| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_026805m |
| Family | GT8 |
| Protein Properties | Length: 630 Molecular Weight: 73254 Isoelectric Point: 7.5591 |
| Chromosome | Chromosome/Scaffold: 06948 Start: 399011 End: 401655 |
| Description | plant glycogenin-like starch initiation protein 1 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 299 | 509 | 4.80001e-41 |
| YATILHSAHVYVCGAIAAAQSIRLSGSNRDLVILVDESISVYHRSGLEAAGWKIRTIKRIRNPKAEKDAYNEWNYSKFRLWQLTDYDKIIFIDADLLILR NIDFLFGMPEISATGNNATLFNSGVMVIEPSNCTFQLLMEHINEIESYNGGDQGYLNEIFTWWHRIPKHMNFLKHFWVGDEEEVKQKKIRLFGSEPPVLY VLHYLGVKPWL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 630 Download |
| MGNSPANVEA RHRLSSSIED IYKRRLQKNK VKGIEKPLHI PIQDRSSCCK FPLLKLAFLI 60 LICCTIVTLF RSPEVDLSHS SSRWIWGGSD PRYFSDIDMN WDSLMKVIEK LGEQNDYQGI 120 GLLNFNKTEI NHWKQLLPDV THVDLQLDYA DRNLTWESLY PEWIDEEQEK EVPVCPSLPK 180 VDVPRKRLDL IAVKLPCRNE WNWSRDVARL HLQIAAASLA ASAKGSYPVH LLFITKRFPV 240 PNLFPCKELV VREGNVWLYK PDLNVLREKL QLPVGSCELA LPLRDKEPFY SGNPRREAYA 300 TILHSAHVYV CGAIAAAQSI RLSGSNRDLV ILVDESISVY HRSGLEAAGW KIRTIKRIRN 360 PKAEKDAYNE WNYSKFRLWQ LTDYDKIIFI DADLLILRNI DFLFGMPEIS ATGNNATLFN 420 SGVMVIEPSN CTFQLLMEHI NEIESYNGGD QGYLNEIFTW WHRIPKHMNF LKHFWVGDEE 480 EVKQKKIRLF GSEPPVLYVL HYLGVKPWLC FRDYDCNWNV DIFQEFASDI AHARWWKVHD 540 AMPEKLHQFC SLKSKQKAQL EWDRRQAEQT NYTDGHWKIK IQDHRLKKCI DNLCNWKSML 600 RHWGESNWTD DEFFNPTPPA ISTSSLSGL* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00505 | Glyco_transf_8 | 1.0e-9 | 302 | 508 | 248 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| cd06914 | GT8_GNT1 | 2.0e-15 | 309 | 459 | 161 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| PLN00176 | PLN00176 | 2.0e-15 | 278 | 508 | 274 | + galactinol synthase | ||
| pfam01501 | Glyco_transf_8 | 1.0e-39 | 300 | 510 | 255 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 6.0e-71 | 297 | 542 | 256 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAX33317.1 | 0 | 1 | 629 | 1 | 636 | secondary cell wall-related glycosyltransferase family 8 [Populus tremula x Populus tremuloides] |
| RefSeq | XP_002275240.1 | 0 | 1 | 628 | 1 | 636 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002307022.1 | 0 | 96 | 629 | 11 | 544 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002310513.1 | 0 | 1 | 629 | 1 | 630 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002517237.1 | 0 | 13 | 629 | 16 | 630 | glycogenin, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2t_A | 2e-20 | 287 | 508 | 16 | 242 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2x_B | 3e-20 | 297 | 508 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2x_A | 3e-20 | 297 | 508 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2v_B | 3e-20 | 297 | 508 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2v_A | 3e-20 | 297 | 508 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FG552677 | 321 | 198 | 517 | 0 |
| DY825283 | 301 | 315 | 615 | 0 |
| CO253323 | 301 | 255 | 555 | 0 |
| EX355703 | 296 | 257 | 552 | 0 |
| GR707193 | 283 | 273 | 555 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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