| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_011889m |
| Family | CE10 |
| Protein Properties | Length: 323 Molecular Weight: 35827.8 Isoelectric Point: 6.6495 |
| Chromosome | Chromosome/Scaffold: 05162 Start: 613959 End: 615091 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 38 | 320 | 0 |
| DPETGVFSKDTVIVPETRVSARLYRPSLAKASQKLPLVIYFHGGGFFISSTADPLYHRSLNKLVAEANIVLVSVDYRLAPENSLPAAYEDSWAALNWVAA HAGEGDGKEAWLKDYADFGRVFLGGDSCGANMTHHLTLKLKGSELSRELKIEGVAMIHPYFWGQDPVGVEKIDHVRRSMVDNWWTFICPSHKGCDDPLIN PFADGSPSLEGLACNRVLVIVADKDILRDRGKLYYEKLLSSGWKGTAKLVEIRGEDHVFHIFDPNTENAKSMFRDLASFFGKA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 323 Download |
| MVSEKAQVSH DVFPYLRVYK DGTIERFAGT EVVPAGLDPE TGVFSKDTVI VPETRVSARL 60 YRPSLAKASQ KLPLVIYFHG GGFFISSTAD PLYHRSLNKL VAEANIVLVS VDYRLAPENS 120 LPAAYEDSWA ALNWVAAHAG EGDGKEAWLK DYADFGRVFL GGDSCGANMT HHLTLKLKGS 180 ELSRELKIEG VAMIHPYFWG QDPVGVEKID HVRRSMVDNW WTFICPSHKG CDDPLINPFA 240 DGSPSLEGLA CNRVLVIVAD KDILRDRGKL YYEKLLSSGW KGTAKLVEIR GEDHVFHIFD 300 PNTENAKSMF RDLASFFGKA RH* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 2.0e-10 | 60 | 186 | 141 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| PRK10162 | PRK10162 | 1.0e-10 | 48 | 204 | 160 | + acetyl esterase; Provisional | ||
| pfam00135 | COesterase | 3.0e-12 | 61 | 186 | 140 | + Carboxylesterase family. | ||
| COG0657 | Aes | 6.0e-33 | 30 | 320 | 298 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 2.0e-54 | 75 | 298 | 228 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABB89003.1 | 0 | 3 | 319 | 4 | 315 | CXE carboxylesterase [Malus pumila] |
| RefSeq | XP_002284585.1 | 0 | 1 | 317 | 1 | 317 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002327124.1 | 0 | 2 | 320 | 1 | 319 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002510251.1 | 0 | 2 | 320 | 1 | 312 | Gibberellin receptor GID1, putative [Ricinus communis] |
| RefSeq | XP_002526230.1 | 0 | 2 | 320 | 1 | 320 | catalytic, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2zsi_A | 2e-36 | 21 | 316 | 45 | 347 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2zsh_A | 2e-36 | 21 | 316 | 45 | 347 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2o7v_A | 7e-36 | 21 | 316 | 29 | 326 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2o7r_A | 7e-36 | 21 | 316 | 29 | 326 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_F | 1e-31 | 21 | 317 | 37 | 347 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DV447885 | 242 | 82 | 323 | 0 |
| ES789964 | 317 | 3 | 319 | 0 |
| EG631244 | 317 | 3 | 319 | 0 |
| DY306514 | 318 | 4 | 311 | 0 |
| DY288581 | 314 | 4 | 307 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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