| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_004131m |
| Family | GT8 |
| Protein Properties | Length: 594 Molecular Weight: 69968.1 Isoelectric Point: 9.5023 |
| Chromosome | Chromosome/Scaffold: 04287 Start: 80404 End: 83295 |
| Description | plant glycogenin-like starch initiation protein 3 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 290 | 512 | 8.96831e-44 |
| KIQNNERTKRREAYVTVLHSSESYVCGAIALVQSLRKTGTKRDLILLLDKSISQPKREALAAAGWNIRLIKRIRNPLAKKYSYNEYNYSKFRLWQLTDYD KIIFIDSDIIVLRNLDVLFQFPEMTATGNDIWIFNSGIMVIEPSNCTFKILMDHRKDIISYNGGDQGFLNEVFVWWHRLPRRVNFLKNFWANTTLEASVK NELFGADPPKVYSIHYLGLKPWN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 594 Download |
| MAEGLSLQKM VKSSPSKALV IRINLIFLAF FLVIYATLLL RPSSSSVYFE NEANSLVRCS 60 FHDCHHKMET SRVIKMKAVL EEAKIHKPKP RGNTTKLEVP SFMKKMGNKV KIGMVNIDED 120 DVSEWKLHGE TIQIHFERVS ENFKWEHLFP EWIDEEEELE GPSCPEIPMP DFKAYETMDV 180 IVAKLPCKFP EKLWKREVFR LQIHLVAANL AVKKGKRDRS WKTKVVFWSR CRPMLELFRC 240 DELVRQEGDW WLYEPQMWRL EQKVSLPIGS CRLALPLWGK GIDEAYDVSK IQNNERTKRR 300 EAYVTVLHSS ESYVCGAIAL VQSLRKTGTK RDLILLLDKS ISQPKREALA AAGWNIRLIK 360 RIRNPLAKKY SYNEYNYSKF RLWQLTDYDK IIFIDSDIIV LRNLDVLFQF PEMTATGNDI 420 WIFNSGIMVI EPSNCTFKIL MDHRKDIISY NGGDQGFLNE VFVWWHRLPR RVNFLKNFWA 480 NTTLEASVKN ELFGADPPKV YSIHYLGLKP WNCYRDYDCN WNIGDQRVYA SDVAHRTWWK 540 FHDGMDENLQ KFCGLTKNRK IELDWDRMMA RKNRFNDKHW KINITDSRRK NLI* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04194 | GT8_A4GalT_like | 3.0e-11 | 385 | 512 | 163 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| PLN00176 | PLN00176 | 1.0e-11 | 300 | 462 | 202 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 2.0e-17 | 302 | 462 | 171 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 3.0e-33 | 304 | 514 | 253 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 2.0e-69 | 301 | 545 | 255 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_001154284.1 | 0 | 14 | 590 | 11 | 590 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_195059.3 | 0 | 14 | 590 | 11 | 590 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002282762.1 | 0 | 3 | 589 | 4 | 588 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002326938.1 | 0 | 234 | 593 | 1 | 360 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002510223.1 | 0 | 1 | 593 | 1 | 589 | glycogenin, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2w_B | 2e-22 | 301 | 516 | 5 | 226 | A Chain A, Crystal Structure Of The R3 Form Of Pectate Lyase A, Erwinia Chrysanthemi |
| PDB | 3u2w_A | 2e-22 | 301 | 516 | 5 | 226 | A Chain A, Crystal Structure Of The R3 Form Of Pectate Lyase A, Erwinia Chrysanthemi |
| PDB | 3qvb_A | 2e-22 | 301 | 516 | 5 | 226 | A Chain A, Crystal Structure Of The R3 Form Of Pectate Lyase A, Erwinia Chrysanthemi |
| PDB | 3q4s_A | 2e-22 | 301 | 516 | 5 | 226 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3u2x_B | 4e-22 | 301 | 516 | 5 | 226 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
