| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_009211m |
| Family | GT35 |
| Protein Properties | Length: 395 Molecular Weight: 44019 Isoelectric Point: 5.389 |
| Chromosome | Chromosome/Scaffold: 12453 Start: 419 End: 4605 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 113 | 383 | 0 |
| GEKTTAYTNHTVLPEALEKWSLDLMQNLLPRHVEIIEMIDEQLINTIVSEYGREDSDLLEKKLKEMRILENVDLPSTFANLIAKPKESSAAAISENSDEA GKLINEKDEVDSGDETESKGGPKSTGARKKEEVVAEPPPKMVRMANLCVVGGHAVNGVAAIHSEIVKDEVFNSFYKLWPNKFQNKTNGVTPRRWIRFCNP DLSKIITEWTGSEEWVLNTEKLAELRKFADNEDFQTQWRAAKKSNKMKVASLLKEKTGYSVSPDAMFDIQV | |||
| Full Sequence |
|---|
| Protein Sequence Length: 395 Download |
| MGNPWEIVRN DISYPVKFYG KVVSGSDGKK HWIGGEDIMA VAYDLPIPGY KTKSTINLRL 60 WSTKAPAEDL DLSAFNAGEQ TKAYEALANA EKLISNVPFC SASVSASAWS FLGEKTTAYT 120 NHTVLPEALE KWSLDLMQNL LPRHVEIIEM IDEQLINTIV SEYGREDSDL LEKKLKEMRI 180 LENVDLPSTF ANLIAKPKES SAAAISENSD EAGKLINEKD EVDSGDETES KGGPKSTGAR 240 KKEEVVAEPP PKMVRMANLC VVGGHAVNGV AAIHSEIVKD EVFNSFYKLW PNKFQNKTNG 300 VTPRRWIRFC NPDLSKIITE WTGSEEWVLN TEKLAELRKF ADNEDFQTQW RAAKKSNKMK 360 VASLLKEKTG YSVSPDAMFD IQVHLLFLKS SYSC* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 8.0e-40 | 2 | 182 | 247 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| pfam00343 | Phosphorylase | 1.0e-49 | 234 | 383 | 150 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 7.0e-54 | 2 | 183 | 251 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 2.0e-60 | 245 | 383 | 139 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 2.0e-79 | 2 | 383 | 429 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PRF/SEQDB | 0 | 1 | 383 | 230 | 684 | UP10_LACSN Unknown protein 10 from 2D-PAGE | |
| Swiss-Prot | P27598 | 0 | 1 | 383 | 230 | 684 | PHSL_IPOBA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| Swiss-Prot | P53536 | 0 | 1 | 383 | 255 | 732 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002305367.1 | 0 | 1 | 383 | 210 | 678 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 1 | 383 | 247 | 706 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2azd_B | 3e-39 | 252 | 386 | 402 | 535 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2azd_B | 0.00000003 | 108 | 155 | 332 | 378 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2azd_B | 0.001 | 4 | 93 | 167 | 251 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2azd_A | 3e-39 | 252 | 386 | 402 | 535 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2azd_A | 0.00000003 | 108 | 155 | 332 | 378 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778303 | 458 | 1 | 383 | 0 |
| DW128204 | 277 | 108 | 383 | 0 |
| CO123548 | 279 | 108 | 375 | 0 |
| CK650229 | 178 | 193 | 370 | 0 |
| GR191353 | 254 | 130 | 383 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
