| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_004675m |
| Family | GH32 |
| Protein Properties | Length: 564 Molecular Weight: 63185.9 Isoelectric Point: 5.6129 |
| Chromosome | Chromosome/Scaffold: 07035 Start: 858738 End: 862530 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 110 | 425 | 0 |
| HFQPEKNWMNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSRDLIHWLHLPLAMVANQWYDQNGVWTGSATILPDGNIIMLYTGSTNESVQVQNLAYPA DANDPLLLEWTKYSGNPVLVPPPGIDIKDFRDPTTAWYTSEGKWRISIGSKVGKTGVALIYDTEDFINYNLKSEALHGVPGTGMWECVDFYPVSKKYQHG LDTSDNGPDVKHVVKASLDDDRHDYYAIGTYDELNSKWTPDNPDIDVGIGLRYDYGIFYASKTFYDHHKGRRVLWGWIGESDSELADVKKGWACLQAIPR TVSLDTKTGSNLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 564 Download |
| MADPNPFLPI SQPLHPTYTS LPDVSHRRFS KKLLFGKGSE QTFPSRENEN VASLSLPKET 60 AKPETLRPIS RGVSAGVSEK ASLISSGSAS SEEQYPWNNS MLSWQRTAFH FQPEKNWMNG 120 PLFYKGWYHF FYQYNPNAAV WGDIVWGHAV SRDLIHWLHL PLAMVANQWY DQNGVWTGSA 180 TILPDGNIIM LYTGSTNESV QVQNLAYPAD ANDPLLLEWT KYSGNPVLVP PPGIDIKDFR 240 DPTTAWYTSE GKWRISIGSK VGKTGVALIY DTEDFINYNL KSEALHGVPG TGMWECVDFY 300 PVSKKYQHGL DTSDNGPDVK HVVKASLDDD RHDYYAIGTY DELNSKWTPD NPDIDVGIGL 360 RYDYGIFYAS KTFYDHHKGR RVLWGWIGES DSELADVKKG WACLQAIPRT VSLDTKTGSN 420 LLQWPVEEVE NLRLRAREFK KVEVEPGSVV PLDLDAATQL DIVAVFELDK DALMKTAEST 480 EEFSCKTSRG AAHRNALGPF GLLVLADDSL AEQTPVYFYI NKGSNGTFKT FFCTDQSRSS 540 AANDVNKQIY GNLFQSLRVK NSL* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-42 | 106 | 498 | 412 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-66 | 106 | 463 | 375 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-92 | 116 | 428 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-144 | 110 | 425 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 8.0e-168 | 110 | 551 | 455 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB82419.1 | 0 | 60 | 558 | 76 | 574 | acid invertase [Citrus unshiu] |
| DDBJ | BAF34362.1 | 0 | 63 | 558 | 79 | 574 | beta-fructofuranosidase [Citrus sinensis] |
| DDBJ | BAF35859.1 | 0 | 1 | 558 | 1 | 579 | soluble acid invertase [Pyrus pyrifolia var. culta] |
| RefSeq | XP_002303519.1 | 0 | 1 | 558 | 1 | 587 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 1 | 558 | 1 | 575 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 95 | 551 | 8 | 467 | A Chain A, Arabidopsis Thaliana Peroxidase N |
| PDB | 3ugh_A | 0 | 95 | 551 | 8 | 467 | A Chain A, Arabidopsis Thaliana Peroxidase N |
| PDB | 3ugg_B | 0 | 95 | 551 | 8 | 467 | A Chain A, Arabidopsis Thaliana Peroxidase N |
| PDB | 3ugg_A | 0 | 95 | 551 | 8 | 467 | A Chain A, Arabidopsis Thaliana Peroxidase N |
| PDB | 3ugf_B | 0 | 95 | 551 | 8 | 467 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DY919082 | 296 | 130 | 425 | 0 |
| GW865424 | 279 | 149 | 427 | 0 |
| FC906419 | 289 | 119 | 407 | 0 |
| DY265807 | 298 | 119 | 416 | 0 |
| EG480171 | 267 | 95 | 358 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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