| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_023440m |
| Family | GT20 |
| Protein Properties | Length: 914 Molecular Weight: 103620 Isoelectric Point: 6.9019 |
| Chromosome | Chromosome/Scaffold: 06582 Start: 337073 End: 347293 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 80 | 548 | 0 |
| HKQRLLVVANRLPVSAVRKGEDSWQLEMSVGGLVSALLGVKEFDARWIGWAGVNVPDEIGQKALTTALAEKRCIPVFLDEDIVHQYYNGYCNNMLWPLFH YLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKHYKEGDVVWCHDYHLMFLPKCLKEYNSKMKVGWFLHTPFPSSEIHRMLPSRSELLRSVLAADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRVAAFPIGIDSERFIRALELPQVQDHIKELKERFAGRKVMLGVDRLDMIKGIPQKILAFEEFLEE NPEWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLI LSEFAGAAQSLGAGAILVNPWNISEVASSIGYALNMPADEREKRHNHNFRHVTTHTSQEWAATFVSELN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 914 Download |
| MPGNKYNGTP VTPRTRLERL LLNRELRKFN RFFNPSDELD DGLKEAELFA NDSVLNKDEN 60 CGPFNEETFS EGVDGSERLH KQRLLVVANR LPVSAVRKGE DSWQLEMSVG GLVSALLGVK 120 EFDARWIGWA GVNVPDEIGQ KALTTALAEK RCIPVFLDED IVHQYYNGYC NNMLWPLFHY 180 LGLPQEDRLA TTRSFQSQFD AYKKANQMFA DVVNKHYKEG DVVWCHDYHL MFLPKCLKEY 240 NSKMKVGWFL HTPFPSSEIH RMLPSRSELL RSVLAADLVG FHTYDYARHF VSACTRILGL 300 EGTPEGVEDQ GKLTRVAAFP IGIDSERFIR ALELPQVQDH IKELKERFAG RKVMLGVDRL 360 DMIKGIPQKI LAFEEFLEEN PEWRDKVVLL QIAVPTRTDV PEYQKLTSQV HEIVGRINGR 420 FGTLTAVPIH HLDRSLDFHA LCALYAVTDV ALVTSLRDGM NLVSYEFVAC QASKKGVLIL 480 SEFAGAAQSL GAGAILVNPW NISEVASSIG YALNMPADER EKRHNHNFRH VTTHTSQEWA 540 ATFVSELNDT IVEAQLRTRQ VLPLLPVNDA VERYTQSSNR LLILGFNATL TEPVHTLGRR 600 GSQIREMEPR LHPQLKEPLK KLCKDQMTTV VVLSGSDRTI LDENFGEYNM WLAAENGMFL 660 RRTTGKWMTT MPENLNMDWV DSVKHVFEYF TERTPRSHFE LRETSLLWNY KYADVEFGRL 720 QARDMLQHLW TGPISNAAVD VVQGRRSVEV RAVGVTKGAA IDRILGEIVH NQGMKAPIDY 780 VLCVGHFLVK DEDIYTFFEP ELPIETPPIV RCRLPEPVGL PVPKIPCGRS RSKTHLKKQR 840 SLSTLEGNYL GSGGWRPMVD DRISVHEGSS VLDLKGENYF SCAVSRKRSN ARYLLGTTDD 900 VVTLLKQLAD CLH* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 910 | 928 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 78 | 909 | 832 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 83 | 549 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 82 | 549 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 83 | 765 | 692 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 1 | 911 | 1 | 919 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| RefSeq | NP_177979.1 | 0 | 1 | 910 | 1 | 937 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002305707.1 | 0 | 24 | 910 | 4 | 894 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002331300.1 | 0 | 1 | 911 | 1 | 920 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522296.1 | 0 | 1 | 910 | 1 | 910 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 83 | 559 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 83 | 559 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 83 | 559 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 83 | 559 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 83 | 559 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CX546763 | 315 | 58 | 372 | 0 |
| CV711307 | 293 | 81 | 373 | 0 |
| ES813375 | 391 | 444 | 824 | 0 |
| DW069910 | 288 | 169 | 456 | 0 |
| CB651133 | 280 | 110 | 389 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
