| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_021742m |
| Family | GH32 |
| Protein Properties | Length: 573 Molecular Weight: 64953.7 Isoelectric Point: 9.4951 |
| Chromosome | Chromosome/Scaffold: 02892 Start: 67903 End: 72504 |
| Description | cell wall invertase 2 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 48 | 361 | 0 |
| HFQPPMNWINGPMYYKGLYHLFYQYNPNGATWGNIVWAHSVSKDLVNWQALKHALYPSKWFDINGCWSGSVTILPGNKPVILYTGIDPNDREIQNYAVPK NLSDPYLRDWIKPDDNPMVDPDSNVNASAFRDPTTAWWVDGHWKIVVGSRRHHRGMAYLYRSRDFKTWIKAKHPLHSVPRTGNWECPDFFPVSLSGQNGL DTSVVGENVKHVFKVSLDITRYDYYTVGTYDRTRDKYIPDNTSVDGWAGLRFDYGNFYASKSFFDPSKSRRVLWGWVNESDTAADDNSKGWAGIQGVPRK IWLDSSKRQVVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 573 Download |
| MAVSKFLAVF GLLLVFCNNG VEGSHKIYAQ YQNVKAVKVN PVHRTGYHFQ PPMNWINGPM 60 YYKGLYHLFY QYNPNGATWG NIVWAHSVSK DLVNWQALKH ALYPSKWFDI NGCWSGSVTI 120 LPGNKPVILY TGIDPNDREI QNYAVPKNLS DPYLRDWIKP DDNPMVDPDS NVNASAFRDP 180 TTAWWVDGHW KIVVGSRRHH RGMAYLYRSR DFKTWIKAKH PLHSVPRTGN WECPDFFPVS 240 LSGQNGLDTS VVGENVKHVF KVSLDITRYD YYTVGTYDRT RDKYIPDNTS VDGWAGLRFD 300 YGNFYASKSF FDPSKSRRVL WGWVNESDTA ADDNSKGWAG IQGVPRKIWL DSSKRQVVQW 360 PVEELETLRR KKIELGIQKL KKGEKVEVKR ITATQADVEV TFSFPSLEKA EPFDHKWADI 420 DAQGVCAEKG STDQGGLGPF GLLTLASKEL EEFTPVFFRV FKAGNRHKVL LCSDARSSSL 480 REGLYKPSFA GFVDVDLSTK RLSLRSLIDH SVVSSFGEGG KTVITSRVYP TLAVFHKAHL 540 FVFNNGSETI TVEKFNAWSM KNPIMNRGFE TA* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-49 | 43 | 530 | 511 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 3.0e-61 | 21 | 531 | 532 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-90 | 53 | 364 | 325 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 8.0e-140 | 48 | 361 | 326 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-177 | 48 | 520 | 486 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABY81288.1 | 0 | 2 | 566 | 8 | 579 | cell-wall invertase [Populus alba x Populus grandidentata] |
| GenBank | ABY81289.1 | 0 | 17 | 566 | 21 | 575 | cell-wall invertase [Populus alba x Populus grandidentata] |
| RefSeq | XP_002309419.1 | 0 | 1 | 566 | 1 | 573 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002323420.1 | 0 | 7 | 566 | 11 | 576 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002530337.1 | 0 | 1 | 566 | 3 | 573 | Beta-fructofuranosidase, insoluble isoenzyme 3 precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 39 | 560 | 4 | 535 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 40 | 560 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 40 | 560 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 40 | 560 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 40 | 560 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
