| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_001828m |
| Family | GT47 |
| Protein Properties | Length: 814 Molecular Weight: 91053.1 Isoelectric Point: 6.3491 |
| Chromosome | Chromosome/Scaffold: 06908 Start: 231779 End: 243330 |
| Description | DHFS-FPGS homolog B |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GT47 | 49 | 283 | 2.69999e-40 |
| AFKIYMYTPPKPFSFTSPVESLFFSSLSNSPFVTQDPEEAHLFFVPLSANLSTRTIARVIRELRMEFPYWNRTLGADHFYVSCQGLGYESDRNLVELKKN SVQISCFPAPEGKFVPHKDITLPPLAFAHSTQVPANRTVKYRGFVKHNGVKESALVNELRSASDFFMENQPSDEKTFVDRLANSEFCLFEYGADVSGIAD ALRFGCVPVVISGRPIQDLPLMDVMRWQEIAVFVG | |||
| Full Sequence |
|---|
| Protein Sequence Length: 814 Download |
| MDINYFSFFL CFTFFTSSFH VHSKTITGSS SLYLSPTILF PNYQKMLEAF KIYMYTPPKP 60 FSFTSPVESL FFSSLSNSPF VTQDPEEAHL FFVPLSANLS TRTIARVIRE LRMEFPYWNR 120 TLGADHFYVS CQGLGYESDR NLVELKKNSV QISCFPAPEG KFVPHKDITL PPLAFAHSTQ 180 VPANRTVKYR GFVKHNGVKE SALVNELRSA SDFFMENQPS DEKTFVDRLA NSEFCLFEYG 240 ADVSGIADAL RFGCVPVVIS GRPIQDLPLM DVMRWQEIAV FVGYKSNGGL DLEELKGVLD 300 RTCKGNTGSP KRVVTPYDEA MDALSSLITK RSRADKSNKG DRFDLLFDYL KMLELDQAIS 360 EMKIIHVAGT KGKGSTCTFT ESILRHCGFH TGLFTSPHLI DVRERFRLDG EDICEEKFLA 420 YFWWCYDRLK VDVAILEVGL GGTFDATNVV QTHVVCGISS LGYDHMEILG NTLGEIAGEK 480 AGIFKNGVPA FTVPQPDEAM RVLEEKASKR DVPLQVAPPL DSNLLNGLKL GLEGEHQYIN 540 AGLAVALSSK WLQRTGHLEI SYLEQTSSLP EQFIKGLTTA CLQGRAQIVP DRCINNESDG 600 DLVFYLDGAH SPESMEVCAR WFSLAIKDDN PQNTLNCPSQ NNFQSMLECV DKYHDGKSGK 660 NLMQILLFNC MSVRDPQLLL PRLMKTCATH GVYFKKALFV PNISVYYKVG SHALPPTEPQ 720 VDLSWQFTLQ RLWENLVQGD KGGEANNTDA VCEEVKDDTG SSVRTCKNSA VFPSLPLAIK 780 WLRDSVYQNR SVRIQVLVTG SLHLVGDVLR LVK* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK10846 | PRK10846 | 3.0e-29 | 363 | 612 | 287 | + bifunctional folylpolyglutamate synthase/ dihydrofolate synthase; Provisional | ||
| COG0285 | FolC | 8.0e-76 | 317 | 622 | 346 | + Folylpolyglutamate synthase [Coenzyme metabolism] | ||
| TIGR01499 | folC | 8.0e-86 | 352 | 811 | 504 | + folylpolyglutamate synthase/dihydrofolate synthase. This model represents the FolC family of folate pathway proteins. Most examples are bifunctional, active as both folylpolyglutamate synthetase (EC 6.3.2.17) and dihydrofolate synthetase (EC 6.3.2.12). The two activities are similar - ATP + glutamate + dihydropteroate or tetrahydrofolyl-[Glu](n) = ADP + orthophosphate + dihydrofolate or tetrahydrofolyl-[Glu](n+1). A mutation study of the FolC gene of E. coli suggests that both activities belong to the same active site. Because some examples are monofunctional (and these cannot be separated phylogenetically), the model is treated as subfamily, not equivalog [Biosynthesis of cofactors, prosthetic groups, and carriers, Folic acid]. | ||
| PLN02881 | PLN02881 | 0 | 306 | 813 | 536 | + tetrahydrofolylpolyglutamate synthase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004326 | tetrahydrofolylpolyglutamate synthase activity |
| GO:0005524 | ATP binding |
| GO:0009058 | biosynthetic process |
| GO:0009396 | folic acid-containing compound biosynthetic process |
| GO:0016020 | membrane |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI31610.1 | 0 | 297 | 813 | 78 | 617 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002277686.1 | 0 | 305 | 813 | 4 | 538 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002311192.1 | 0 | 306 | 813 | 6 | 538 | folylpolyglutamate synthase [Populus trichocarpa] |
| RefSeq | XP_002316257.1 | 0 | 306 | 813 | 6 | 580 | folylpolyglutamate synthase [Populus trichocarpa] |
| RefSeq | XP_002524236.1 | 0 | 304 | 813 | 3 | 530 | folylpolyglutamate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1o5z_A | 4e-29 | 361 | 624 | 51 | 338 | A Chain A, Crystal Structure Of Folylpolyglutamate Synthase (Tm0166) From Thermotoga Maritima At 2.10 A Resolution |
| PDB | 3qcz_A | 4e-26 | 363 | 647 | 53 | 351 | A Chain A, Crystal Structure Of Folylpolyglutamate Synthase (Tm0166) From Thermotoga Maritima At 2.10 A Resolution |
| PDB | 3pyz_A | 4e-26 | 363 | 647 | 53 | 351 | A Chain A, Crystal Structure Of Folylpolyglutamate Synthase (Tm0166) From Thermotoga Maritima At 2.10 A Resolution |
| PDB | 3nrs_A | 4e-26 | 363 | 647 | 53 | 351 | A Chain A, Crystal Structure Of Folylpolyglutamate Synthase (Tm0166) From Thermotoga Maritima At 2.10 A Resolution |
| PDB | 3n2a_A | 4e-26 | 363 | 647 | 53 | 351 | A Chain A, Crystal Structure Of Bifunctional Folylpolyglutamate SynthaseDIHYDROFOLATE SYNTHASE FROM YERSINIA PESTIS CO92 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| folate polyglutamylation | FOLYLPOLYGLUTAMATESYNTH-RXN | EC-6.3.2.17 | tetrahydrofolate synthase |
| folate polyglutamylation | FORMYLTHFGLUSYNTH-RXN | EC-6.3.2.17 | tetrahydrofolate synthase |
| tetrahydrofolate biosynthesis II | FOLYLPOLYGLUTAMATESYNTH-RXN | EC-6.3.2.17 | tetrahydrofolate synthase |
