| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_021281m |
| Family | GT20 |
| Protein Properties | Length: 920 Molecular Weight: 104049 Isoelectric Point: 7.0377 |
| Chromosome | Chromosome/Scaffold: 09120 Start: 481585 End: 490676 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 92 | 559 | 0 |
| KQRLLVVANSLPVSAIRKGQDSWKLEISVDGLVNPLLGVKEFEARWIGWAGVNVPDEIGQKALSTALAEKKCIPVFLDEHIVHQYYNGYCNNVLWPLFHY LGLPQEDRLPTTRSFQSQFDAYKKANQMFADVVNRHYEEGDVVWCHDYHLMFLPKCLKEYNSKMKVGWFLHTPFPSSEIHRMLPSRSELLRSVLAADLIG FHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRVAAFPIGIDSDRFIRALELPQVQDHIKELKDKFSGRKVMLGVDRLDMIKGIPRKILAFEEFLEEN PEWRDKVVLLQIAVPSRTHVPEHQKLTSQVHKIVTRINGRFGTLTAVPIHHLDRTLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQTSKKGVLIL SEFAGAAQSLGAGAILVNPWNITEVASSISYALNMPADERENRHNHNFRHVTTHTSQEWAATFVSELN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 920 Download |
| MISKQLLTER YAPCQGLDVM SCKLQASSQN KNNCTLVTHK TGLESLLLDR VRKFTELFAN 60 DSVTKETETH GPLHEDTSLE EVNVCERPRR PKQRLLVVAN SLPVSAIRKG QDSWKLEISV 120 DGLVNPLLGV KEFEARWIGW AGVNVPDEIG QKALSTALAE KKCIPVFLDE HIVHQYYNGY 180 CNNVLWPLFH YLGLPQEDRL PTTRSFQSQF DAYKKANQMF ADVVNRHYEE GDVVWCHDYH 240 LMFLPKCLKE YNSKMKVGWF LHTPFPSSEI HRMLPSRSEL LRSVLAADLI GFHTYDYARH 300 FVSACTRILG LEGTPEGVED QGKLTRVAAF PIGIDSDRFI RALELPQVQD HIKELKDKFS 360 GRKVMLGVDR LDMIKGIPRK ILAFEEFLEE NPEWRDKVVL LQIAVPSRTH VPEHQKLTSQ 420 VHKIVTRING RFGTLTAVPI HHLDRTLDFH ALCALYAVTD VALVTSLRDG MNLVSYEFVA 480 CQTSKKGVLI LSEFAGAAQS LGAGAILVNP WNITEVASSI SYALNMPADE RENRHNHNFR 540 HVTTHTSQEW AATFVSELND TIVEAQLRTR QVLPLLPIHD AVERYLQSNN RLLILGFNAT 600 LTEPVDSLGR RSGQIREMEP RLHPGLKEPL KKLCDDQMTT IVVLSGSDRT ILDENFGGYN 660 MWLAAENGMS LRLTTGEWMT TMPENLNMDW VDSVKHVFEY FTERTPRAYY ELRETSLVWN 720 YKYADVEFGR LQARDMLQHL WTGPISNAAV DVVQGGRSVE VRAVGITKGA AIDRILRQIV 780 HNQGMRTPID YVLCVGHFLA KDEDIYTFFE PEHPSESPGI VRTKSVPKVP YGGSRAQYHR 840 QKQRSLSTLE GNTAGSAGWR PIVSDRVSEH EGSSILDLKR ENYFSCAVSR KQSIARYLLG 900 TSDDVVALLK QMADSSSLT* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 55 | 919 | 888 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 89 | 913 | 825 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 94 | 560 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 93 | 560 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 94 | 849 | 766 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 86 | 915 | 82 | 919 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| RefSeq | NP_177979.1 | 0 | 85 | 917 | 81 | 940 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002305707.1 | 0 | 55 | 917 | 26 | 897 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002331300.1 | 0 | 90 | 917 | 83 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522296.1 | 0 | 32 | 919 | 4 | 915 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 119 | 570 | 20 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 119 | 570 | 20 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 119 | 570 | 20 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 119 | 570 | 20 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 119 | 570 | 20 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 383 | 455 | 836 | 0 |
| CV711307 | 293 | 92 | 384 | 0 |
| CX546763 | 292 | 92 | 383 | 0 |
| DW069910 | 288 | 180 | 467 | 0 |
| CB651133 | 279 | 122 | 400 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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