| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_004717m |
| Family | GT35 |
| Protein Properties | Length: 561 Molecular Weight: 62684.6 Isoelectric Point: 6.6201 |
| Chromosome | Chromosome/Scaffold: 12453 Start: 48 End: 4605 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 25 | 195 | 0 |
| ALTKFGHDLESVARHEPDAALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLEMGNPWEIVRNDISYPVKFYGKVVSGS DGKKHWIGGEDIMAVAYDLPIPGYKTKSTINLRLWSTKAPAEDLDLSAFNAGEQTKAYEALANAEKLISNV | |||
| GT35 | 211 | 560 | 0 |
| GEKTTAYTNHTVLPEALEKWSLDLMQNLLPRHVEIIEMIDEQLINTIVSEYGREDSDLLEKKLKEMRILENVDLPSTFANLIAKPKESSAAAISENSDEA GKLINEKDEVDSGDETESKGGPKSTGARKKEEVVAEPPPKMVRMANLCVVGGHAVNGVAAIHSEIVKDEVFNSFYKLWPNKFQNKTNGVTPRRWIRFCNP DLSKIITEWTGSEEWVLNTEKLAELRKFADNEDFQTQWRAAKKSNKMKVASLLKEKTGYSVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAV ERKAKYVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKV | |||
| Full Sequence |
|---|
| Protein Sequence Length: 561 Download |
| MEFLQGRALL NAIGNLELTG AYAEALTKFG HDLESVARHE PDAALGNGGL GRLASCFLDS 60 MATLNYPAWG YGLRYKYGLF KQRITKDGQE EVAEDWLEMG NPWEIVRNDI SYPVKFYGKV 120 VSGSDGKKHW IGGEDIMAVA YDLPIPGYKT KSTINLRLWS TKAPAEDLDL SAFNAGEQTK 180 AYEALANAEK LISNVPFCSA SVSASAWSFL GEKTTAYTNH TVLPEALEKW SLDLMQNLLP 240 RHVEIIEMID EQLINTIVSE YGREDSDLLE KKLKEMRILE NVDLPSTFAN LIAKPKESSA 300 AAISENSDEA GKLINEKDEV DSGDETESKG GPKSTGARKK EEVVAEPPPK MVRMANLCVV 360 GGHAVNGVAA IHSEIVKDEV FNSFYKLWPN KFQNKTNGVT PRRWIRFCNP DLSKIITEWT 420 GSEEWVLNTE KLAELRKFAD NEDFQTQWRA AKKSNKMKVA SLLKEKTGYS VSPDAMFDIQ 480 VKRIHEYKRQ LLNILGIVYR YKKMKEMSAV ERKAKYVPRV CIFGGKAFAT YVQAKRIVKF 540 ITDVGATVNH DPEIGDLLKV * 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 6.0e-71 | 25 | 280 | 322 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 8.0e-92 | 331 | 560 | 230 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| pfam00343 | Phosphorylase | 5.0e-92 | 332 | 560 | 229 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 3.0e-99 | 1 | 281 | 351 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 4.0e-106 | 343 | 560 | 218 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PRF/SEQDB | 0 | 1 | 560 | 132 | 763 | UP10_LACSN Unknown protein 10 from 2D-PAGE | |
| Swiss-Prot | P27598 | 0 | 1 | 560 | 132 | 763 | PHSL_IPOBA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| Swiss-Prot | P53536 | 0 | 1 | 560 | 157 | 811 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002305367.1 | 0 | 1 | 560 | 112 | 757 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 1 | 560 | 149 | 785 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2azd_B | 0 | 350 | 560 | 402 | 607 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 2azd_B | 4e-30 | 1 | 191 | 66 | 251 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 2azd_A | 0 | 350 | 560 | 402 | 607 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 2azd_A | 4e-30 | 1 | 191 | 66 | 251 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 2aw3_B | 0 | 350 | 560 | 402 | 607 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778303 | 635 | 1 | 560 | 0 |
| ES817566 | 304 | 275 | 560 | 0 |
| CO114827 | 228 | 336 | 560 | 0 |
| CO087393 | 228 | 336 | 560 | 0 |
| ES806048 | 228 | 336 | 560 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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