| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_004024m |
| Family | AA3 |
| Protein Properties | Length: 601 Molecular Weight: 65700.5 Isoelectric Point: 9.6653 |
| Chromosome | Chromosome/Scaffold: 12525 Start: 593135 End: 597224 |
| Description | Glucose-methanol-choline (GMC) oxidoreductase family protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 50 | 579 | 0 |
| GAKESAYDYIIVGGGTAGCPLAATLSQNFKVLLLERGGVPFTNPNVSFLSNFHITLADTSPTSASQCFISTDGVLNARARVLGGGTSINAGFYSRASTRF IKKVGWDERLVNESYPWIEKQIVHSPSVAPWQVALRDSLLDVGVSPFNGFTYDHIYGTKIGGTIFDRFGRRHTAAELLASGNPQMLTVLVHATVQKVLFE TSGKTPKAKGVIFKDENGKQHQAFLANNPRSEIILSCGAIGTPQMLLLSGIGPRAELEKMRIPLVLDNEFVGKEMVDNPMNTIFVPSKRPVHQSLIQTVG ITQLGVYIESSSGFGQSNDSIHCHHGIMSAEIGQLSTIPPKQRTPDAIQAYVKRKKDLPREAFQGGFILEKLANPLSTGNLSLISTNVDDNPSVTFNYFK HPKDLQQCVYGIRMAAKVVQSEYFTNFTQCDKQTLEKILNMSVIANVNLIPKHLNDTKSLEQFCKDTVITIWHYHGGCHVGKVVNPDYRVIGVERLRIVD GSTLDESPGTNPQATVLMMGRYMGLKILRD | |||
| Full Sequence |
|---|
| Protein Sequence Length: 601 Download |
| MASVGTLKLS LLCLLFFVST LSSSQGKKNL YPFIKKASSF ISSSSFSSSG AKESAYDYII 60 VGGGTAGCPL AATLSQNFKV LLLERGGVPF TNPNVSFLSN FHITLADTSP TSASQCFIST 120 DGVLNARARV LGGGTSINAG FYSRASTRFI KKVGWDERLV NESYPWIEKQ IVHSPSVAPW 180 QVALRDSLLD VGVSPFNGFT YDHIYGTKIG GTIFDRFGRR HTAAELLASG NPQMLTVLVH 240 ATVQKVLFET SGKTPKAKGV IFKDENGKQH QAFLANNPRS EIILSCGAIG TPQMLLLSGI 300 GPRAELEKMR IPLVLDNEFV GKEMVDNPMN TIFVPSKRPV HQSLIQTVGI TQLGVYIESS 360 SGFGQSNDSI HCHHGIMSAE IGQLSTIPPK QRTPDAIQAY VKRKKDLPRE AFQGGFILEK 420 LANPLSTGNL SLISTNVDDN PSVTFNYFKH PKDLQQCVYG IRMAAKVVQS EYFTNFTQCD 480 KQTLEKILNM SVIANVNLIP KHLNDTKSLE QFCKDTVITI WHYHGGCHVG KVVNPDYRVI 540 GVERLRIVDG STLDESPGTN PQATVLMMGR YMGLKILRDR LGKAAGVSGP KINQRQWDFK 600 * 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK02106 | PRK02106 | 2.0e-17 | 212 | 326 | 119 | + choline dehydrogenase; Validated | ||
| pfam05199 | GMC_oxred_C | 2.0e-26 | 424 | 569 | 151 | + GMC oxidoreductase. This domain found associated with pfam00732. | ||
| TIGR01810 | betA | 3.0e-33 | 57 | 569 | 559 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| COG2303 | BetA | 5.0e-47 | 56 | 580 | 577 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| PLN02785 | PLN02785 | 0 | 56 | 587 | 532 | + Protein HOTHEAD | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI35951.1 | 0 | 1 | 587 | 1 | 593 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002275841.1 | 0 | 31 | 587 | 28 | 584 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002327436.1 | 0 | 53 | 587 | 14 | 547 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002329018.1 | 0 | 20 | 587 | 19 | 591 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527404.1 | 0 | 31 | 586 | 41 | 596 | glucose-methanol-choline (gmc) oxidoreductase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1ju2_B | 0 | 53 | 580 | 24 | 517 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 1ju2_A | 0 | 53 | 580 | 24 | 517 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 3gdp_B | 0 | 53 | 580 | 24 | 517 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 3gdp_A | 0 | 53 | 580 | 24 | 517 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 3gdn_B | 0 | 53 | 580 | 24 | 517 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cutin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
| cutin biosynthesis | RXN-9802 | EC-1.1.1 | 16-hydroxypalmitate dehydrogenase |
| suberin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
