| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_001521m |
| Family | GH18 |
| Protein Properties | Length: 865 Molecular Weight: 94829.5 Isoelectric Point: 7.8034 |
| Chromosome | Chromosome/Scaffold: 03581 Start: 766588 End: 773816 |
| Description | Glycosyl hydrolase superfamily protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH18 | 108 | 424 | 4e-33 |
| VLAYITPWNSKGYEMAKRFTNKFTHLSPVWYDLRSQGTELILEGRHNADRGWISELRMKGGALVLPRVVLEAFPSEMLRKKKLRDKAIELVVSECKEMEY DGIVLESWSRWAAYGILSDPDMRNKALKFIKQLGHAIHSVSSSRSSKQPLQLVYVIGPPHSEKLQVHDFGPEDLQSLNDAVDGFSLMTYDFSSPHNPGPN APLKWIHFTLQLLLGTTGNARTLAHKVFLGINFYGNDFLLSEGSGGGAIIGRDYLSLLEKHKPFLRWEENSAEHFFLYFDDENKKHAVFYPSLMSISLRL EEARSWGTGISIWEIGQ | |||
| Full Sequence |
|---|
| Protein Sequence Length: 865 Download |
| MAKRRDRRLV QSSDRREPRG DPSTQVDQFS ESAASDRKLI AIFILFFIVI PAVSVLVYRI 60 KYAPNTYGTE SHARQDGRVK TDLKYEEILS ENSKVSKNAS HRQYTYPVLA YITPWNSKGY 120 EMAKRFTNKF THLSPVWYDL RSQGTELILE GRHNADRGWI SELRMKGGAL VLPRVVLEAF 180 PSEMLRKKKL RDKAIELVVS ECKEMEYDGI VLESWSRWAA YGILSDPDMR NKALKFIKQL 240 GHAIHSVSSS RSSKQPLQLV YVIGPPHSEK LQVHDFGPED LQSLNDAVDG FSLMTYDFSS 300 PHNPGPNAPL KWIHFTLQLL LGTTGNARTL AHKVFLGINF YGNDFLLSEG SGGGAIIGRD 360 YLSLLEKHKP FLRWEENSAE HFFLYFDDEN KKHAVFYPSL MSISLRLEEA RSWGTGISIW 420 EIGQGLDYFF DLLMEGLNAA GLTPISVLSI KRTKPRKLLC HLADSSSVKF SNSASLSTTP 480 HTIQDCLSST FHGSLLLLSS VLSTDLVKAL TYEEALQQST STASSDFDAS GVLDNVISFA 540 TENPAAIAGG AAFLAVPLVL SRILKKPKPW GVVSAAKAYA ALGDDANAQL IDIRAPVELR 600 TVGTPDIRGF KKRPVSVFYK GEDKPGFLKK LSLKFKEPEN TTLFILDKFD GNSELVAELV 660 TFNGFKAAYA IKDGAEGPQG WLNNSLPWKV PSKGWSFDLS SLIDILSGAL GDASGALPLI 720 IGIAAAVGLG VLVFSQVDKI LQLLGLAALV QLAAKLLFAE EREKTLQQVE ELLNTKVAPG 780 ELVDDVKLIG KAFLPPVGSS KALPPPVEPS SEAAADSTVL KAEPASEPAS QKDSVSVSGF 840 QRPLSPYASY PDLKPPTSPT PSQP* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00704 | Glyco_hydro_18 | 2.0e-14 | 182 | 424 | 258 | + Glycosyl hydrolases family 18. | ||
| COG3858 | COG3858 | 7.0e-17 | 185 | 438 | 260 | + Predicted glycosyl hydrolase [General function prediction only] | ||
| cd02874 | GH18_CFLE_spore_hydrolase | 8.0e-25 | 108 | 433 | 348 | + Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis. | ||
| smart00636 | Glyco_18 | 1.0e-27 | 108 | 424 | 354 | + Glyco_18 domain. | ||
| cd02876 | GH18_SI-CLP | 5.0e-148 | 104 | 433 | 335 | + Stabilin-1 interacting chitinase-like protein (SI-CLP) is a eukaryotic chitinase-like protein of unknown function that interacts with the endocytic/sorting transmembrane receptor stabilin-1 and is secreted from the lysosome. SI-CLP has a glycosyl hydrolase family 18 (GH18) domain but lacks a chitin-binding domain. The catalytic amino acids of the GH18 domain are not conserved in SI-CLP, similar to the chitolectins YKL-39, YKL-40, and YM1/2. Human SI-CLP is sorted to late endosomes and secretory lysosomes in alternatively activated macrophages. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI40499.1 | 0 | 1 | 433 | 1 | 435 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_192013.1 | 0 | 1 | 433 | 1 | 430 | glycosyl hydrolase family 18 protein [Arabidopsis thaliana] |
| RefSeq | XP_002320856.1 | 0 | 61 | 433 | 27 | 399 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002523373.1 | 0 | 1 | 433 | 1 | 432 | chitinase, putative [Ricinus communis] |
| RefSeq | XP_002523373.1 | 0 | 434 | 854 | 507 | 928 | chitinase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3bxw_A | 0 | 77 | 433 | 49 | 393 | B Chain B, Crystal Structure Of Stabilin-1 Interacting Chitinase-Like Protein, Si-Clp |
| PDB | 3bxw_B | 0 | 77 | 433 | 49 | 393 | B Chain B, Crystal Structure Of Stabilin-1 Interacting Chitinase-Like Protein, Si-Clp |
| PDB | 2dcq_A | 0 | 575 | 693 | 9 | 127 | B Chain B, Crystal Structure Of Stabilin-1 Interacting Chitinase-Like Protein, Si-Clp |
| PDB | 1vee_A | 0 | 575 | 693 | 9 | 127 | A Chain A, Nmr Structure Of The Hypothetical Rhodanese Domain At4g01050 From Arabidopsis Thaliana |
| PDB | 2fsx_A | 0.000006 | 571 | 689 | 5 | 138 | A Chain A, Crystal Structure Of Rv0390 From M. Tuberculosis |
