| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_033739m |
| Family | GT8 |
| Protein Properties | Length: 470 Molecular Weight: 54269.4 Isoelectric Point: 8.6324 |
| Chromosome | Chromosome/Scaffold: 03614 Start: 3811317 End: 3813099 |
| Description | plant glycogenin-like starch initiation protein 4 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 194 | 387 | 4.79945e-42 |
| EAYVCGAIALAQSIIQTKSTKDLVLLHDSSITPKSLRGLRASGWKTKLIEPIRNPSAKKGSYNEWNYSKLRVWQLSDYDKVIFIDSDIIVLKNLDSFFLY PQISASANDKMLFNSGIMVIEPSKCRLKDLMSKTSKLASYNGGDQGFLNEAFTWWHRLPAKLNHLKVFVRKGNPNHEIPDDVYAVHFLGWKPWM | |||
| Full Sequence |
|---|
| Protein Sequence Length: 470 Download |
| MVDTQKLKIG LVNVDDDEIK SVYYDMYDHG SLAETVSVDF EPVSKDKKWE DFFPEWIDED 60 HKWGSPRCPE IPMPRLEDYL GLDVIVARVP CGGEGKNMKG IRDVWRLQVN LVVANLAVAN 120 GWPVQNQASE DMDRTVYVVF MGSCGPMQEI FRCDDLLTHV GDYWIYKPQL RKLKRKVLMP 180 AGSCQIAPPF AETEAYVCGA IALAQSIIQT KSTKDLVLLH DSSITPKSLR GLRASGWKTK 240 LIEPIRNPSA KKGSYNEWNY SKLRVWQLSD YDKVIFIDSD IIVLKNLDSF FLYPQISASA 300 NDKMLFNSGI MVIEPSKCRL KDLMSKTSKL ASYNGGDQGF LNEAFTWWHR LPAKLNHLKV 360 FVRKGNPNHE IPDDVYAVHF LGWKPWMCYR DYDCNWDIVA RHNFASDSAH RRWWRVYDAM 420 PKKLQKFCGL TRQMDERIRK WRRIAKDQGL PDGHWNINAT DPRRHRLIS* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG5597 | COG5597 | 3.0e-10 | 254 | 304 | 51 | + Alpha-N-acetylglucosamine transferase [Cell envelope biogenesis, outer membrane] | ||
| PLN00176 | PLN00176 | 3.0e-11 | 196 | 418 | 275 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 6.0e-15 | 190 | 346 | 167 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 6.0e-35 | 190 | 386 | 241 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 9.0e-64 | 190 | 420 | 241 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABR67414.1 | 0 | 5 | 468 | 106 | 609 | glycosyl transferase [Cucumis melo] |
| EMBL | CAN81983.1 | 0 | 2 | 468 | 56 | 544 | hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002280832.1 | 0 | 2 | 468 | 56 | 544 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002319544.1 | 0 | 73 | 467 | 1 | 423 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002328440.1 | 0 | 73 | 468 | 1 | 429 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1zcv_A | 4e-17 | 178 | 432 | 14 | 288 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2x_B | 6e-17 | 194 | 386 | 14 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2x_A | 6e-17 | 194 | 386 | 14 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2v_B | 6e-17 | 194 | 386 | 14 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2v_A | 6e-17 | 194 | 386 | 14 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EL376918 | 272 | 193 | 463 | 0 |
| EL450640 | 308 | 133 | 420 | 0 |
| DN503302 | 226 | 187 | 412 | 0 |
| EE614675 | 259 | 133 | 371 | 0 |
| EL445122 | 307 | 44 | 330 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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