| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_029100m |
| Family | AA7 |
| Protein Properties | Length: 530 Molecular Weight: 59971.5 Isoelectric Point: 9.151 |
| Chromosome | Chromosome/Scaffold: 10493 Start: 1053854 End: 1056265 |
| Description | cytokinin oxidase 3 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 63 | 264 | 3.3e-28 |
| GNIVHENPAAVLHPSSPQDIVSLIKFAYNNSGTFTISARGRSHSVFGQAMAPNGVIVDMMRLRSHRENNGVIVSKNPSLGYFYADVGGEQLWIDVLKATM EHGLAPVAWTDYLYLTVGGTLSNAGISGQSFRYGPQISNVYEMDVVTGKGELVTCSPHKNSELFYAVLGGLGQFGIITRARIAIEPAPKRVKWVRMLYSD FS | |||
| Full Sequence |
|---|
| Protein Sequence Length: 530 Download |
| MAVNYSVPAY FILILLFISR IRSTVGKSKP STNFPPPNLL TLDFSERIRT DPLAIESASS 60 DYGNIVHENP AAVLHPSSPQ DIVSLIKFAY NNSGTFTISA RGRSHSVFGQ AMAPNGVIVD 120 MMRLRSHREN NGVIVSKNPS LGYFYADVGG EQLWIDVLKA TMEHGLAPVA WTDYLYLTVG 180 GTLSNAGISG QSFRYGPQIS NVYEMDVVTG KGELVTCSPH KNSELFYAVL GGLGQFGIIT 240 RARIAIEPAP KRVKWVRMLY SDFSAFTKDQ ERLIAINGRR QSSALDYVEG SLLMNQGPPN 300 NWRSSFFPSS DIPRIMSLVT QHGIIYCLEV AKYYDDSTQH SVPKELEQMY KGLSFISGFK 360 LEKDVSFMDF LDRVRNGELK LQSQGLWDVP HPWLNLFLPK SRISDFNSGV FQDIVLKRNI 420 TTGPVLIYPM NRNKWDDRTS AVIPEEEVFY TVGFLHSSGL NDWQRYDDQN KEVLKFCEKG 480 GIKVKEYFPH NKTKEEWINH FGSKWKIFRE RKSMFDPKMM LSPGQRIFN* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01677 | pln_FAD_oxido | 3.0e-6 | 71 | 269 | 209 | + plant-specific FAD-dependent oxidoreductase. This model represents an uncharacterized plant-specific family of FAD-dependent oxidoreductases. At least seven distinct members are found in Arabidopsis thaliana. The family shows considerable sequence similarity to three different enzymes of ascorbic acid biosynthesis: L-galactono-1,4-lactone dehydrogenase (EC 1.3.2.3) from higher plants, D-arabinono-1,4-lactone oxidase (EC 1.1.3.37 from Saccharomyces cerevisiae, and L-gulonolactone oxidase (EC 1.1.3.8) from mouse, as well as to a bacterial sorbitol oxidase. The class of compound acted on by members of this family is unknown. | ||
| COG0277 | GlcD | 9.0e-28 | 45 | 525 | 495 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 4.0e-28 | 70 | 218 | 150 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 5.0e-144 | 250 | 528 | 284 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 29 | 529 | 504 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABN05760.1 | 0 | 1 | 529 | 1 | 538 | FAD linked oxidase, N-terminal [Medicago truncatula] |
| GenBank | ACM79256.1 | 0 | 1 | 529 | 1 | 524 | cytokinin oxidase/dehydrogenase [Gossypium hirsutum] |
| RefSeq | XP_002279519.1 | 0 | 1 | 529 | 1 | 519 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002308300.1 | 0 | 12 | 529 | 11 | 527 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002514119.1 | 0 | 1 | 529 | 1 | 529 | gulonolactone oxidase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3s1d_A | 0 | 36 | 529 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 36 | 529 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 36 | 529 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3c0p_A | 0 | 36 | 529 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3bw7_A | 0 | 36 | 529 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
