| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_003508m |
| Family | PL4 |
| Protein Properties | Length: 635 Molecular Weight: 72446.1 Isoelectric Point: 6.2454 |
| Chromosome | Chromosome/Scaffold: 08994 Start: 173910 End: 183042 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 8 | 616 | 0 |
| LYIQDHHVVMDNGIVQVTLSNPGGIVTGIRYNGIDNLLEVLNRETNRGYWDLVWNPPGGKGIFDVISGTSFKVIVENEEQVELSFTRMWDPSLEGKYIPL NIDKRFILLRGSSGFYSYAIYEHLKEWPGFELGETRITFKLRKDKFQYMAVGDNLQRLMPLPDDRMPGRCQTLAYPEAVLLVNPKHPELKGEVDDKYQYS CNNEDNQVHGWISFNPPVGFWQITPSNEFRTGGPLKQNLTSHVGPTTLAMFHSSHYAGKDLLPIYNPGEYWKKVFGPVFIYLNSASTGNDSLFLWQDAKI KMMAEVQSWPYSFPASADFQKSAERGNVFGRLLVQDRYMSEDYIVASGAYVGMAPPGDVGSWQRECKDYQFWTRADENGCFFIKNIRTGDYNLYAWVPGF IGDYQFEAVVTIISGCNIYMGDLVYNPPRDGPTLWEIGIPDRSAAEFYVPDPDPMHVNKLFINHPDRFRQYGLWSRYVEIYPEADLVYTVGVSDHHKDWF FAQVVRRKDDGTHVGTTWQIKFKLDNVDQRNTYKLRVALASATLAELQVRVNDPKAARPLFTTGLIGRDNSIARHGIHGLYWLYNVNIPGVRLVEGENTV FLTQPRCTS | |||
| Full Sequence |
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| Protein Sequence Length: 635 Download |
| MPPPAVRLYI QDHHVVMDNG IVQVTLSNPG GIVTGIRYNG IDNLLEVLNR ETNRGYWDLV 60 WNPPGGKGIF DVISGTSFKV IVENEEQVEL SFTRMWDPSL EGKYIPLNID KRFILLRGSS 120 GFYSYAIYEH LKEWPGFELG ETRITFKLRK DKFQYMAVGD NLQRLMPLPD DRMPGRCQTL 180 AYPEAVLLVN PKHPELKGEV DDKYQYSCNN EDNQVHGWIS FNPPVGFWQI TPSNEFRTGG 240 PLKQNLTSHV GPTTLAMFHS SHYAGKDLLP IYNPGEYWKK VFGPVFIYLN SASTGNDSLF 300 LWQDAKIKMM AEVQSWPYSF PASADFQKSA ERGNVFGRLL VQDRYMSEDY IVASGAYVGM 360 APPGDVGSWQ RECKDYQFWT RADENGCFFI KNIRTGDYNL YAWVPGFIGD YQFEAVVTII 420 SGCNIYMGDL VYNPPRDGPT LWEIGIPDRS AAEFYVPDPD PMHVNKLFIN HPDRFRQYGL 480 WSRYVEIYPE ADLVYTVGVS DHHKDWFFAQ VVRRKDDGTH VGTTWQIKFK LDNVDQRNTY 540 KLRVALASAT LAELQVRVND PKAARPLFTT GLIGRDNSIA RHGIHGLYWL YNVNIPGVRL 600 VEGENTVFLT QPRCTSPFQG LMYDYIRLEG PPSS* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd10316 | RGL4_M | 2.0e-29 | 331 | 430 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 1.0e-52 | 442 | 629 | 190 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 1.0e-79 | 14 | 294 | 287 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 3.0e-103 | 1 | 200 | 200 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI19298.1 | 0 | 1 | 633 | 1 | 646 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002285626.1 | 0 | 17 | 633 | 1 | 617 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
| RefSeq | XP_002306520.1 | 0 | 17 | 632 | 1 | 616 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527352.1 | 0 | 1 | 631 | 1 | 631 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527353.1 | 0 | 1 | 634 | 1 | 636 | lyase, putative [Ricinus communis] |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GW864372 | 311 | 144 | 454 | 0 |
| DW479599 | 294 | 1 | 291 | 0 |
| GO266963 | 263 | 1 | 263 | 0 |
| DW479600 | 296 | 1 | 293 | 0 |
| DT552229 | 293 | 17 | 305 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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