Basic Information | |
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Species | Carica papaya |
Cazyme ID | evm.model.supercontig_124.34 |
Family | GT20 |
Protein Properties | Length: 654 Molecular Weight: 73918.1 Isoelectric Point: 7.7782 |
Chromosome | Chromosome/Scaffold: 124 Start: 588891 End: 590852 |
Description | trehalose-phosphatase/synthase 9 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 41 | 530 | 0 |
SRKKRIIVANFLPLNSRKDSKSGKWFFSLDQDSLLLQMRDGFLSDVDVVYVGSLKVDVKASEQDEVSQTLLEEFNCVPTFLPPDLQKKFYHGFCKQYLWP LFHYMLPICPDYGNCFNRSSWQAYVSANKIFADKVMEVINPEEDFVWVHDYHLMVLPTFLRKHFNQVKLGFFLHSPFPSSEIYRTLPVRDEVLKALLNAD LIGFHTFDYARHFLSCCSRMLGLNYESKRGHIGLEYFGRTVYIKICPVGIHMGQIESALNHPSSSAKVKEIKEKFRGKKIILGVDDMDIFKGISLKLLAM ELLLQQHPEFRGQLVLVQIINPARSTGKDVQEARKETYEITKRINDNFGLLGYEPAVLVDRPVSLHEKTAYYALAECCIVNAVRDGMNLVPYNYIVSRQG TPTVDEALGIALGSPHTSTLVVSEFIGCSPSLSGAIRVNPWDIDAVAEALDVAISMSDVEKQLRHEKHYRYVSSHDVAYWARSFMQDLER |
Full Sequence |
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Protein Sequence Length: 654 Download |
MSDFHQTPGT LPRVMTFPGF ISDVHGDGSN IGDSHSPLTE SRKKRIIVAN FLPLNSRKDS 60 KSGKWFFSLD QDSLLLQMRD GFLSDVDVVY VGSLKVDVKA SEQDEVSQTL LEEFNCVPTF 120 LPPDLQKKFY HGFCKQYLWP LFHYMLPICP DYGNCFNRSS WQAYVSANKI FADKVMEVIN 180 PEEDFVWVHD YHLMVLPTFL RKHFNQVKLG FFLHSPFPSS EIYRTLPVRD EVLKALLNAD 240 LIGFHTFDYA RHFLSCCSRM LGLNYESKRG HIGLEYFGRT VYIKICPVGI HMGQIESALN 300 HPSSSAKVKE IKEKFRGKKI ILGVDDMDIF KGISLKLLAM ELLLQQHPEF RGQLVLVQII 360 NPARSTGKDV QEARKETYEI TKRINDNFGL LGYEPAVLVD RPVSLHEKTA YYALAECCIV 420 NAVRDGMNLV PYNYIVSRQG TPTVDEALGI ALGSPHTSTL VVSEFIGCSP SLSGAIRVNP 480 WDIDAVAEAL DVAISMSDVE KQLRHEKHYR YVSSHDVAYW ARSFMQDLER ACKDHYSKCC 540 CSIGFGLNFR ILSLSPSFKK LPLNHMVSIY KRTCRRAIFL DYDGTLVPQT SIVKAPSPEV 600 ISVLNNLCSD PKNTVFIVSG RGCKSLSDWF TQCGNLGIAA EHGYFIRYVL GTR* 660 |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
TIGR02400 | trehalose_OtsA | 7.0e-137 | 81 | 530 | 451 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. |
PRK14501 | PRK14501 | 9.0e-149 | 43 | 647 | 614 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional |
PLN02205 | PLN02205 | 0 | 11 | 647 | 642 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] |
pfam00982 | Glyco_transf_20 | 0 | 43 | 530 | 492 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. |
cd03788 | GT1_TPS | 0 | 44 | 529 | 486 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_002283215.1 | 0 | 3 | 648 | 20 | 665 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002318643.1 | 0 | 1 | 648 | 17 | 661 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002322159.1 | 0 | 1 | 648 | 17 | 665 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002511428.1 | 0 | 1 | 649 | 17 | 665 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
RefSeq | XP_002521023.1 | 0 | 3 | 648 | 19 | 664 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1gz5_D | 0 | 128 | 530 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_C | 0 | 128 | 530 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_B | 0 | 128 | 530 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_A | 0 | 128 | 530 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 2wtx_D | 0 | 128 | 530 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO777775 | 587 | 65 | 647 | 0 |
HO779327 | 514 | 84 | 597 | 0 |
HO805547 | 281 | 3 | 283 | 0 |
HO805547 | 104 | 285 | 388 | 0 |
HO779327 | 55 | 594 | 648 | 0.000000000000001 |
Sequence Alignments (This image is cropped. Click for full image.) |
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