| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_029065m |
| Family | GT20 |
| Protein Properties | Length: 862 Molecular Weight: 97701.9 Isoelectric Point: 6.5387 |
| Chromosome | Chromosome/Scaffold: 12794 Start: 1039241 End: 1042001 |
| Description | trehalose-phosphatase/synthase 9 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 53 | 546 | 0 |
| PSARCYKKKIIVANFLPLHAQKDLETSKWSFSFDEDSLLFQMKDGFSDNTEIVYVGSLKVDVDASEQEEVSQKLLEEYNCVPTFLPPELHKKFYHGFCKH QLWPLFHYMLPMCPDHSNRFDKLLWQAYVSANKIFADKVMEVINPEEDHVWVHDYHLMVLPTFLRKHFYRVKLGFFLHSPFPSSEIYRTLPVRDEILKAL LNADLIGFHTFDYARHFLSCCSRMLGLDYESKRGHIGLEYFGRIVYIKILPAGIHLGRLESALNHPSSSIKVKEIQTQFEGKKIIVGVDDMDIFKGISLK LLAMEQLLQQNPELRGKVVMVQILNPARSSGKDVQEAERETYLTTKRINSIFGFPGYDPVILIDRHVPLYEKTAYYSLAECCIVNAVRDGMNLIPYEYIV CRQGTPKMDEALGVDPRSSHASTIVVSEFIGCSPSLSGAIRVNPWDVEAVADALNLALTMPDLEKQLRHEKHYRYISSHDVAYWVRSFVLDLER | |||
| Full Sequence |
|---|
| Protein Sequence Length: 862 Download |
| MMSKSCINFL DLSSGDMLNF SRTPRSLPRV MTVEGIFSNT DSDGSNDGDT NVPSARCYKK 60 KIIVANFLPL HAQKDLETSK WSFSFDEDSL LFQMKDGFSD NTEIVYVGSL KVDVDASEQE 120 EVSQKLLEEY NCVPTFLPPE LHKKFYHGFC KHQLWPLFHY MLPMCPDHSN RFDKLLWQAY 180 VSANKIFADK VMEVINPEED HVWVHDYHLM VLPTFLRKHF YRVKLGFFLH SPFPSSEIYR 240 TLPVRDEILK ALLNADLIGF HTFDYARHFL SCCSRMLGLD YESKRGHIGL EYFGRIVYIK 300 ILPAGIHLGR LESALNHPSS SIKVKEIQTQ FEGKKIIVGV DDMDIFKGIS LKLLAMEQLL 360 QQNPELRGKV VMVQILNPAR SSGKDVQEAE RETYLTTKRI NSIFGFPGYD PVILIDRHVP 420 LYEKTAYYSL AECCIVNAVR DGMNLIPYEY IVCRQGTPKM DEALGVDPRS SHASTIVVSE 480 FIGCSPSLSG AIRVNPWDVE AVADALNLAL TMPDLEKQLR HEKHYRYISS HDVAYWVRSF 540 VLDLERACKD HYSKRCWGIG FGLNFRILSL SPNFRKLSSE HIVSAYKRTY RRVIFLDYDG 600 TVVPQTSIVK TPSPEVISVL SNLCTDPKNT VFIVSGRAKD TLSDWFTQCE NLGIAAEHGY 660 FMRWSRMSNW ETSNLVADFD WKKIALPVMK SYTEATDGSY IETKESALVW NHQYADPVFG 720 SCQAKELLDH LETVLANEPV VVKRGQHIVE VKPQGVTKGL VAEKVLYAMV TSGKSPDFVM 780 CIGDDRSDED MFESISRKGS SWSFNSAPEI FACTVGQKPS KARYYLDDTV DVLALLQGLA 840 AASSSKPRGS EEVQVSFDNV I* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 9.0e-175 | 62 | 832 | 812 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 832 | 837 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 59 | 546 | 492 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 59 | 832 | 783 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 0 | 62 | 545 | 484 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN78769.1 | 0 | 1 | 861 | 1 | 857 | hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002283215.1 | 0 | 1 | 860 | 1 | 861 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002318643.1 | 0 | 1 | 861 | 1 | 857 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002322159.1 | 0 | 1 | 861 | 1 | 861 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002511428.1 | 0 | 1 | 859 | 1 | 859 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 144 | 546 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 144 | 546 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 144 | 546 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 144 | 546 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 144 | 546 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 760 | 79 | 832 | 0 |
| HO779327 | 515 | 99 | 613 | 0 |
| HO779327 | 253 | 610 | 862 | 0 |
| HO797286 | 456 | 377 | 832 | 0 |
| HO777775 | 78 | 1 | 77 | 0.052 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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