| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_027037m |
| Family | AA7 |
| Protein Properties | Length: 528 Molecular Weight: 59716 Isoelectric Point: 9.7694 |
| Chromosome | Chromosome/Scaffold: 05656 Start: 32836 End: 34419 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 73 | 524 | 0 |
| TSDTPKPLAIVVALHESHVQATVICAKFHSLQIRIRSGGHDFEGLSYRSDVPFVILDLFNLRSIDIDIDSETAWVQSGAILGELYYAIAKKSKVHGFPAG VCPSIGVGGHFSGGGYGNMMRKYGLSVDHVIDAKLVDVNGKILNRKSMGEDLFWAIRGGGGASFGVILSWKIKLVRVPEKVTVFMVDRTLEEGATDIVYQ WQAINKLDKELFIRAQPEVRRNDKDEKTVRVRFIALFLGNTKKLLSLLKKSIPKLGLKQEDCKEVSWLESTMFWTNIPLGTPTEALLNRSIPAELFFKSK SDYVKKIISKHDLENIWKQFMKTEGMVLQWNPYGGRMKEISASETPFPHRAGYLFKIQYFTLWFTQGTEASDRHIRLAREMYDSMAPHVSKNPREAYLNY RDLDIGRNPSNETNFQEAQVYGSKYFKGNFLKLVAVKKRVDPDNFFKNEQSI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 528 Download |
| MKVSISITQI VLSVLLLLFS APTSYPQVLE NFLNCLPNHV HGSNPIFEAI YTPSNSSFQS 60 VLVAYIKNRR FLTSDTPKPL AIVVALHESH VQATVICAKF HSLQIRIRSG GHDFEGLSYR 120 SDVPFVILDL FNLRSIDIDI DSETAWVQSG AILGELYYAI AKKSKVHGFP AGVCPSIGVG 180 GHFSGGGYGN MMRKYGLSVD HVIDAKLVDV NGKILNRKSM GEDLFWAIRG GGGASFGVIL 240 SWKIKLVRVP EKVTVFMVDR TLEEGATDIV YQWQAINKLD KELFIRAQPE VRRNDKDEKT 300 VRVRFIALFL GNTKKLLSLL KKSIPKLGLK QEDCKEVSWL ESTMFWTNIP LGTPTEALLN 360 RSIPAELFFK SKSDYVKKII SKHDLENIWK QFMKTEGMVL QWNPYGGRMK EISASETPFP 420 HRAGYLFKIQ YFTLWFTQGT EASDRHIRLA REMYDSMAPH VSKNPREAYL NYRDLDIGRN 480 PSNETNFQEA QVYGSKYFKG NFLKLVAVKK RVDPDNFFKN EQSIPPL* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR00387 | glcD | 1.0e-5 | 84 | 271 | 204 | + glycolate oxidase, subunit GlcD. This protein, the glycolate oxidase GlcD subunit, is similar in sequence to that of several D-lactate dehydrogenases, including that of E. coli. The glycolate oxidase has been found to have some D-lactate dehydrogenase activity [Energy metabolism, Other]. | ||
| pfam08031 | BBE | 8.0e-16 | 468 | 525 | 58 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| COG0277 | GlcD | 2.0e-17 | 59 | 271 | 226 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 1.0e-22 | 79 | 216 | 139 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_002299007.1 | 0 | 30 | 526 | 25 | 523 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002299010.1 | 0 | 14 | 525 | 3 | 515 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002300550.1 | 0 | 32 | 525 | 1 | 494 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002317074.1 | 0 | 10 | 526 | 1 | 514 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002529891.1 | 0 | 27 | 527 | 16 | 519 | Reticuline oxidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3vte_A | 0 | 30 | 527 | 5 | 513 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 4dns_B | 0 | 21 | 527 | 1 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 21 | 527 | 1 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_B | 0 | 26 | 527 | 5 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_A | 0 | 26 | 527 | 5 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
