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Basic Information | |
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Species | Manihot esculenta |
Cazyme ID | cassava4.1_032042m |
Family | CBM57 |
Protein Properties | Length: 992 Molecular Weight: 112057 Isoelectric Point: 7.3069 |
Chromosome | Chromosome/Scaffold: 03542 Start: 244741 End: 249516 |
Description | Di-glucose binding protein with Kinesin motor domain |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM57 | 150 | 278 | 3.8e-23 |
EFLEDTCFAGGDTVRTNAPIGDGQEDGLCLYQTARFGNFSYFFPALEPGNYAVSLHLAEIVFTDGPPRMRMFDALIQEKKKVVSCLDIYAQVGANKPLVI SDLKICIYCDEGLSIRFEGVMGSPIVCGI |
Full Sequence |
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Protein Sequence Length: 992 Download |
MADPDSSANP DTDDFIVVDA SCETTQSLET YDAQKLIRSV ANDAIDDLSM LGFSETSPDL 60 VICSGSPEIP RTTYGDFPEL WDRNKYKGSF ELSLENDIEA SDKKDAHKSL SVKFSSICQT 120 FDKELSPESS LKLLIKPEKE EKFPENPLLE FLEDTCFAGG DTVRTNAPIG DGQEDGLCLY 180 QTARFGNFSY FFPALEPGNY AVSLHLAEIV FTDGPPRMRM FDALIQEKKK VVSCLDIYAQ 240 VGANKPLVIS DLKICIYCDE GLSIRFEGVM GSPIVCGISI TKDSFAGKFN SAPIFWFRLS 300 QEVKEDYREL LRQVEFQKRE LIEMRRALEE LKRENQLKSR ECQDAWKSLQ ELQNELMRKS 360 MHVGSLGKES SLFLCRWFSS LRDMTRKLKF LKFLFQLMKM DHIKLSAEAF AYKKCLADMN 420 ELRLTIQSKM KEQIDLNEDL KIKFTEGAKE RKELYNKVLE LKGNIKVFFR CRPLNAEELA 480 LGASMAIDFE YAEDGELTVT SNGLYRKTFK FDAVFSPQSD QAEVFEDVAP FATSVLDGYN 540 VCIFAYGQTG TGKTFTMEGT EEPRGVNCRI LDEIFHIIKE QNNIFRSLSA LGDVISTLAT 600 KSPHIPFRNS KLTHLLQDSL GGDSKTLMFV QISPNENDLG ETLCSLNFGS RVRGIELGPA 660 QRLLDTAELL RYKQMAKDIQ LKKMEETIHG LELKMKEKDF RNKNLQEKVK ELESQLLIER 720 KLARQHVDSR IAEQQQQMNP QKDEQHSAAP RRPLANRLLG SNKNSSEHKS TEMGKDQVNS 780 SQPLAENNGY TPCIPVHLTE GIARNIDPTE KENKPNMHEQ LGLPEISVRA SICTTSQRIP 840 VAPAPVPRRC SLIPLPSMAG LAGLPPPPPL QPLSLCNDMK EDIEGSEANY LPEQTHCNSP 900 KRIKHRTRKL SSILRRSLKR KIQMKSPAQQ HLRKGGINVG MEKVRISIGS RGIISHRVLL 960 GNGRRPGMKE TQQNRSQREK ERGWNIGKNS T* 1020 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
smart00129 | KISc | 6.0e-31 | 579 | 660 | 84 | + Kinesin motor, catalytic domain. ATPase. Microtubule-dependent molecular motors that play important roles in intracellular transport of organelles and in cell division. | ||
pfam00225 | Kinesin | 2.0e-34 | 470 | 583 | 119 | + Kinesin motor domain. | ||
smart00129 | KISc | 1.0e-37 | 464 | 583 | 123 | + Kinesin motor, catalytic domain. ATPase. Microtubule-dependent molecular motors that play important roles in intracellular transport of organelles and in cell division. | ||
cd01366 | KISc_C_terminal | 8.0e-42 | 579 | 658 | 80 | + Kinesin motor domain, KIFC2/KIFC3/ncd-like carboxy-terminal kinesins. Ncd is a spindle motor protein necessary for chromosome segregation in meiosis. KIFC2/KIFC3-like kinesins have been implicated in motility of the Golgi apparatus as well as dentritic and axonal transport in neurons. This catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Kinesins are microtubule-dependent molecular motors that play important roles in intracellular transport and in cell division. In this subgroup the motor domain is found at the C-terminus (C-type). C-type kinesins are (-) end-directed motors, i.e. they transport cargo towards the (-) end of the microtubule. Kinesin motor domains hydrolyze ATP at a rate of about 80 per second, and move along the microtubule at a speed of about 6400 Angstroms per second. To achieve that, kinesin head groups work in pairs. Upon replacing ADP with ATP, a kinesin motor domain increases its affinity for microtubule binding and locks in place. Also, the neck linker binds to the motor domain, which repositions the other head domain through the coiled-coil domain close to a second tubulin dimer, about 80 Angstroms along the microtubule. Meanwhile, ATP hydrolysis takes place, and when the second head domain binds to the microtubule, the first domain again replaces ADP with ATP, triggering a conformational change that pulls the first domain forward. | ||
