| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_008801m |
| Family | AA7 |
| Protein Properties | Length: 409 Molecular Weight: 45043.1 Isoelectric Point: 4.935 |
| Chromosome | Chromosome/Scaffold: 04056 Start: 11925 End: 13441 |
| Description | cytokinin oxidase 7 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 52 | 243 | 2e-31 |
| SFNPLAIIRPAGADDIAMAVKAACRSPNLTVAARGNGHSINGQAMAEGGLVIDMRATGENHFKIVTMNGEMFADVSGGALWEDVLKRCVNLAPRSWTDYL GLTVGGTLSNAGVSGQAFRYGPQSSNVTELDVVTGKGDILTCSETENSELFFGALGGLGQFGIITRARVKLQSTPDMVRWIRVVYSEFEDFT | |||
| Full Sequence |
|---|
| Protein Sequence Length: 409 Download |
| MIAYIERFIH ENDTESRQGD DLSNICQSLD LQGTIDYVAT GIAAKDFGGL YSFNPLAIIR 60 PAGADDIAMA VKAACRSPNL TVAARGNGHS INGQAMAEGG LVIDMRATGE NHFKIVTMNG 120 EMFADVSGGA LWEDVLKRCV NLAPRSWTDY LGLTVGGTLS NAGVSGQAFR YGPQSSNVTE 180 LDVVTGKGDI LTCSETENSE LFFGALGGLG QFGIITRARV KLQSTPDMVR WIRVVYSEFE 240 DFTHDAEWLV NRPDGVSFDY VEGFVFVNSD DPVNGWCTVP LDPSQRFDQT AIPPTAGYVL 300 YCLEVALHYQ NSDHRSAVDM VVNSLLGRLR FAKDLKFQVD VAYVEFLLRL KRVEEHARAN 360 GTWDSPHPWL NMFISKRDIA DFDRMVFKKL LKEGVGGPML VYPLLRSK* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02805 | PLN02805 | 0.003 | 20 | 226 | 220 | + D-lactate dehydrogenase [cytochrome] | ||
| COG0277 | GlcD | 7.0e-19 | 35 | 252 | 231 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 1.0e-25 | 55 | 194 | 144 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 1.0e-76 | 226 | 408 | 186 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 1 | 408 | 414 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABK32520.1 | 0 | 1 | 408 | 1 | 405 | cytokinin oxidase/dehydrogenase 1 [Pisum sativum] |
| RefSeq | NP_850863.1 | 0 | 1 | 408 | 1 | 421 | CKX7 (CYTOKININ OXIDASE 7); cytokinin dehydrogenase/ oxidoreductase [Arabidopsis thaliana] |
| RefSeq | XP_002279960.1 | 0 | 1 | 408 | 1 | 411 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002309468.1 | 0 | 1 | 408 | 1 | 416 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002516133.1 | 0 | 1 | 408 | 1 | 415 | gulonolactone oxidase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 2 | 408 | 2 | 421 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 2exr_A | 0 | 2 | 408 | 2 | 421 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1d_A | 0 | 28 | 407 | 24 | 418 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3kjm_A | 0 | 28 | 407 | 24 | 418 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 2qpm_A | 0 | 28 | 407 | 24 | 418 | A Chain A, Leu492ala Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With Benzylurea Inhibitor Cpbu |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO781924 | 411 | 1 | 408 | 0 |
| ES791244 | 318 | 84 | 398 | 0 |
| FC070877 | 296 | 28 | 320 | 0 |
| GR446435 | 278 | 44 | 318 | 0 |
| EG505655 | 308 | 19 | 318 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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