| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_004482m |
| Family | GH32 |
| Protein Properties | Length: 574 Molecular Weight: 64375.1 Isoelectric Point: 9.0018 |
| Chromosome | Chromosome/Scaffold: 12525 Start: 531768 End: 539616 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 54 | 370 | 0 |
| HFQPPKNWMNGPMIYKGLYHLFYQYNPKGAVWGNIVWGHSTSKDLVNWTPHKVAIYPSQPSDINGTWSGSATILPDGKPAILYTGINPLNQQVQNLAIPK NPSDPYLIEWVKSPNNPLMAPTPENQINASSFRDPTTAWRGVDGRWKVIVGSKINRKGLAYLYRSKDFVYWIKAKHPLHSAKNTGMWECVDFFPVSANSP VGVEMSVLGSDFKYVLKASLDDTKHDCYTIGEYDHVKDRYTPDEGSVEGDSGLRYDYGKFYASKTFFDSAKNRRLLWGWLNESSSVSDDIKKGWAGIQAI PRVVWLDKSGKQLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 574 Download |
| MDIFSMKLFF FLFALFFGYG VFELEASHHI YHTFQNLQES AVSASSQPYR TSYHFQPPKN 60 WMNGPMIYKG LYHLFYQYNP KGAVWGNIVW GHSTSKDLVN WTPHKVAIYP SQPSDINGTW 120 SGSATILPDG KPAILYTGIN PLNQQVQNLA IPKNPSDPYL IEWVKSPNNP LMAPTPENQI 180 NASSFRDPTT AWRGVDGRWK VIVGSKINRK GLAYLYRSKD FVYWIKAKHP LHSAKNTGMW 240 ECVDFFPVSA NSPVGVEMSV LGSDFKYVLK ASLDDTKHDC YTIGEYDHVK DRYTPDEGSV 300 EGDSGLRYDY GKFYASKTFF DSAKNRRLLW GWLNESSSVS DDIKKGWAGI QAIPRVVWLD 360 KSGKQLVQWP IQEIEKLRVN PVHLPSQYLK GGSVVEVPGV TASQANVEIT FKVSDFSKAE 420 VLDPSWTNPQ LLCSRKGGSV RGSLGPFGLL VLASKGMQEY TSVFFRIFKG QNKHVVLMCS 480 DQSRSSLNPD NDKTMYGAFV DVDPVHEQLS LRSLIDHSVV ESFGGHGKNC ISARVYPMLA 540 INEAAHLYAF NNGSEAVTIT RLSAWSMKKA HIN* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-53 | 43 | 537 | 520 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 3.0e-62 | 35 | 537 | 516 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-86 | 60 | 373 | 330 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-145 | 54 | 370 | 328 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 54 | 527 | 485 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAT09980.1 | 0 | 1 | 573 | 1 | 576 | cell wall apoplastic invertase [Vitis vinifera] |
| EMBL | CBI35961.1 | 0 | 18 | 573 | 18 | 574 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002279788.1 | 0 | 18 | 573 | 18 | 575 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002521396.1 | 0 | 5 | 573 | 8 | 576 | Beta-fructofuranosidase, cell wall isozyme precursor, putative [Ricinus communis] |
| RefSeq | XP_002521398.1 | 0 | 62 | 573 | 1 | 514 | Beta-fructofuranosidase, cell wall isozyme precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 46 | 573 | 5 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 46 | 573 | 1 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 46 | 573 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 46 | 573 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 46 | 573 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
