| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_002192m |
| Family | GH31 |
| Protein Properties | Length: 762 Molecular Weight: 85170.4 Isoelectric Point: 5.4892 |
| Chromosome | Chromosome/Scaffold: 11834 Start: 41824 End: 48952 |
| Description | heteroglycan glucosidase 1 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 174 | 584 | 0 |
| SPTAVLQSLSHAIGTVFMPPKWALGYQQCRWSYDSAQKVMEIAKTFREKGIPCDVIWMDIDYMDGYRCFTFHQGRFPNPQSLANDLHNLGFKATWMLDPG IKCDEGYFVYDSGSESDVWVQSADGTPFTGEVWPGLCVFPDFTQSKARSWWAGLVKDFISNGVDGIWNDMNEPAVQNVQSKTMPESNIHRGDDELGGHQE HSHYHNVYGMLMARSTFEGMKLANENKRTFVLTRAGFIGSQRYAATWTGDNVSNWLHLHMSISMVLQLGISGQPFSGPDIGGFAEDATPRLFARWMGIGA MFPFSRGHSSKETKDHEPWSFGKECEEVCRLALKRRYRLIPHLYTLFYTAHTMGTPVATPTFFADTKDTSLRTLENAFLLGPLLVYASTIPDQGTDKLQY HLPKGIWLRFD | |||
| Full Sequence |
|---|
| Protein Sequence Length: 762 Download |
| MSGNEENTVN SDAKSGKMFF EPILEDGVFR FDCSSIDRAA AYPSLSFINT KLRDTLFNVP 60 SYIPTFECNS GNQIVKLELP KGTSFYGTGE VSGPLERTGK KIETWNTDSY AYGPETMPLY 120 QSHPWVLAIL PNGEAIGVLA DTTKRCEIDL TTVSIIQFTA SASYPVITFG PFASPTAVLQ 180 SLSHAIGTVF MPPKWALGYQ QCRWSYDSAQ KVMEIAKTFR EKGIPCDVIW MDIDYMDGYR 240 CFTFHQGRFP NPQSLANDLH NLGFKATWML DPGIKCDEGY FVYDSGSESD VWVQSADGTP 300 FTGEVWPGLC VFPDFTQSKA RSWWAGLVKD FISNGVDGIW NDMNEPAVQN VQSKTMPESN 360 IHRGDDELGG HQEHSHYHNV YGMLMARSTF EGMKLANENK RTFVLTRAGF IGSQRYAATW 420 TGDNVSNWLH LHMSISMVLQ LGISGQPFSG PDIGGFAEDA TPRLFARWMG IGAMFPFSRG 480 HSSKETKDHE PWSFGKECEE VCRLALKRRY RLIPHLYTLF YTAHTMGTPV ATPTFFADTK 540 DTSLRTLENA FLLGPLLVYA STIPDQGTDK LQYHLPKGIW LRFDFDDSHP DLPTLYLQGG 600 FIIPLGPPHQ HVDEAKFSDD LTLIVALDEN GKAKGTLFED AGDGYEFTKG GYLLTHYVAE 660 RHSIVIKVCE RELQFSIVTV RVSETKGYWK RPKRRLHVQL LLGGGAKIDS WGIDGEDVII 720 AVPSDFEISE LVSVSEKLYK TRLESYKQIP DVKEVQGPDG A* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06603 | GH31_GANC_GANAB_alpha | 5.0e-133 | 187 | 527 | 345 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
| COG1501 | COG1501 | 2.0e-139 | 71 | 690 | 643 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
| pfam01055 | Glyco_hydro_31 | 3.0e-168 | 167 | 603 | 455 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| PLN02763 | PLN02763 | 0 | 15 | 761 | 750 | + hydrolase, hydrolyzing O-glycosyl compounds | ||
| cd06604 | GH31_glucosidase_II_MalA | 0 | 187 | 527 | 342 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI37476.1 | 0 | 1 | 760 | 67 | 817 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_566736.1 | 0 | 1 | 760 | 3 | 751 | HGL1 (heteroglycan glucosidase 1); hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| RefSeq | XP_002263148.1 | 0 | 18 | 760 | 1 | 733 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002326592.1 | 0 | 4 | 761 | 5 | 761 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002519886.1 | 0 | 1 | 761 | 1 | 752 | neutral alpha-glucosidase ab precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2g3n_F | 0 | 68 | 605 | 46 | 610 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 2g3n_E | 0 | 68 | 605 | 46 | 610 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 2g3n_D | 0 | 68 | 605 | 46 | 610 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 2g3n_C | 0 | 68 | 605 | 46 | 610 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 2g3n_B | 0 | 68 | 605 | 46 | 610 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO779383 | 422 | 342 | 760 | 0 |
| HO783906 | 317 | 224 | 540 | 0 |
| EL478108 | 288 | 107 | 394 | 0 |
| EG431953 | 280 | 321 | 600 | 0 |
| HO783906 | 77 | 470 | 546 | 1.6 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
