| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_007721m |
| Family | GT21 |
| Protein Properties | Length: 444 Molecular Weight: 50048 Isoelectric Point: 6.937 |
| Chromosome | Chromosome/Scaffold: 09501 Start: 388807 End: 394050 |
| Description | Nucleotide-diphospho-sugar transferases superfamily protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT21 | 84 | 258 | 3e-34 |
| PRVTVVMPLKGFGEHNLHNWRSQITSLYGGPLEFLFVVESTEDPAYRAVSRIIAEFKDDVDATVIVAGLSTTCSQKIHNQLFGVEKMHKDSKYVLFLDDD VRLHPGSIGALTAEMEKNPEIFIQTGYPLDLPSGSLGSYCIYEYHMPCSMGFATGGKTFFLWGGCMMMHADDFRH | |||
| Full Sequence |
|---|
| Protein Sequence Length: 444 Download |
| MPVLDSLDLF LFSLSRSFCS PLAVFFQIQG CVICLTLAIG WAFAAFIRNR EIKLMKSSMR 60 AGNSFAFLFH DINELEHSNQ VNLPRVTVVM PLKGFGEHNL HNWRSQITSL YGGPLEFLFV 120 VESTEDPAYR AVSRIIAEFK DDVDATVIVA GLSTTCSQKI HNQLFGVEKM HKDSKYVLFL 180 DDDVRLHPGS IGALTAEMEK NPEIFIQTGY PLDLPSGSLG SYCIYEYHMP CSMGFATGGK 240 TFFLWGGCMM MHADDFRHDR YGVVSGLRDG GYSDDMTLAA IAGAHKRLIT SPPVAVFPHP 300 LASDLSFSRY WNYLRKQTFV LESYTTKVNW LMNRALFSSH CYLSWGFVAP YFMSMTHVAA 360 ALRIYIRGYA HEETIFVSKG LLLVTCLAVC TFIELFSMWN LTRVEVQLCN MLSPEAPRLS 420 LASYNWVLVS ISSYLVQLHF VWN* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR03472 | HpnI | 5.0e-6 | 84 | 200 | 117 | + hopanoid biosynthesis associated glycosyl transferase protein HpnI. This family of genes include a glycosyl transferase, group 2 domain (pfam00535) which are responsible, generally for the transfer of nucleotide-diphosphate sugars to substrates such as polysaccharides and lipids. The member of this clade from Acidithiobacillus ferrooxidans ATCC 23270 (AFE_0974) is found in the same locus as squalene-hopene cyclase (SHC, TIGR01507) and other genes associated with the biosynthesis of hopanoid natural products. Similarly, in Ralstonia eutropha JMP134 (Reut_B4902) this gene is adjacent to HpnAB, IspH and HpnH (TIGR03470), although SHC itself is elsewhere in the genome. Notably, this gene (here named HpnI) and three others form a conserved set (HpnIJKL) which occur in a subset of all genomes containing the SHC enzyme. This relationship was discerned using the method of partial phylogenetic profiling. This group includes Zymomonas mobilis, the organism where the initial hopanoid biosynthesis locus was described consisting of the genes HpnA-E and SHC (HpnF). Continuing past SHC are found a phosphorylase enzyme (ZMO0873, i.e. HpnG, TIGR03468) and another radical SAM enzyme (ZMO0874), HpnH. Although discontinuous in Z. mobilis, we continue the gene symbol sequence with HpnIJKL. Hopanoids are known to feature polar glycosyl head groups in many organisms. | ||
| pfam13641 | Glyco_tranf_2_3 | 1.0e-6 | 84 | 257 | 181 | + Glycosyltransferase like family 2. Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis. | ||
| COG1215 | COG1215 | 2.0e-9 | 79 | 440 | 379 | + Glycosyltransferases, probably involved in cell wall biogenesis [Cell envelope biogenesis, outer membrane] | ||
| cd02520 | Glucosylceramide_synthase | 5.0e-10 | 84 | 315 | 237 | + Glucosylceramide synthase catalyzes the first glycosylation step of glycosphingolipid synthesis. UDP-glucose:N-acylsphingosine D-glucosyltransferase (glucosylceramide synthase or ceramide glucosyltransferase) catalyzes the first glycosylation step of glycosphingolipid synthesis. Its product, glucosylceramide, serves as the core of more than 300 glycosphingolipids (GSL). GSLs are a group of membrane components that have the lipid portion embedded in the outer plasma membrane leaflet and the sugar chains extended to the outer environment. Several lines of evidence suggest the importance of GSLs in various cellular processes such as differentiation, adhesion, proliferation, and cell-cell recognition. In pathogenic fungus Cryptococcus neoformans, glucosylceramide serves as an antigen that elicits an antibody response in patients and it is essential for fungal growth in host extracellular environment. | ||
| pfam13506 | Glyco_transf_21 | 6.0e-11 | 143 | 315 | 177 | + Glycosyl transferase family 21. This is a family of ceramide beta-glucosyltransferases - EC:2.4.1.80. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAK73021.1 | 0 | 3 | 432 | 4 | 433 | AF367245_1 ceramide glucosyltransferase [Gossypium arboreum] |
| GenBank | ABK95617.1 | 0 | 5 | 432 | 3 | 430 | unknown [Populus trichocarpa] |
| RefSeq | XP_002325028.1 | 0 | 5 | 432 | 3 | 420 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002329645.1 | 0 | 10 | 432 | 11 | 439 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002514215.1 | 0 | 3 | 432 | 6 | 435 | ceramide glucosyltransferase, putative [Ricinus communis] |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sphingolipid biosynthesis (plants) | RXN-7793 | EC-2.4.1.80 | ceramide glucosyltransferase |
