| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_007592m |
| Family | GH28 |
| Protein Properties | Length: 448 Molecular Weight: 48930.5 Isoelectric Point: 7.8757 |
| Chromosome | Chromosome/Scaffold: 09856 Start: 29184 End: 35717 |
| Description | Pectin lyase-like superfamily protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH28 | 75 | 421 | 0 |
| RGGTLLYIPPGVYLTGSFNLTSHMTLYLARGAVIRATQDYSDWPLIAPLPSYGRGRERPGGRYISFIHGDGLHDVVITGENGTIDGQGDVWWNMWRQRTL QFTRPNLVEIVNSRSIIISNVIFRNSPFWNIHPVYCSHVVIRYVTILAPHDSPNTDGIDPDSSSDVCIEDSFISTGDDLVAIKSGWDEYGIAYGRPSSGI TIRRVTGSSPFAGIAVGSETSGGVENVLAENINLYNMGVGIHVKTNIGRGGFIRNITFYDVYMENVRKGIKIAGDVGDHPDENFNPNALPVVKGITLKSV WGEKVQQPGSIQGLKNSPFTGICLSNINLHGVPGPRSTPWKCSAVRG | |||
| Full Sequence |
|---|
| Protein Sequence Length: 448 Download |
| MSLHFLSLFL GLSVFVLFSG HFAVAGVETC SGIVSMRYRY DKISITDFGG VGDGRTLNTK 60 AFREAIYRIQ HLRRRGGTLL YIPPGVYLTG SFNLTSHMTL YLARGAVIRA TQDYSDWPLI 120 APLPSYGRGR ERPGGRYISF IHGDGLHDVV ITGENGTIDG QGDVWWNMWR QRTLQFTRPN 180 LVEIVNSRSI IISNVIFRNS PFWNIHPVYC SHVVIRYVTI LAPHDSPNTD GIDPDSSSDV 240 CIEDSFISTG DDLVAIKSGW DEYGIAYGRP SSGITIRRVT GSSPFAGIAV GSETSGGVEN 300 VLAENINLYN MGVGIHVKTN IGRGGFIRNI TFYDVYMENV RKGIKIAGDV GDHPDENFNP 360 NALPVVKGIT LKSVWGEKVQ QPGSIQGLKN SPFTGICLSN INLHGVPGPR STPWKCSAVR 420 GAAHEVSPWP CSELTATHQT ASCSDHI* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02188 | PLN02188 | 4.0e-20 | 43 | 431 | 413 | + polygalacturonase/glycoside hydrolase family protein | ||
| PLN03003 | PLN03003 | 9.0e-24 | 41 | 346 | 312 | + Probable polygalacturonase At3g15720 | ||
| PLN02218 | PLN02218 | 3.0e-24 | 43 | 405 | 377 | + polygalacturonase ADPG | ||
| pfam00295 | Glyco_hydro_28 | 1.0e-28 | 140 | 419 | 286 | + Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes is important in cell wall metabolism. | ||
| COG5434 | PGU1 | 3.0e-55 | 44 | 409 | 400 | + Endopygalactorunase [Cell envelope biogenesis, outer membrane] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004650 | polygalacturonase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACC91250.1 | 0 | 6 | 444 | 6 | 441 | glycoside hydrolase family 28 protein [Arabidopsis halleri] |
| EMBL | CBI34471.1 | 0 | 1 | 447 | 1 | 447 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_194113.1 | 0 | 10 | 444 | 4 | 441 | glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein [Arabidopsis thaliana] |
| RefSeq | XP_002304552.1 | 0 | 36 | 446 | 1 | 411 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002509949.1 | 0 | 12 | 447 | 13 | 446 | Polygalacturonase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3jur_D | 3.00018e-42 | 42 | 405 | 27 | 412 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 3jur_C | 3.00018e-42 | 42 | 405 | 27 | 412 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 3jur_B | 3.00018e-42 | 42 | 405 | 27 | 412 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 3jur_A | 3.00018e-42 | 42 | 405 | 27 | 412 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 2uvf_B | 8e-22 | 43 | 428 | 157 | 605 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| homogalacturonan degradation | RXN-2103 | EC-3.2.1.15 | polygalacturonase |
