| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_006199m |
| Family | GH13 |
| Protein Properties | Length: 497 Molecular Weight: 57374.9 Isoelectric Point: 6.2741 |
| Chromosome | Chromosome/Scaffold: 06550 Start: 445392 End: 463520 |
| Description | starch branching enzyme 2.2 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 112 | 433 | 2e-30 |
| LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLLVLMDVVHSHASNNTLDGLNMFDGTDSHYFHSGSRGHHWLWD SRLFNYGSWEVLRFLLSNARWWLEEYRFDGFRFDGVTSMMYTHHGLQVAFTGNYNEYFGYATDVDAVIYLMLVNDMIHGLFPEAVTIGEDVSGKPTFCIP VEDGGVGFDYRLHMAIADKWIEILKKRDEDWKMGDIVHTLTNRRWLEKCVAYAESHDQALVGDKTIAFWLMDKDMYDFMARDRPSTPLIDRGIALHKMIR LITMGLGGEGYLNFMGNEFGHP | |||
| Full Sequence |
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| Protein Sequence Length: 497 Download |
| MTRNEFGVWE IFLPNNADGS PPIPHGSRVK IRMDTPSGIK DSIPAWIKFS VQAPGEIPYN 60 AIYYDPPKEE KYVFKHPQPK RPKSLRIYES HVGMSSTEPI INTYANFRDD MLPRIKKLGY 120 NAVQIMAIQE HSYYASFGYH VTNFFAPSSR FGTPDDLKSL IDKAHELGLL VLMDVVHSHA 180 SNNTLDGLNM FDGTDSHYFH SGSRGHHWLW DSRLFNYGSW EVLRFLLSNA RWWLEEYRFD 240 GFRFDGVTSM MYTHHGLQVA FTGNYNEYFG YATDVDAVIY LMLVNDMIHG LFPEAVTIGE 300 DVSGKPTFCI PVEDGGVGFD YRLHMAIADK WIEILKKRDE DWKMGDIVHT LTNRRWLEKC 360 VAYAESHDQA LVGDKTIAFW LMDKDMYDFM ARDRPSTPLI DRGIALHKMI RLITMGLGGE 420 GYLNFMGNEF GHPEWIDFPR GDRHLPNGKV IPGNNHSYDK CRRRFDLGDA DYLRYHGMQE 480 FDQAMQHLEE AYVHDF* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0296 | GlgB | 6.0e-94 | 3 | 493 | 512 | + 1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism] | ||
| PLN03244 | PLN03244 | 7.0e-123 | 21 | 490 | 474 | + alpha-amylase; Provisional | ||
| PLN02960 | PLN02960 | 4.0e-167 | 21 | 492 | 475 | + alpha-amylase | ||
| PLN02447 | PLN02447 | 0 | 1 | 492 | 496 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 69 | 483 | 416 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAT76444.1 | 0 | 1 | 492 | 234 | 725 | starch branching enzyme II [Vigna radiata] |
| GenBank | ABN05322.1 | 0 | 1 | 492 | 229 | 720 | starch branching enzyme II [Populus trichocarpa] |
| EMBL | CAA56319.1 | 0 | 1 | 492 | 250 | 741 | starch branching enzyme I [Pisum sativum] |
| RefSeq | XP_002326414.1 | 0 | 1 | 492 | 126 | 617 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002534111.1 | 0 | 1 | 492 | 242 | 733 | starch branching enzyme II, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 1 | 492 | 95 | 581 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 1 | 492 | 95 | 581 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 1 | 492 | 95 | 581 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3aml_A | 0 | 1 | 492 | 95 | 581 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3k1d_A | 9e-36 | 7 | 326 | 173 | 481 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777638 | 492 | 1 | 492 | 0 |
| HO794536 | 492 | 1 | 492 | 0 |
| HO458123 | 291 | 202 | 492 | 0 |
| HO458123 | 201 | 1 | 201 | 0 |
| HO619167 | 497 | 3 | 492 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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