| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_002572m |
| Family | GT20 |
| Protein Properties | Length: 718 Molecular Weight: 82025.6 Isoelectric Point: 7.4808 |
| Chromosome | Chromosome/Scaffold: 10173 Start: 736886 End: 740500 |
| Description | UDP-Glycosyltransferase / trehalose-phosphatase family protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 57 | 551 | 0 |
| IQKDRIIIVANQLPIRAQRKSDGSKSWIFTWDENSLLLQLKDGLGDDDIEVIYVGCLREDIHPSEQDEVSQILLETFKCVPTFLPPDLFSRYYHGFCKQQ LWPLFHYMLPLSPELGGRFNRSLWQAYVSANKIFADRIMEVINPEDDFVWVHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSSEIYKTLPIREELLRALL NSDLIGFHTFDYARHFLSCCSRMLGLTYESRRGYIGLDYCGRTVSIKILPVGIHMGQLQSVLRLPETETKVMKLIKQFGDRGRIMLLGVDDMDIFKGISL KLLAMEQLLVQHPEWRGKVVLVQIANPARGRGKDVKEVQAETYSTVKRINEMFGKPGYDPVVLIDAPLKFYEKVAYYVVAECCLVTAVRDGMNLIPYEYI ISRQGNEQLDKVLRLEACTPKKSMLVISEFIGCSPSLSGAIRVNPWNIDAVADAMDYALEMAEPEKQLRHDKHYKYVSTHDVGYWARSFLQDLER | |||
| Full Sequence |
|---|
| Protein Sequence Length: 718 Download |
| MVSRSYSNLL ELASGESPSF GRMTRRIPRI MTVAGIISDL DDDPSASVCS DPSSSSIQKD 60 RIIIVANQLP IRAQRKSDGS KSWIFTWDEN SLLLQLKDGL GDDDIEVIYV GCLREDIHPS 120 EQDEVSQILL ETFKCVPTFL PPDLFSRYYH GFCKQQLWPL FHYMLPLSPE LGGRFNRSLW 180 QAYVSANKIF ADRIMEVINP EDDFVWVHDY HLMVLPTFLR KRFNRVKLGF FLHSPFPSSE 240 IYKTLPIREE LLRALLNSDL IGFHTFDYAR HFLSCCSRML GLTYESRRGY IGLDYCGRTV 300 SIKILPVGIH MGQLQSVLRL PETETKVMKL IKQFGDRGRI MLLGVDDMDI FKGISLKLLA 360 MEQLLVQHPE WRGKVVLVQI ANPARGRGKD VKEVQAETYS TVKRINEMFG KPGYDPVVLI 420 DAPLKFYEKV AYYVVAECCL VTAVRDGMNL IPYEYIISRQ GNEQLDKVLR LEACTPKKSM 480 LVISEFIGCS PSLSGAIRVN PWNIDAVADA MDYALEMAEP EKQLRHDKHY KYVSTHDVGY 540 WARSFLQDLE RTCRDHARRR CWGIGFGLSF RVVALDPNFR KLSMEHIVSA YKRTTTRAIL 600 LDYDGTLMPQ ASIDKSPSPK SIDILNSLCR DKNNMVFLVS ARSRKTLTEW FSQCEKLGLA 660 AEHGYFRRLT RDAEWETCVP VTDTAWKQIA EPVMHLYTET TDGSTIEDKE TAQRACG* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 1.0e-146 | 59 | 712 | 679 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PRK14501 | PRK14501 | 2.0e-163 | 61 | 712 | 658 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 716 | 716 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 61 | 551 | 493 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| cd03788 | GT1_TPS | 0 | 61 | 550 | 490 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG52003.1 | 0 | 1 | 712 | 1 | 705 | AC012563_13 putative trehalose-6-phosphate synthase; 46897-44149 [Arabidopsis thaliana] |
| RefSeq | NP_974105.1 | 0 | 1 | 712 | 1 | 718 | ATTPS6; alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups / trehalose-phosphatase [Arabidopsis thaliana] |
| RefSeq | XP_002284972.1 | 0 | 1 | 716 | 1 | 716 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002312472.1 | 0 | 1 | 716 | 1 | 716 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002314777.1 | 0 | 1 | 716 | 1 | 702 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 148 | 551 | 75 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 148 | 551 | 75 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 148 | 551 | 75 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 148 | 551 | 75 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 148 | 551 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 642 | 76 | 716 | 0 |
| HO779327 | 515 | 106 | 620 | 0 |
| DY290000 | 355 | 171 | 523 | 0 |
| HO779327 | 99 | 614 | 712 | 6e-27 |
| HO777775 | 78 | 1 | 78 | 2e-16 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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