| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_024481m |
| Family | GT35 |
| Protein Properties | Length: 739 Molecular Weight: 83945.4 Isoelectric Point: 7.0796 |
| Chromosome | Chromosome/Scaffold: 04851 Start: 444852 End: 453089 |
| Description | alpha-glucan phosphorylase 2 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 295 | 738 | 0 |
| ALSQLGFDFEVLAEQEGDAALGNGGLARLSACQMDSLATLDYPAWGYGLRYQYGLFRQVILDGYQHEQPDYWLNFGNPWEIERIHVTYTVKFYGTVEEEN FKGGKHKVWVPNETVEAVAYDNLIPGYGTRNTITLRLWAAKPSDQNDMESYNTGDYINAVVNRQRAETISSILYPDDRSHQGKELRLKQQYFFVSASLQD IIRRFKDTHSNFDDFPEKVALQLNDTHPSLAIAEVMRVLVDEENLSWSRAWDISCKIFTFTTHTVLPEASEKIPVDLLGSLLPRHLQIIYEINFRFLEEL KKRIGLDYDRLSRMSIIEEGAVKSIRMANLSIVCSHTVNGVSKMHAELLKTRVFKDFYELWPHKFHYKTNGVTQRRWVVVSNPSLCALISKWLGTEAWIR DMDLLVGLQEYATNGDLHEEWKMVRKVNKMRLAEYIEAMSGVKV | |||
| Full Sequence |
|---|
| Protein Sequence Length: 739 Download |
| MSPITLPLSQ SKLLFPSFTG VSLSSVSASS NKHSSISIPY NASHFNFPRL ASTSGSLRAP 60 SAASTTTITT SVVDGTEDDH SDLGGGATAF VIHSRKRIGL LQVIIRVFKV LGLIVEKATV 120 EYEGDYFTKT FYVTDSSGNR IEDAESLDKI KKVLMQAIES GERGAAEVKT GSTGRGIVVR 180 RPGLRLGENR AKVERMFGLM DRFLKNDPAT LQNDIVNHVE YTVARSRFSF DDFEAYQALT 240 HSVRDRLIER WHDTQICFRR KDPKRLYFLS LEYLMGRSLS NSVINLGIRD QYADALSQLG 300 FDFEVLAEQE GDAALGNGGL ARLSACQMDS LATLDYPAWG YGLRYQYGLF RQVILDGYQH 360 EQPDYWLNFG NPWEIERIHV TYTVKFYGTV EEENFKGGKH KVWVPNETVE AVAYDNLIPG 420 YGTRNTITLR LWAAKPSDQN DMESYNTGDY INAVVNRQRA ETISSILYPD DRSHQGKELR 480 LKQQYFFVSA SLQDIIRRFK DTHSNFDDFP EKVALQLNDT HPSLAIAEVM RVLVDEENLS 540 WSRAWDISCK IFTFTTHTVL PEASEKIPVD LLGSLLPRHL QIIYEINFRF LEELKKRIGL 600 DYDRLSRMSI IEEGAVKSIR MANLSIVCSH TVNGVSKMHA ELLKTRVFKD FYELWPHKFH 660 YKTNGVTQRR WVVVSNPSLC ALISKWLGTE AWIRDMDLLV GLQEYATNGD LHEEWKMVRK 720 VNKMRLAEYI EAMSGVKV* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0058 | GlgP | 7.0e-166 | 203 | 738 | 537 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| PRK14986 | PRK14986 | 4.0e-175 | 211 | 730 | 520 | + glycogen phosphorylase; Provisional | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 212 | 738 | 527 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 215 | 738 | 524 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| pfam00343 | Phosphorylase | 0 | 295 | 738 | 444 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI30609.1 | 0 | 200 | 738 | 1 | 539 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_001757919.1 | 0 | 200 | 738 | 1 | 539 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_002273615.1 | 0 | 196 | 738 | 1 | 543 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002305114.1 | 0 | 196 | 738 | 1 | 543 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002509431.1 | 0 | 1 | 738 | 1 | 674 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1ygp_B | 0 | 206 | 731 | 39 | 583 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1ygp_A | 0 | 206 | 731 | 39 | 583 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 4el5_A | 0 | 211 | 731 | 16 | 539 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 4el0_A | 0 | 211 | 731 | 16 | 539 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 4eky_A | 0 | 211 | 731 | 16 | 539 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO779924 | 618 | 108 | 720 | 0 |
| CO095011 | 285 | 427 | 711 | 0 |
| GW477823 | 247 | 313 | 559 | 0 |
| ES340735 | 345 | 288 | 631 | 0 |
| ES328847 | 344 | 300 | 642 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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