNFA 540 HVTTHTAQEW AETFVSELND TVIEAQLRTR QVPPILPEED AIKRYLQSNN RLLILGFNAT 600 LTEPVDTPGR RVDQIKEMEL KLHPELKETL VALCSDPKTT IVVLSGSDSN VLDKNFGEFD 660 MWLAAEHGMF LRFTKGEWMT TMPEHLNMEW VDSVKHVFEY FTERTPRSHF ELRETSLVWN 720 YKYADVEFGR LQARDMLQHL WTGPISNASV DVVQGGRSVE VRAVGVTKGA AIDRILGEIV 780 HSKSMTTPID YVLCIGHFLG KDEDVYTFFE PVLPSDGIGI ARAKQTDGLK SPGERRPSLK 840 LPASKSGSKS SQGKTQRPSP NPDKKLNNHS CGSGRRQSPE KISWNVLDLK GDNYFSCSVG 900 RTRTNARYLL HSSDDVVSFL KKLARASS* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 928 | 932 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 87 | 928 | 842 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 94 | 560 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 0 | 94 | 796 | 710 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| pfam00982 | Glyco_transf_20 | 0 | 93 | 560 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 1 | 926 | 1 | 919 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| EMBL | CAN79485.1 | 0 | 1 | 928 | 1 | 911 | hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002285632.1 | 0 | 1 | 928 | 1 | 925 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 928 | 1 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 928 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 94 | 562 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 94 | 562 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 94 | 562 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 94 | 562 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 94 | 570 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 361 | 455 | 815 | 0 |
| CX546763 | 304 | 81 | 383 | 0 |
| CV711307 | 293 | 92 | 384 | 0 |
| DW069910 | 288 | 180 | 467 | 0 |
| CX545499 | 305 | 397 | 701 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
