| Basic Information | |
|---|---|
| Species | Manihot esculenta |
| Cazyme ID | cassava4.1_034158m |
| Family | GT8 |
| Protein Properties | Length: 585 Molecular Weight: 68906.5 Isoelectric Point: 8.385 |
| Chromosome | Chromosome/Scaffold: 02080 Start: 25164 End: 27509 |
| Description | plant glycogenin-like starch initiation protein 3 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 281 | 502 | 7.00649e-44 |
| IQSTTRTAKREAYVTVLHSSESYVCGAIALAQSLRQTGTKRDLIILLDRSISQPKRDALAAAGWQIRLIKRIRNPRAEKDSYNEYNYSKFRLWQLTDYDK IIFIDSDIIVLRNLDILFHFPQMTAIGNDVWIFNSGIMVIEPSNCTFNFLMDRRSDIISYNGGDQGFLNEVFVWWHRLPRRVNFLKNFWANTTLEASVKN ELFGADPPKVYSIHYLGLKPWN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 585 Download |
| MTEGLSLQKM MKTTPSKALV IRINLVFLAF FLVIYASLLL RPSSSVYFQN AASLVRCSLR 60 ECHHKMKTVL EENQATKQKL KGNMTKIEVP SFMNNMGKGM KIGMVNMDED NVSDWKIHGE 120 TIPIYFERVS EYFKWEDLFP EWIDEEEELE GTSCPEIPMP DFKAYDDMDI IVAKLPCKYP 180 QELWNREVFR LQVHLIAANL AVKKGRRDWS WKTKVVFWSK CRPMLELFRC NDLVRQEGDW 240 WFYEPEMSWL EQKVSLPIGS CKLALPLWAK GIDEIYDMSK IQSTTRTAKR EAYVTVLHSS 300 ESYVCGAIAL AQSLRQTGTK RDLIILLDRS ISQPKRDALA AAGWQIRLIK RIRNPRAEKD 360 SYNEYNYSKF RLWQLTDYDK IIFIDSDIIV LRNLDILFHF PQMTAIGNDV WIFNSGIMVI 420 EPSNCTFNFL MDRRSDIISY NGGDQGFLNE VFVWWHRLPR RVNFLKNFWA NTTLEASVKN 480 ELFGADPPKV YSIHYLGLKP WNCYRDYDCN WNIGDQRVYA SDVAHKRWWK FHDGMDENLQ 540 KFCGLTKQRK IELEWERKMA REMGFSDEHW KIYITDSRRK HLIN* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00505 | Glyco_transf_8 | 2.0e-11 | 292 | 502 | 254 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| PLN00176 | PLN00176 | 9.0e-15 | 282 | 452 | 211 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 8.0e-20 | 292 | 432 | 150 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 5.0e-40 | 294 | 504 | 253 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 4.0e-75 | 291 | 535 | 255 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_001154284.1 | 0 | 14 | 584 | 11 | 594 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_195059.3 | 0 | 14 | 580 | 11 | 590 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002282762.1 | 0 | 3 | 579 | 4 | 588 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002326938.1 | 0 | 224 | 583 | 1 | 360 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002510223.1 | 0 | 1 | 583 | 1 | 589 | glycogenin, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2w_B | 3e-24 | 291 | 506 | 5 | 226 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3u2w_A | 3e-24 | 291 | 506 | 5 | 226 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3qvb_A | 3e-24 | 291 | 506 | 5 | 226 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3q4s_A | 3e-24 | 291 | 506 | 5 | 226 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3u2x_B | 5e-24 | 291 | 506 | 5 | 226 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
