y
Basic Information | |
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Species | Manihot esculenta |
Cazyme ID | cassava4.1_003183m |
Family | GH32 |
Protein Properties | Length: 661 Molecular Weight: 73473.6 Isoelectric Point: 5.3707 |
Chromosome | Chromosome/Scaffold: 11581 Start: 118337 End: 121973 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 136 | 454 | 0 |
HFQPEKNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSRDLIHWLHLPLAMVADQWYDQNGVWTGSATILPDGKIVMLYTGSTNESVQVQNLA YPADPNDPLLLDWVKYSGNPVLVPPPGIGTKDFRDPTTAWYTSEGKWRISIGSKIGKTGIALIYDTEDFINYKLQPQALHGVPGTGMWECVDFYPVSRTS QNGVDTSATGPEVKHVVKASLDDDRHDYYALGTYNEVTSTWTPDNPEIDVGIGLRYDYGIFYASKTFYDQNKGRRVLWGWIGESDSEVADVKKGWASLQG IPRTVTLDTKTGSNLLQWP |
Full Sequence |
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Protein Sequence Length: 661 Download |
MADPNPFLPV SQPLQPTYTS LPDGTHPSGS PPTHCIPSKK LLLGIFSGLV MVFLFVALIG 60 NRNGSQLNIY PQQDENVVSL ASPTETAKPE TLRPISRGKS AGVSEKANLI SGVSESSTDQ 120 YPWNNSMLSW QRTAFHFQPE KNWMNDPNGP LFYKGWYHFF YQYNPNAAVW GDIVWGHAVS 180 RDLIHWLHLP LAMVADQWYD QNGVWTGSAT ILPDGKIVML YTGSTNESVQ VQNLAYPADP 240 NDPLLLDWVK YSGNPVLVPP PGIGTKDFRD PTTAWYTSEG KWRISIGSKI GKTGIALIYD 300 TEDFINYKLQ PQALHGVPGT GMWECVDFYP VSRTSQNGVD TSATGPEVKH VVKASLDDDR 360 HDYYALGTYN EVTSTWTPDN PEIDVGIGLR YDYGIFYASK TFYDQNKGRR VLWGWIGESD 420 SEVADVKKGW ASLQGIPRTV TLDTKTGSNL LQWPVEEVES LRLRSNEFNK VEVKPGSVVP 480 LDLDAATQLD IVAEFELDKK ALEKTAESNE EFSCTTSHGA RHRNALGPFG LLVLADDSLV 540 EQTPVYFYVQ KSNGTLKTFF CTDQSRSSAA NDVNKQIYGN FVPVLEGEKF TLRVLVDHSI 600 IESFAQGGRT TISSRVYPTR AIYGSAKLFL FNNAIEADVT ASLKIWQMNS AFIRPYPNTR 660 * 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 8.0e-55 | 132 | 618 | 501 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 6.0e-74 | 113 | 620 | 527 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 9.0e-93 | 142 | 457 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 4.0e-149 | 136 | 454 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 136 | 608 | 483 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB82419.1 | 0 | 84 | 658 | 74 | 646 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 84 | 658 | 74 | 646 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35859.1 | 0 | 1 | 656 | 1 | 649 | soluble acid invertase [Pyrus pyrifolia var. culta] |
RefSeq | XP_002303519.1 | 0 | 1 | 660 | 1 | 661 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532576.1 | 0 | 1 | 660 | 1 | 649 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 121 | 656 | 8 | 544 | A Chain A, Solution Structure Of The C-Terminal Gal-Bind Lectin Domain From Human Galectin-4 |
PDB | 3ugh_A | 0 | 121 | 656 | 8 | 544 | A Chain A, Solution Structure Of The C-Terminal Gal-Bind Lectin Domain From Human Galectin-4 |
PDB | 3ugg_B | 0 | 121 | 656 | 8 | 544 | A Chain A, Solution Structure Of The C-Terminal Gal-Bind Lectin Domain From Human Galectin-4 |
PDB | 3ugg_A | 0 | 121 | 656 | 8 | 544 | A Chain A, Solution Structure Of The C-Terminal Gal-Bind Lectin Domain From Human Galectin-4 |
PDB | 3ugf_B | 0 | 121 | 656 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |