| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_029675m |
| Family | PL4 |
| Protein Properties | Length: 617 Molecular Weight: 70977.4 Isoelectric Point: 5.3201 |
| Chromosome | Chromosome/Scaffold: 01828 Start: 135107 End: 138316 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 1 | 599 | 0 |
| VVIDNGIIQVTFSSPDGEVIGIKYKEIDNLLEIANKEQNRGYWDVVWNRPGEHIIIDKLQATDFEIIMENEDQVEVSFSKKWRPSMDKFTVPLNIDKRYI VRRGSSGLYLYTILERLEGWPDVDMDQIRVVFKLQNAKFHFMAVSDDRQRIMPMPLDRITGQPLAYPEAVLLTNPINPKLRGEVDDKYQYSCEDKDNKVH GWISNDPPVGFWMITPSNEFRAGGPIKQDLTSHVGPVVLNMFTSTHYAGKDLNTKYRNGEPWKKVLGPAYVYLNSISPSEDPQALWQDAKEQMSIEVKRW PYNFPQSEDFPSSDQRGSVLGELRVRDRYISERLIYASYAYVGLAAPGDVGSWQMEAKGYQFWTQANKKGIFSIENVRAGKYSLYAWVPGIIGDYKYSVD IIIQPGSKIELGVLVYDPPRNGPTLWEIGIPDRTATEFYVPEAYPTLMNKLYTDLPTDKFRQYGLWEKYADLYPKNDVIYNVGVSNYNQDWFFAHVTREI GNRTYEATTWQIIFELDNVNPIGDYTLQVALASATHSELQVRVNAHRRLFTTGLIGKDNAIARHGIHGLYWLFSIQIPYFQLLEGNNTIYLTQKRSESP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 617 Download |
| VVIDNGIIQV TFSSPDGEVI GIKYKEIDNL LEIANKEQNR GYWDVVWNRP GEHIIIDKLQ 60 ATDFEIIMEN EDQVEVSFSK KWRPSMDKFT VPLNIDKRYI VRRGSSGLYL YTILERLEGW 120 PDVDMDQIRV VFKLQNAKFH FMAVSDDRQR IMPMPLDRIT GQPLAYPEAV LLTNPINPKL 180 RGEVDDKYQY SCEDKDNKVH GWISNDPPVG FWMITPSNEF RAGGPIKQDL TSHVGPVVLN 240 MFTSTHYAGK DLNTKYRNGE PWKKVLGPAY VYLNSISPSE DPQALWQDAK EQMSIEVKRW 300 PYNFPQSEDF PSSDQRGSVL GELRVRDRYI SERLIYASYA YVGLAAPGDV GSWQMEAKGY 360 QFWTQANKKG IFSIENVRAG KYSLYAWVPG IIGDYKYSVD IIIQPGSKIE LGVLVYDPPR 420 NGPTLWEIGI PDRTATEFYV PEAYPTLMNK LYTDLPTDKF RQYGLWEKYA DLYPKNDVIY 480 NVGVSNYNQD WFFAHVTREI GNRTYEATTW QIIFELDNVN PIGDYTLQVA LASATHSELQ 540 VRVNAHRRLF TTGLIGKDNA IARHGIHGLY WLFSIQIPYF QLLEGNNTIY LTQKRSESPF 600 QGIMYDYIRL EVPAET* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd10316 | RGL4_M | 8.0e-30 | 315 | 414 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 1.0e-49 | 426 | 611 | 188 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 9.0e-77 | 1 | 275 | 283 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 2.0e-86 | 1 | 184 | 186 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_179847.1 | 0 | 1 | 616 | 62 | 677 | lyase [Arabidopsis thaliana] |
| RefSeq | XP_002308510.1 | 0 | 1 | 616 | 57 | 674 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002319997.1 | 0 | 1 | 616 | 1 | 620 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527229.1 | 0 | 1 | 615 | 54 | 669 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527245.1 | 0 | 1 | 616 | 2 | 621 | lyase, putative [Ricinus communis] |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DY969340 | 323 | 212 | 534 | 0 |
| EH792178 | 287 | 289 | 570 | 0 |
| EE260349 | 253 | 368 | 617 | 0 |
| GW864372 | 311 | 130 | 438 | 0 |
| JG640880 | 272 | 138 | 407 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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