| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_001558m |
| Family | GT20 |
| Protein Properties | Length: 861 Molecular Weight: 96778.5 Isoelectric Point: 5.3941 |
| Chromosome | Chromosome/Scaffold: 04437 Start: 69003 End: 74635 |
| Description | trehalose phosphatase/synthase 5 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 57 | 547 | 0 |
| SQERMIIVGNQLPLRVHQSPDGNGEWCFSWDEDSLLLQLRDGLGEDVEVIYVGCLKEEVDPSEQDDVAQTLLESFKCVPAFIPPELFSKYYHGFCKQHLW PLFHYMLPLSPDLGGRFDRSLWQAYVSVNKIFADKVMEVISPDDDYVWVHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSSEIYRTLPVRDELLRALLNA DLIGFHTFDYARHFLSCCSRMLGLSYQSKRGYIGLEYYGRTVSIKILPVGIHIGQLQSVLNLPETESKVAELHDQFRGQTVILGVDDMDIFKGISLKLLA MEQLLLQHPDKRGEVVLVQIANPARGRGRDVQEVQSETKATVRRINEIFGRPGYAPVVLIDTPLQFYERIAYYVIAECCLVTAVRDGMNLIPYEYVICRQ GNDKLDETLGLNTFDPKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVAEAMDSALVIPEPEKQMRHEKHYRYVSTHDVAYWARSFLQDLER | |||
| Full Sequence |
|---|
| Protein Sequence Length: 861 Download |
| MVSRSYSNLL DLASGESPTF GRERKRLPRV ATVAGVLSEL EDENSNSVGS DAPSSVSQER 60 MIIVGNQLPL RVHQSPDGNG EWCFSWDEDS LLLQLRDGLG EDVEVIYVGC LKEEVDPSEQ 120 DDVAQTLLES FKCVPAFIPP ELFSKYYHGF CKQHLWPLFH YMLPLSPDLG GRFDRSLWQA 180 YVSVNKIFAD KVMEVISPDD DYVWVHDYHL MVLPTFLRKR FNRVKLGFFL HSPFPSSEIY 240 RTLPVRDELL RALLNADLIG FHTFDYARHF LSCCSRMLGL SYQSKRGYIG LEYYGRTVSI 300 KILPVGIHIG QLQSVLNLPE TESKVAELHD QFRGQTVILG VDDMDIFKGI SLKLLAMEQL 360 LLQHPDKRGE VVLVQIANPA RGRGRDVQEV QSETKATVRR INEIFGRPGY APVVLIDTPL 420 QFYERIAYYV IAECCLVTAV RDGMNLIPYE YVICRQGNDK LDETLGLNTF DPKKSMLVVS 480 EFIGCSPSLS GAIRVNPWNI DAVAEAMDSA LVIPEPEKQM RHEKHYRYVS THDVAYWARS 540 FLQDLERACR DHVRRRCWGI GFGLGFRVIA LDPNFRKLSV EHIVSAYKRT KHRAILLDYD 600 GTMILSGSIS TAPSTEAVGI LNNLCRDPKN VVFVVSGKDK ETLAEWFSSS EKLGIAAEHG 660 YFVRPNHDVD WETCVSVPDF DWKQIAEPVM KLYTETTDGS AIETKESALV WNYQYADPDF 720 GSCQAKELLD HLESVLTNEP VSVKSGQHIV EVKPQGVNKG LVAQCLLETM QKKGMLPDFV 780 LCIGDDRSDE DMFEVIMSAR AGPSLSPVAE VFACTVGQKP SKAKYYLEDT SEILRMLEGL 840 ANASELAARS SPPIGAKTDH * 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 4.0e-167 | 58 | 852 | 838 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 845 | 849 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 59 | 547 | 493 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| cd03788 | GT1_TPS | 0 | 60 | 546 | 487 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| PRK14501 | PRK14501 | 0 | 60 | 843 | 794 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN61971.1 | 0 | 1 | 839 | 1 | 839 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_567538.1 | 0 | 1 | 841 | 1 | 840 | ATTPS5; protein binding / transferase, transferring glycosyl groups / trehalose-phosphatase [Arabidopsis thaliana] |
| RefSeq | XP_002277503.1 | 0 | 1 | 856 | 1 | 832 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002304347.1 | 0 | 1 | 852 | 1 | 850 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527658.1 | 0 | 1 | 849 | 1 | 794 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 143 | 547 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 143 | 547 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 143 | 547 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 143 | 547 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 143 | 547 | 72 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 768 | 82 | 845 | 0 |
| HO779327 | 513 | 102 | 614 | 0 |
| HO779327 | 238 | 612 | 849 | 0 |
| HO797286 | 485 | 377 | 861 | 0 |
| HO777775 | 83 | 1 | 82 | 0.000000009 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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