cd01366 | KISc_C_terminal | 1.0e-45 | 462 | 580 | 119 | + Kinesin motor domain, KIFC2/KIFC3/ncd-like carboxy-terminal kinesins. Ncd is a spindle motor protein necessary for chromosome segregation in meiosis. KIFC2/KIFC3-like kinesins have been implicated in motility of the Golgi apparatus as well as dentritic and axonal transport in neurons. This catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Kinesins are microtubule-dependent molecular motors that play important roles in intracellular transport and in cell division. In this subgroup the motor domain is found at the C-terminus (C-type). C-type kinesins are (-) end-directed motors, i.e. they transport cargo towards the (-) end of the microtubule. Kinesin motor domains hydrolyze ATP at a rate of about 80 per second, and move along the microtubule at a speed of about 6400 Angstroms per second. To achieve that, kinesin head groups work in pairs. Upon replacing ADP with ATP, a kinesin motor domain increases its affinity for microtubule binding and locks in place. Also, the neck linker binds to the motor domain, which repositions the other head domain through the coiled-coil domain close to a second tubulin dimer, about 80 Angstroms along the microtubule. Meanwhile, ATP hydrolysis takes place, and when the second head domain binds to the microtubule, the first domain again replaces ADP with ATP, triggering a conformational change that pulls the first domain forward. |
Gene Ontology | |
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GO Term | Description |
GO:0003777 | microtubule motor activity |
GO:0005524 | ATP binding |
GO:0007018 | microtubule-based movement |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_002300478.1 | 0 | 49 | 586 | 7 | 551 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317602.1 | 0 | 23 | 586 | 4 | 591 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317602.1 | 0 | 578 | 987 | 718 | 1122 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532828.1 | 0 | 1 | 586 | 1 | 595 | ATP binding protein, putative [Ricinus communis] |
RefSeq | XP_002532828.1 | 0 | 578 | 987 | 722 | 1140 | ATP binding protein, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2ncd_A | 9e-29 | 391 | 584 | 2 | 191 | A Chain A, Ncd (Non-Claret Disjunctional) Dimer From D. Melanogaster |
PDB | 2ncd_A | 2e-16 | 578 | 662 | 313 | 397 | A Chain A, Ncd (Non-Claret Disjunctional) Dimer From D. Melanogaster |
PDB | 1n6m_B | 1e-28 | 438 | 584 | 31 | 180 | A Chain A, Rotation Of The StalkNECK AND ONE HEAD IN A NEW CRYSTAL Structure Of The Kinesin Motor Protein, Ncd |
PDB | 1n6m_B | 3e-16 | 578 | 662 | 302 | 386 | A Chain A, Rotation Of The StalkNECK AND ONE HEAD IN A NEW CRYSTAL Structure Of The Kinesin Motor Protein, Ncd |
PDB | 1n6m_A | 1e-28 | 438 | 584 | 31 | 180 | A Chain A, Rotation Of The StalkNECK AND ONE HEAD IN A NEW CRYSTAL Structure Of The Kinesin Motor Protein, Ncd |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FP030492 | 265 | 578 | 833 | 0 |
FD761463 | 250 | 320 | 559 | 0 |
GW337702 | 222 | 578 | 790 | 0 |
FQ433065 | 205 | 578 | 767 | 0 |
BM404361 | 192 | 578 | 758 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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