| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_026462m |
| Family | PL4 |
| Protein Properties | Length: 633 Molecular Weight: 72154.9 Isoelectric Point: 5.3979 |
| Chromosome | Chromosome/Scaffold: 02895 Start: 532400 End: 538142 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 1 | 608 | 0 |
| VVVDNGFMQLTISNPEGIVTGIQYNGISNLLEVLNDENDRGYWDLVWSDETTTRKKGNFDRMEGTSFKVIQQNEKQIELSFTRTWNSSLHGKVVPLNIDK RFVVLGGTSGFYTYAIYEHLKEWPAFDLDNTRIVFKLRNDKFHYMAIADDRQRYMPLPEDRREGRGQVLAYPEAVLLVNPVEPQFKGEVDDKYQYSCESR NNGVHGWICDDPAVGFWLITPSYEFRSGGPLKQFLTSHVGPTTLSVLHSTHYLGADMIAKFVANEPWKKVYGPVFVYLNSLLDHGDPLSLWQDAQKQMMN EVQRWPYDFLASQDFPPSDQRGKVSGRLLVKDRYVSDGNISADGAYVGLASPGDAGSWQLEAKGYQFWTETDKDGQFTINNIRPGVYNLYAWVPGFIGDY KYETLINISAGGNITVDDIVYEPPRNGSTLWEIGIPDRVASEFYIPDPNPKYVNKLYLNHTDRYRQYGLWERYAELYPDGDLVFTIGTSNYTKDWFFQQV TRKINNSTYEGTTWQIKFKLDEIDQNWTYTIRLALATANNANLQVKINDLNGNSSLLSTGVFGKDNTIARHGIHGLYRLFNISIPGAQLLQGNNTIFLTQ ASGTSPFE | |||
| Full Sequence |
|---|
| Protein Sequence Length: 633 Download |
| VVVDNGFMQL TISNPEGIVT GIQYNGISNL LEVLNDENDR GYWDLVWSDE TTTRKKGNFD 60 RMEGTSFKVI QQNEKQIELS FTRTWNSSLH GKVVPLNIDK RFVVLGGTSG FYTYAIYEHL 120 KEWPAFDLDN TRIVFKLRND KFHYMAIADD RQRYMPLPED RREGRGQVLA YPEAVLLVNP 180 VEPQFKGEVD DKYQYSCESR NNGVHGWICD DPAVGFWLIT PSYEFRSGGP LKQFLTSHVG 240 PTTLSVLHST HYLGADMIAK FVANEPWKKV YGPVFVYLNS LLDHGDPLSL WQDAQKQMMN 300 EVQRWPYDFL ASQDFPPSDQ RGKVSGRLLV KDRYVSDGNI SADGAYVGLA SPGDAGSWQL 360 EAKGYQFWTE TDKDGQFTIN NIRPGVYNLY AWVPGFIGDY KYETLINISA GGNITVDDIV 420 YEPPRNGSTL WEIGIPDRVA SEFYIPDPNP KYVNKLYLNH TDRYRQYGLW ERYAELYPDG 480 DLVFTIGTSN YTKDWFFQQV TRKINNSTYE GTTWQIKFKL DEIDQNWTYT IRLALATANN 540 ANLQVKINDL NGNSSLLSTG VFGKDNTIAR HGIHGLYRLF NISIPGAQLL QGNNTIFLTQ 600 ASGTSPFEGI MYDYIRFEGP PSSNTIKNTR FR* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam13620 | CarboxypepD_reg | 0.0001 | 369 | 417 | 49 | + Carboxypeptidase regulatory-like domain. | ||
| cd10316 | RGL4_M | 1.0e-32 | 320 | 419 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 2.0e-46 | 431 | 618 | 190 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 3.0e-69 | 1 | 280 | 283 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 6.0e-93 | 1 | 189 | 189 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_002285626.1 | 0 | 3 | 625 | 1 | 620 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
| RefSeq | XP_002301113.1 | 0 | 1 | 621 | 59 | 677 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527353.1 | 0 | 1 | 623 | 15 | 636 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527356.1 | 0 | 1 | 627 | 128 | 753 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527356.1 | 0 | 62 | 189 | 1 | 128 | lyase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1nkg_A | 0.00000005 | 341 | 605 | 255 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 3njv_A | 0.00000005 | 341 | 605 | 255 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 2xhn_B | 0.00000005 | 341 | 605 | 255 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 2xhn_A | 0.00000005 | 341 | 605 | 255 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 3njx_A | 0.00000005 | 341 | 605 | 255 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DT552229 | 293 | 3 | 294 | 0 |
| DW479599 | 281 | 1 | 281 | 0 |
| DW479600 | 282 | 4 | 285 | 0 |
| DY293973 | 338 | 1 | 335 | 0 |
| GE636266 | 244 | 292 | 532 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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