| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_022890m |
| Family | CE10 |
| Protein Properties | Length: 327 Molecular Weight: 36758.8 Isoelectric Point: 6.5035 |
| Chromosome | Chromosome/Scaffold: 00341 Start: 1060606 End: 1061586 |
| Description | carboxyesterase 20 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 45 | 307 | 0 |
| NHVLSRDIPINHSNNTWVRVFLPDSSFSCSKLPLIVYFHGGGFINCSASSTIFHDFCSNMAADIPAIVVSFNYRLAPEHRLPAAYDDAVEVFQWIKNNSQ LDWMRECADYNRCFIMGSSAGANIAYHAGFRVSKEVDRLEPLKIKGLILHHPFIGGTQRTESELRLVNDLHLPQSVSDLMWELALPIGVDRDHEYCNPMA NGGSKLWDKMRLLGWRVLVTGCNGDPTIDRQMELVQMLRAKNVGITGHFSEGGYHVVELKEPS | |||
| Full Sequence |
|---|
| Protein Sequence Length: 327 Download |
| MSGQNSASNC RVNPYEYLQI LVNPDGTITR DPNRFPNSAP SLDSNHVLSR DIPINHSNNT 60 WVRVFLPDSS FSCSKLPLIV YFHGGGFINC SASSTIFHDF CSNMAADIPA IVVSFNYRLA 120 PEHRLPAAYD DAVEVFQWIK NNSQLDWMRE CADYNRCFIM GSSAGANIAY HAGFRVSKEV 180 DRLEPLKIKG LILHHPFIGG TQRTESELRL VNDLHLPQSV SDLMWELALP IGVDRDHEYC 240 NPMANGGSKL WDKMRLLGWR VLVTGCNGDP TIDRQMELVQ MLRAKNVGIT GHFSEGGYHV 300 VELKEPSKFK AMLLVLKEEN SWFVEQ* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 4.0e-9 | 64 | 142 | 88 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| pfam00135 | COesterase | 1.0e-9 | 29 | 166 | 158 | + Carboxylesterase family. | ||
| COG2272 | PnbA | 5.0e-10 | 75 | 169 | 106 | + Carboxylesterase type B [Lipid metabolism] | ||
| COG0657 | Aes | 3.0e-30 | 36 | 299 | 266 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 2.0e-54 | 79 | 300 | 222 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2O7R | 0 | 5 | 318 | 9 | 325 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| GenBank | ABB89013.1 | 0 | 5 | 318 | 9 | 325 | CXE carboxylesterase [Actinidia eriantha] |
| RefSeq | XP_002267605.1 | 0 | 1 | 317 | 1 | 326 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002322442.1 | 0 | 1 | 318 | 1 | 325 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002510874.1 | 0 | 1 | 317 | 1 | 323 | Gibberellin receptor GID1, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 0 | 5 | 318 | 9 | 325 | A Chain A, Polygalacturonase From Erwinia Carotovora Ssp. Carotovora |
| PDB | 2o7r_A | 0 | 5 | 318 | 9 | 325 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_F | 2e-38 | 20 | 300 | 32 | 315 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_E | 2e-38 | 20 | 300 | 32 | 315 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_D | 2e-38 | 20 | 300 | 32 | 315 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CO090564 | 262 | 13 | 270 | 0 |
| DY293901 | 301 | 12 | 298 | 0 |
| FC882608 | 283 | 12 | 280 | 0 |
| FC918394 | 284 | 12 | 281 | 0 |
| DY306635 | 298 | 12 | 295 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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