| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_012170m |
| Family | GH19 |
| Protein Properties | Length: 316 Molecular Weight: 34031.4 Isoelectric Point: 7.6266 |
| Chromosome | Chromosome/Scaffold: 02959 Start: 47595 End: 49925 |
| Description | basic chitinase |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH19 | 65 | 298 | 0 |
| DLSSIISRSTFDQMLKHRNDGGCSAKGFYTYDAFISAAKAFPSFGNTGDVAVRKREIAAFFAQTSHETTGWATAPDGPYAWGYCFLKEQNPGSYCSPSPT YPCAPGKQYYGRGPIQLSWNYNYGQCGRAIGIDLLNNPDLVATDPVISFKTAIWFWMTPQSPKPSCHDVIIGRWSPSQADRSAGRVPGYGTVTNIINGGL ECGKGWNAKVEDRIGFYKRYCDLLGVGYGSNLDC | |||
| Full Sequence |
|---|
| Protein Sequence Length: 316 Download |
| MRALAFAVSC LLLSHLLEAS AEQCGRQAGG ALCPGGLCCS KFGWCGNTPD YCGADCQSQC 60 SAGKDLSSII SRSTFDQMLK HRNDGGCSAK GFYTYDAFIS AAKAFPSFGN TGDVAVRKRE 120 IAAFFAQTSH ETTGWATAPD GPYAWGYCFL KEQNPGSYCS PSPTYPCAPG KQYYGRGPIQ 180 LSWNYNYGQC GRAIGIDLLN NPDLVATDPV ISFKTAIWFW MTPQSPKPSC HDVIIGRWSP 240 SQADRSAGRV PGYGTVTNII NGGLECGKGW NAKVEDRIGF YKRYCDLLGV GYGSNLDCYN 300 QRPFGNGLLD LVDAM* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06921 | ChtBD1_GH19_hevein | 1.0e-7 | 23 | 61 | 39 | + Hevein or Type 1 chitin binding domain subfamily co-occuring with family 19 glycosyl hydrolases or with barwin domains. This subfamily includes Hevein, a major IgE-binding allergen in natural rubber latex. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements. | ||
| COG3179 | COG3179 | 9.0e-11 | 77 | 264 | 210 | + Predicted chitinase [General function prediction only] | ||
| cd00442 | lysozyme_like | 3.0e-16 | 122 | 260 | 140 | + lysozyme_like domain. This contains several members including Soluble Lytic Transglycosylases (SLT), Goose Egg-White Lysozymes (GEWL), Hen Egg-White Lysozymes (HEWL), chitinases, bacteriophage lambda lysozymes, endolysins, autolysins, and chitosanases. All the members are involved in the hydrolysis of beta-1,4- linked polysaccharides. | ||
| cd00325 | chitinase_glyco_hydro_19 | 3.0e-147 | 70 | 298 | 230 | + Glycoside hydrolase family 19 chitinase domain. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Family 19 chitinases are found primarily in plants (classes I, III, and IV), but some are found in bacteria. Class I and II chitinases are similar in their catalytic domains. Class I chitinases have an N-terminal cysteine-rich, chitin-binding domain which is separated from the catalytic domain by a proline and glycine-rich hinge region. Class II chitinases lack both the chitin-binding domain and the hinge region. Class IV chitinases are similar to class I chitinases but they are smaller in size due to certain deletions. Despite any significant sequence homology with lysozymes, structural analysis reveals that family 19 chitinases, together with family 46 chitosanases, are similar to several lysozymes including those from T4-phage and from goose. The structures reveal that the different enzyme groups arose from a common ancestor glycohydrolase antecedent to the procaryotic/eucaryotic divergence. | ||
| pfam00182 | Glyco_hydro_19 | 8.0e-154 | 69 | 298 | 232 | + Chitinase class I. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004568 | chitinase activity |
| GO:0006032 | chitin catabolic process |
| GO:0008061 | chitin binding |
| GO:0016998 | cell wall macromolecule catabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC16011.1 | 0 | 3 | 315 | 5 | 317 | basic chitinase [Elaeagnus umbellata] |
| GenBank | ABK96628.1 | 0 | 1 | 315 | 1 | 318 | unknown [Populus trichocarpa x Populus deltoides] |
| RefSeq | XP_002306220.1 | 0 | 21 | 315 | 21 | 321 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002312923.1 | 0 | 1 | 315 | 1 | 318 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002525743.1 | 0 | 1 | 315 | 5 | 325 | class I chitinase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3cql_B | 0 | 66 | 305 | 2 | 243 | A Chain A, Crystal Structure Of Gh Family 19 Chitinase From Carica Papaya |
| PDB | 3cql_A | 0 | 66 | 305 | 2 | 243 | A Chain A, Crystal Structure Of Gh Family 19 Chitinase From Carica Papaya |
| PDB | 3iwr_B | 0 | 22 | 307 | 2 | 301 | A Chain A, Crystal Structure Of Gh Family 19 Chitinase From Carica Papaya |
| PDB | 3iwr_A | 0 | 22 | 307 | 2 | 301 | A Chain A, Crystal Structure Of Gh Family 19 Chitinase From Carica Papaya |
| PDB | 2dkv_A | 0 | 22 | 307 | 2 | 301 | A Chain A, Crystal Structure Of Class I Chitinase From Oryza Sativa L. Japonica |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| chitin degradation II | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12623 | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12624 | EC-3.2.1.14 | chitinase |
| chitin degradation III (carnivorous plants) | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
