| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_029993m |
| Family | PL4 |
| Protein Properties | Length: 675 Molecular Weight: 76524.6 Isoelectric Point: 9.9676 |
| Chromosome | Chromosome/Scaffold: 02831 Start: 49699 End: 53143 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 48 | 654 | 0 |
| VQLQVTDKQVVVDNGIVQVTFSNPGGDVIGIKYKTIDNALEIKNKEDNRGYWDVVWNRPGESNIFDKLQATKFSIIVQNNDRVEISFSKIWSPSMDKTIV PLKVDKRYIVQRGSSGLYLYAIMERLKGWPDVDMDQIRVVFKLQSKKFHYMAISDDRQREMPMPQDRVTGQPLAFPEAVRLTNPVNPKQKGEVDDKYQYS CENKDNKVHGWISNDPPVGFWMITPSNEFRDAGPVKQDLTSHVGPIVLNMFGSVHYAGKDLNAAYRNGEPWKKVFGPVFVYLNSVPSVNPKALWEDAKRQ MSTEVKSWPYNFPRSQDFPSSGQRGNVVGRLVVREQYINKRSMDASFAYVGLAAPGVAGSWQTEAKGYQFWTQADKKGSFSIKNIRAGKYSLYAFVPGFI GDYKYNVDIIIQPGSNIKLGVLTYDPPRNGPTLWEIGIPDRTAAEFYIPDPNPTLINKLYINSPANKFRQYGLWDRYTDLYPKNDLIYTVGVSNYAKDWF YAHVNRRVGNMAYEATTWQIIFELKSAKLSGSYTLQIALASATNSELQVRFNNAKAKRPLFTTRLIGRDNAIARMGIHGLYWLYSIQVPSTQLLQGKNII YLTQSRK | |||
| Full Sequence |
|---|
| Protein Sequence Length: 675 Download |
| MGKMHLLVGS SWPAIVLCFF FFFFIASSAN IPARKVPSRT NNNPALGVQL QVTDKQVVVD 60 NGIVQVTFSN PGGDVIGIKY KTIDNALEIK NKEDNRGYWD VVWNRPGESN IFDKLQATKF 120 SIIVQNNDRV EISFSKIWSP SMDKTIVPLK VDKRYIVQRG SSGLYLYAIM ERLKGWPDVD 180 MDQIRVVFKL QSKKFHYMAI SDDRQREMPM PQDRVTGQPL AFPEAVRLTN PVNPKQKGEV 240 DDKYQYSCEN KDNKVHGWIS NDPPVGFWMI TPSNEFRDAG PVKQDLTSHV GPIVLNMFGS 300 VHYAGKDLNA AYRNGEPWKK VFGPVFVYLN SVPSVNPKAL WEDAKRQMST EVKSWPYNFP 360 RSQDFPSSGQ RGNVVGRLVV REQYINKRSM DASFAYVGLA APGVAGSWQT EAKGYQFWTQ 420 ADKKGSFSIK NIRAGKYSLY AFVPGFIGDY KYNVDIIIQP GSNIKLGVLT YDPPRNGPTL 480 WEIGIPDRTA AEFYIPDPNP TLINKLYINS PANKFRQYGL WDRYTDLYPK NDLIYTVGVS 540 NYAKDWFYAH VNRRVGNMAY EATTWQIIFE LKSAKLSGSY TLQIALASAT NSELQVRFNN 600 AKAKRPLFTT RLIGRDNAIA RMGIHGLYWL YSIQVPSTQL LQGKNIIYLT QSRKGSPFTG 660 IMYDYIRLEA PIKA* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd10316 | RGL4_M | 1.0e-28 | 370 | 469 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 1.0e-50 | 481 | 669 | 191 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 7.0e-76 | 50 | 331 | 288 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 1.0e-85 | 36 | 240 | 207 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_179847.1 | 0 | 42 | 674 | 46 | 677 | lyase [Arabidopsis thaliana] |
| RefSeq | XP_002308510.1 | 0 | 1 | 671 | 1 | 671 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002319997.1 | 0 | 57 | 671 | 1 | 617 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527229.1 | 0 | 1 | 671 | 1 | 667 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527245.1 | 0 | 57 | 674 | 2 | 621 | lyase, putative [Ricinus communis] |
