| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_009867m |
| Family | AA2 |
| Protein Properties | Length: 376 Molecular Weight: 40667.9 Isoelectric Point: 7.9967 |
| Chromosome | Chromosome/Scaffold: 04314 Start: 122027 End: 127581 |
| Description | stromal ascorbate peroxidase |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA2 | 108 | 353 | 0 |
| LLKSKFCHPILVRLGWHDAGTYNKNIEEWPKRGGADGSIRFEVELKHGANAGLVNALNLLQPIKDKYSGVTYADLFQLASATAIEEAGGPKIPMKYGRVD VSAPEDCPEEGRLPSAGPPKPADHLREVFYRMGLNDQEIVALSGAHTLGRSRPDRSGWGKPETKYTKSGPGAPGGQSWTAEWLKFDNSYFTDIKERRDED LLVLPTDAVIFEDSSFKVYAEKYAADQEAFFKDYAEAHAKLSNLGA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 376 Download |
| MADCLSHLTL SPSQKTMASL TGAASSRLLP AASRARIHLS SPSSASSSRS PLSLSSSSSY 60 SISPLKCLRS STFVPHFFLN QKRSSMSTVA AASDPVQLKS AREDIKELLK SKFCHPILVR 120 LGWHDAGTYN KNIEEWPKRG GADGSIRFEV ELKHGANAGL VNALNLLQPI KDKYSGVTYA 180 DLFQLASATA IEEAGGPKIP MKYGRVDVSA PEDCPEEGRL PSAGPPKPAD HLREVFYRMG 240 LNDQEIVALS GAHTLGRSRP DRSGWGKPET KYTKSGPGAP GGQSWTAEWL KFDNSYFTDI 300 KERRDEDLLV LPTDAVIFED SSFKVYAEKY AADQEAFFKD YAEAHAKLSN LGAKFDPPQG 360 IVLDGVAGEK FVAAN* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00314 | plant_peroxidase_like | 2.0e-41 | 118 | 350 | 254 | + Heme-dependent peroxidases similar to plant peroxidases. Along with animal peroxidases, these enzymes belong to a group of peroxidases containing a heme prosthetic group (ferriprotoporphyrin IX), which catalyzes a multistep oxidative reaction involving hydrogen peroxide as the electron acceptor. The plant peroxidase-like superfamily is found in all three kingdoms of life and carries out a variety of biosynthetic and degradative functions. Several sub-families can be identified. Class I includes intracellular peroxidases present in fungi, plants, archaea and bacteria, called catalase-peroxidases, that can exhibit both catalase and broad-spectrum peroxidase activities depending on the steady-state concentration of hydrogen peroxide. Catalase-peroxidases are typically comprised of two homologous domains that probably arose via a single gene duplication event. Class II includes ligninase and other extracellular fungal peroxidases, while class III is comprised of classic extracellular plant peroxidases, like horseradish peroxidase. | ||
| PLN02364 | PLN02364 | 7.0e-57 | 98 | 352 | 256 | + L-ascorbate peroxidase 1 | ||
| PLN02879 | PLN02879 | 2.0e-60 | 98 | 352 | 255 | + L-ascorbate peroxidase | ||
| PLN02608 | PLN02608 | 5.0e-87 | 97 | 358 | 262 | + L-ascorbate peroxidase | ||
| cd00691 | ascorbate_peroxidase | 1.0e-135 | 86 | 356 | 275 | + Ascorbate peroxidases and cytochrome C peroxidases. Ascorbate peroxidases are a subgroup of heme-dependent peroxidases of the plant superfamily that share a heme prosthetic group and catalyze a multistep oxidative reaction involving hydrogen peroxide as the electron acceptor. Along with related catalase-peroxidases, ascorbate peroxidases belong to class I of the plant superfamily. Ascorbate peroxidases are found in the chloroplasts and/or cytosol of algae and plants, where they have been shown to control the concentration of lethal hydrogen peroxide molecules. The yeast cytochrome c peroxidase is a divergent member of the family; it forms a complex with cytochrome c to catalyze the reduction of hydrogen peroxide to water. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004601 | peroxidase activity |
| GO:0006979 | response to oxidative stress |
| GO:0020037 | heme binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC19394.1 | 0 | 67 | 374 | 53 | 373 | stromal L-ascorbate peroxidase precursor [Mesembryanthemum crystallinum] |
| GenBank | AAS55852.1 | 0 | 17 | 374 | 1 | 357 | chloroplast thylakoid-bound ascorbate peroxidase [Vigna unguiculata] |
| GenBank | AAS55853.1 | 0 | 17 | 374 | 1 | 357 | chloroplast stromal ascorbate peroxidase [Vigna unguiculata] |
| RefSeq | XP_002300978.1 | 0 | 17 | 360 | 1 | 339 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002520353.1 | 0 | 1 | 374 | 1 | 372 | Cytochrome c peroxidase, mitochondrial precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1iyn_A | 0 | 91 | 374 | 1 | 288 | A Chain A, Crystal Structure Of Chloroplastic Ascorbate Peroxidase From Tobacco Plants And Structural Insights For Its Instability |
| PDB | 1apx_D | 0 | 98 | 352 | 15 | 245 | A Chain A, Crystal Structure Of Recombinant Ascorbate Peroxidase |
| PDB | 1apx_C | 0 | 98 | 352 | 15 | 245 | A Chain A, Crystal Structure Of Recombinant Ascorbate Peroxidase |
| PDB | 1apx_B | 0 | 98 | 352 | 15 | 245 | A Chain A, Crystal Structure Of Recombinant Ascorbate Peroxidase |
| PDB | 1apx_A | 0 | 98 | 352 | 15 | 245 | A Chain A, Crystal Structure Of Recombinant Ascorbate Peroxidase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| L-ascorbate degradation III | RXN-12440 | EC-1.11.1.11 | L-ascorbate peroxidase |
| L-ascorbate degradation V | RXN-12440 | EC-1.11.1.11 | L-ascorbate peroxidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO779395 | 323 | 66 | 374 | 0 |
| EX262979 | 305 | 78 | 374 | 0 |
| GO599900 | 288 | 87 | 374 | 0 |
| DT549779 | 263 | 84 | 346 | 0 |
| GT000953 | 280 | 100 | 375 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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