| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_011328m |
| Family | GT2 |
| Protein Properties | Length: 338 Molecular Weight: 38258.5 Isoelectric Point: 9.6946 |
| Chromosome | Chromosome/Scaffold: 08265 Start: 1874056 End: 1879607 |
| Description | Nucleotide-diphospho-sugar transferases superfamily protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT2 | 71 | 267 | 2.1e-32 |
| SLIVPAFNEEHRLPAALDETMNYLQQRAAKDKSFTYEVVIVDDGSSDATKRVAFGFVKKYTVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGA TKVDDLEKLENQIHAVAKREFKLGDGADTYSNSRISDFPIAAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRAAARK | |||
| Full Sequence |
|---|
| Protein Sequence Length: 338 Download |
| MGLGFLWTAA ERLVALALLI PTLLLFQVIF EAYKRRHHNK HVNAPAIFED PNSLKQVPCP 60 HVNDPAEKYI SLIVPAFNEE HRLPAALDET MNYLQQRAAK DKSFTYEVVI VDDGSSDATK 120 RVAFGFVKKY TVDNVRVILL GRNHGKGEAI RKGMLHSRGE LLLMLDADGA TKVDDLEKLE 180 NQIHAVAKRE FKLGDGADTY SNSRISDFPI AAFGSRAHLE EKALATRKWY RNFLMKGFHL 240 VVLLTAGPGI RDTQCGFKMF TRAAARKLFT NIRLKRWCFD VEVVYLCKRF NIPIIEISVN 300 WSEIPGSKVN LLSIPNMLWE LAIMSLGYRT GLWEIQK* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04187 | DPM1_like_bac | 2.0e-19 | 72 | 268 | 197 | + Bacterial DPM1_like enzymes are related to eukaryotic DPM1. A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily. | ||
| pfam00535 | Glycos_transf_2 | 3.0e-23 | 71 | 269 | 199 | + Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids. | ||
| cd04179 | DPM_DPG-synthase_like | 3.0e-48 | 72 | 287 | 216 | + DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily. DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily. | ||
| PTZ00260 | PTZ00260 | 5.0e-94 | 66 | 336 | 275 | + dolichyl-phosphate beta-glucosyltransferase; Provisional | ||
| cd04188 | DPG_synthase | 4.0e-102 | 72 | 308 | 237 | + DPG_synthase is involved in protein N-linked glycosylation. UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_181493.1 | 0 | 34 | 337 | 32 | 335 | glycosyl transferase family 2 protein [Arabidopsis thaliana] |
| RefSeq | XP_002283615.1 | 0 | 32 | 336 | 30 | 335 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002312095.1 | 0 | 32 | 335 | 30 | 333 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002315224.1 | 0 | 33 | 336 | 31 | 334 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002530857.1 | 0 | 34 | 337 | 32 | 335 | Dolichyl-phosphate beta-glucosyltransferase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3o3p_B | 0.0007 | 70 | 168 | 96 | 189 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3o3p_A | 0.0007 | 70 | 168 | 96 | 189 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3kia_C | 0.0007 | 70 | 168 | 96 | 189 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3kia_A | 0.0007 | 70 | 168 | 96 | 189 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3f1y_C | 0.0007 | 70 | 168 | 96 | 189 | A Chain A, Mannosyl-3-Phosphoglycerate Synthase From Rubrobacter Xylanophilus |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| dolichyl-diphosphooligosaccharide biosynthesis | 2.4.1.117-RXN | EC-2.4.1.117 | dolichyl-phosphate β-glucosyltransferase |
