| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_005650m |
| Family | AA7 |
| Protein Properties | Length: 518 Molecular Weight: 57974.8 Isoelectric Point: 5.3499 |
| Chromosome | Chromosome/Scaffold: 07859 Start: 326511 End: 330180 |
| Description | cytokinin oxidase 7 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 50 | 246 | 9.9e-28 |
| MYSFNPLAIIRPAGADDVARVVKAAYRSPNMTVAARGNGHSINGQAMADGGVVIDMRSTGDNYFKIVRMNGEIFVDVSGGALWEDVLIRCVSGFNLAPRS WTDYLGLTVGGTLSNAGVSGQAFRYGPQTSNARELDVVTGKGDILTCSETENSELFFGALGGLGQFGIITRARVKLQSAPDMVRWIRLVYSEFEDFT | |||
| Full Sequence |
|---|
| Protein Sequence Length: 518 Download |
| MIAYLERFIH ENDTESIQDD RLPPICQSLD LQGSINCVAT GLAGKDFGGM YSFNPLAIIR 60 PAGADDVARV VKAAYRSPNM TVAARGNGHS INGQAMADGG VVIDMRSTGD NYFKIVRMNG 120 EIFVDVSGGA LWEDVLIRCV SGFNLAPRSW TDYLGLTVGG TLSNAGVSGQ AFRYGPQTSN 180 ARELDVVTGK GDILTCSETE NSELFFGALG GLGQFGIITR ARVKLQSAPD MVRWIRLVYS 240 EFEDFTRDAE WLVTRPDGMS FDYVEGFVFV NNDDPVNGWS SVPLDPDWGF DQTAIPPTAG 300 SVLYCLEVAL HYQNSDHPSA VDTVVNSLLG RLRFIEDLKF QVDITYVEFI LRVKQVEEHA 360 RANEIWDSPH PWLNLFVSKK DIVDFDRMVF KRLLKEGIGG PMLIYPLLRS KWDNRTSVKI 420 PDEGEIFYLV ALLRFTPPYS KGSYVEKLAS QNQEIVQLCV KNGFDFKLYL PHYHSQDDWK 480 RHFGNQWSRF VDRKASFDPM AILAPGQKIF KRVISHS* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR00387 | glcD | 0.001 | 58 | 244 | 204 | + glycolate oxidase, subunit GlcD. This protein, the glycolate oxidase GlcD subunit, is similar in sequence to that of several D-lactate dehydrogenases, including that of E. coli. The glycolate oxidase has been found to have some D-lactate dehydrogenase activity [Energy metabolism, Other]. | ||
| COG0277 | GlcD | 4.0e-22 | 48 | 507 | 483 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 2.0e-25 | 55 | 197 | 150 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 1.0e-131 | 229 | 510 | 285 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 1 | 515 | 519 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2EXR | 0 | 2 | 514 | 2 | 524 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) From Arabidopsis Thaliana At5g21482 |
| RefSeq | NP_850863.1 | 0 | 1 | 514 | 1 | 524 | CKX7 (CYTOKININ OXIDASE 7); cytokinin dehydrogenase/ oxidoreductase [Arabidopsis thaliana] |
| RefSeq | XP_002279960.1 | 0 | 1 | 512 | 1 | 511 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002309468.1 | 0 | 1 | 513 | 1 | 518 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002516133.1 | 0 | 1 | 513 | 1 | 517 | gulonolactone oxidase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 2 | 514 | 2 | 524 | A Chain A, Pectin Methylesterase Pema From Erwinia Chrysanthemi |
| PDB | 2exr_A | 0 | 2 | 514 | 2 | 524 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1d_A | 0 | 14 | 511 | 5 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 14 | 511 | 5 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 14 | 511 | 5 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO781924 | 510 | 1 | 510 | 0 |
| ES791244 | 382 | 84 | 464 | 0 |
| CF514162 | 278 | 235 | 512 | 0 |
| DV126539 | 251 | 242 | 492 | 0 |
| FC070877 | 295 | 28 | 322 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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