| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_002466m |
| Family | GT35 |
| Protein Properties | Length: 728 Molecular Weight: 81398.9 Isoelectric Point: 8.4724 |
| Chromosome | Chromosome/Scaffold: 11341 Start: 923485 End: 930356 |
| Description | alpha-glucan phosphorylase 2 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 323 | 722 | 0 |
| PPKLVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNAFYEVWPNKFQNKTNGVTPRRWIRFCNPDLSKIITEWTGSEEWVLNTEKLAELRKFADNEDFQTQ WRAAKKSNKMKVASLLKEKTGYSVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAVERKAKYVPRVCIFGGKAFAPYAQAKRIVKFITDVGAT VNHDPEIGDLLKVVFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGEDNFFLFGAKAHEIAGLRKERAMGK FVPDPRFEEVKDFIRTGVFGSCNYDELLGSLEGNEGFGRADYFLVGKDFPSYVECQEKVDEAYQDQRRWTNMSIMNTAGSYKFSSDRTIHEYAKDIWNIK | |||
| Full Sequence |
|---|
| Protein Sequence Length: 728 Download |
| MATTTSHFSA TSHCNSVSKL VDFGSRSGGK WRPNLLLIRT LRSRPHTRSF SVKNASGEQK 60 TKPKNPISDG DASSIASSIK NHAEFTPLFS PEKFEVPKAF VATAQSVRDA LIINWNATYE 120 CYDRLNVKQA YYLSMEFLQG RALLNAIGNL ELTGAYAEAL TKLGQDLESV ARREPDAALG 180 NGGLGRLASC FLDSMATLNY PAWGYGLRYK YGLFKQRITK DGQEEVAEDW LELVKFIVCK 240 YGKADPNILE KKLKEMRILE NVDLPSSFAG LTLKPKESSA AAISEASKLN VKDDVDSGDE 300 PQSKGEPKSK GTQKKEEVMA EPPPKLVRMA NLCVVGGHAV NGVAEIHSEI VKDEVFNAFY 360 EVWPNKFQNK TNGVTPRRWI RFCNPDLSKI ITEWTGSEEW VLNTEKLAEL RKFADNEDFQ 420 TQWRAAKKSN KMKVASLLKE KTGYSVSPDA MFDIQVKRIH EYKRQLLNIL GIVYRYKKMK 480 EMSAVERKAK YVPRVCIFGG KAFAPYAQAK RIVKFITDVG ATVNHDPEIG DLLKVVFVPD 540 YNVSVAELLI PASELSQHIS TAGMEASGTS NMKFAMNGCI LIGTLDGANV EIRQEVGEDN 600 FFLFGAKAHE IAGLRKERAM GKFVPDPRFE EVKDFIRTGV FGSCNYDELL GSLEGNEGFG 660 RADYFLVGKD FPSYVECQEK VDEAYQDQRR WTNMSIMNTA GSYKFSSDRT IHEYAKDIWN 720 IKPILLP* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 1.0e-22 | 159 | 232 | 74 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 3.0e-51 | 80 | 232 | 154 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 318 | 721 | 409 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 318 | 723 | 411 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 307 | 721 | 420 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 232 | 727 | 420 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| RefSeq | XP_002305367.1 | 0 | 39 | 233 | 5 | 210 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002305367.1 | 0 | 232 | 727 | 431 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 4 | 233 | 7 | 247 | glycogen phosphorylase, putative [Ricinus communis] |
| RefSeq | XP_002526085.1 | 0 | 232 | 727 | 468 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1z8d_A | 0 | 314 | 723 | 427 | 830 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 1z8d_A | 1e-24 | 71 | 231 | 24 | 184 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4el5_A | 0 | 314 | 723 | 415 | 818 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4el5_A | 2e-24 | 71 | 231 | 12 | 172 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4el0_A | 0 | 314 | 723 | 415 | 818 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778303 | 509 | 232 | 728 | 0 |
| HO778303 | 135 | 99 | 233 | 0 |
| HO778303 | 22 | 80 | 101 | 0 |
| HO797178 | 401 | 328 | 728 | 0 |
| HO613954 | 403 | 326 | 728 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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