| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_027331m |
| Family | GH31 |
| Protein Properties | Length: 440 Molecular Weight: 49885.5 Isoelectric Point: 5.9841 |
| Chromosome | Chromosome/Scaffold: 05775 Start: 85892 End: 98727 |
| Description | heteroglycan glucosidase 1 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 1 | 394 | 0 |
| MPPKWSLGYQQCRWSYDSDTRVREVARTFRKKGIPCDVIWMDIDYMDGFRCFTFDQERFPDPQSLVKDLHDIGFNAVWMLDPGIKCEEGYFVYDSGSKID VWIQTAEGRPFIGEVWPGPCVFPDFTQSKVRSWWASLVKAFSSNGVDGIWNDMNEPAVFKTVTKTMSERNIHRGDDELGGYQEHSYYHNVYGMLMARSTF EGMKLANENKRPFVLTRAGFIGSQKYAATWTGDNLSNWEHLHMSIPMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMGIGAMFPFCRGHSETGTFDHE PWSFGEECEEVCRLALKRRYRLIPHLYTLFYAAHTTGTPVATPTFFADLKNPSLRTLENSFLLGSLLVYASTIADQGTNKLQHDLPKGIWLRFD | |||
| Full Sequence |
|---|
| Protein Sequence Length: 440 Download |
| MPPKWSLGYQ QCRWSYDSDT RVREVARTFR KKGIPCDVIW MDIDYMDGFR CFTFDQERFP 60 DPQSLVKDLH DIGFNAVWML DPGIKCEEGY FVYDSGSKID VWIQTAEGRP FIGEVWPGPC 120 VFPDFTQSKV RSWWASLVKA FSSNGVDGIW NDMNEPAVFK TVTKTMSERN IHRGDDELGG 180 YQEHSYYHNV YGMLMARSTF EGMKLANENK RPFVLTRAGF IGSQKYAATW TGDNLSNWEH 240 LHMSIPMVLQ LGLSGQPLSG PDIGGFAGNA TPKLFGRWMG IGAMFPFCRG HSETGTFDHE 300 PWSFGEECEE VCRLALKRRY RLIPHLYTLF YAAHTTGTPV ATPTFFADLK NPSLRTLENS 360 FLLGSLLVYA STIADQGTNK LQHDLPKGIW LRFDFHDSHP DLPTLYLQGG SIIPLGPPHQ 420 HVGEANLSDD LTLVVALDEY 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06602 | GH31_MGAM_SI_GAA | 6.0e-121 | 1 | 346 | 352 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
| cd06603 | GH31_GANC_GANAB_alpha | 5.0e-131 | 1 | 337 | 343 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
| pfam01055 | Glyco_hydro_31 | 5.0e-159 | 1 | 413 | 431 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| PLN02763 | PLN02763 | 0 | 1 | 440 | 440 | + hydrolase, hydrolyzing O-glycosyl compounds | ||
| cd06604 | GH31_glucosidase_II_MalA | 0 | 1 | 337 | 338 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI37476.1 | 0 | 1 | 440 | 260 | 699 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_566736.1 | 0 | 1 | 439 | 194 | 632 | HGL1 (heteroglycan glucosidase 1); hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| RefSeq | XP_002263148.1 | 0 | 1 | 440 | 177 | 615 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002326592.1 | 0 | 1 | 439 | 204 | 639 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002519886.1 | 0 | 1 | 440 | 194 | 633 | neutral alpha-glucosidase ab precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3w38_A | 0 | 3 | 422 | 318 | 750 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 3w37_A | 0 | 3 | 422 | 318 | 750 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 2g3n_F | 0 | 1 | 415 | 171 | 610 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 2g3n_E | 0 | 1 | 415 | 171 | 610 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 2g3n_D | 0 | 1 | 415 | 171 | 610 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO783906 | 313 | 34 | 346 | 0 |
| CV196735 | 274 | 145 | 418 | 0 |
| DT727274 | 299 | 84 | 382 | 0 |
| EG431953 | 280 | 131 | 410 | 0 |
| HO783906 | 76 | 280 | 355 | 0.003 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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