Basic Information | |
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Species | Carica papaya |
Cazyme ID | evm.model.supercontig_25.167 |
Family | GT48 |
Protein Properties | Length: 979 Molecular Weight: 112272 Isoelectric Point: 9.3588 |
Chromosome | Chromosome/Scaffold: 25 Start: 1670624 End: 1682453 |
Description | glucan synthase-like 12 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT48 | 319 | 815 | 0 |
GPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEALKMRNLLQEFLKKHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRLLANPLR VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNHISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRML SCYFTTVGFYFSTLITVLTVYVFLYGRLYLVLSGLEEGLSKERAIRDNKPLQVALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFVLMQLQLAPVFF TFSLGTKTHYYGRTLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHFVKGIEMMILLIVCQIFGQPYRSAVAYILITISMWFMVGTWLFAPFLFNPSGF EWQKIVDDWTDWNKWINNIGGIGVPPEKSWESWWEEEQEHLRFSGKRGIIVEILLALRFFIYQYGLVYHLTITKRTRSFLVYGISWLVIFLILFVMK |
Full Sequence |
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Protein Sequence Length: 979 Download |
MIDKIISWNI RGLNDHKKRH IIRGCLNKWK PTIICLQEAK IEIITDKTID SIWRMVDVDW 60 KFLPAKGAAG GILIIWRKDK AQCLDVISGE TSISCLFSNN DNGSWYFSEV YCRGNPTKRS 120 LLEEELTHVK NSWREKGIVG GGFNMVLKSS ERSVSSCSIN DIDEFRALID KLDLNDLPLK 180 GRRWTWSNQR SHHSSSRIDR FLISSKLLLE LSSYQEQELW FEFVKKCHQH ECTEKFMDRF 240 PLIYALAINK SATVREAAGF DGYVSWNIPI ARNLNYWELE HVGHGSNTTP LLWHVQHCCG 300 MSNTVVAAIL QISNTTDHGP AILGEGKPEN QNHAIIFTRG EGLQTIDMNQ DNYMEEALKM 360 RNLLQEFLKK HDGVRYPTIL GLREHIFTGS VSSLAWFMSN QENSFVTIGQ RLLANPLRVR 420 FHYGHPDVFD RLFHLTRGGV SKASKVINLS EDIFAGFNST LREGNVTHHE YIQVGKGRDV 480 GLNHISMFEA KIANGNGEQT LSRDIYRLGH RFDFFRMLSC YFTTVGFYFS TLITVLTVYV 540 FLYGRLYLVL SGLEEGLSKE RAIRDNKPLQ VALASQSFVQ IGFLMALPML MEIGLERGFR 600 TALSEFVLMQ LQLAPVFFTF SLGTKTHYYG RTLLHGGAKY RPTGRGFVVF HAKFADNYRL 660 YSRSHFVKGI EMMILLIVCQ IFGQPYRSAV AYILITISMW FMVGTWLFAP FLFNPSGFEW 720 QKIVDDWTDW NKWINNIGGI GVPPEKSWES WWEEEQEHLR FSGKRGIIVE ILLALRFFIY 780 QYGLVYHLTI TKRTRSFLVY GISWLVIFLI LFVMKTVSVG RRKFSANFQL MFRLIKGLIF 840 MTFVAILVTL IALPHMTVQD IIVCILAFMP TGWGMLLIAQ ACKPVVQRAG FWGSVGTLAR 900 GYEIVMGLLL FTPVAFLAWF PFVSEFQTRM LFNQAFSRGL QISRILDSSI SLIQLHQGAL 960 RSVKNSVHIY LYARCCLC* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd08372 | EEP | 0.003 | 5 | 100 | 99 | + Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily. This large superfamily includes the catalytic domain (exonuclease/endonuclease/phosphatase or EEP domain) of a diverse set of proteins including the ExoIII family of apurinic/apyrimidinic (AP) endonucleases, inositol polyphosphate 5-phosphatases (INPP5), neutral sphingomyelinases (nSMases), deadenylases (such as the vertebrate circadian-clock regulated nocturnin), bacterial cytolethal distending toxin B (CdtB), deoxyribonuclease 1 (DNase1), the endonuclease domain of the non-LTR retrotransposon LINE-1, and related domains. These diverse enzymes share a common catalytic mechanism of cleaving phosphodiester bonds; their substrates range from nucleic acids to phospholipids and perhaps proteins. | ||
cd09073 | ExoIII_AP-endo | 0.001 | 4 | 42 | 39 | + Escherichia coli exonuclease III (ExoIII)-like apurinic/apyrimidinic (AP) endonucleases. The ExoIII family AP endonucleases belong to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds. AP endonucleases participate in the DNA base excision repair (BER) pathway. AP sites are one of the most common lesions in cellular DNA. During BER, the damaged DNA is first recognized by DNA glycosylase. AP endonucleases then catalyze the hydrolytic cleavage of the phosphodiester bond 5' to the AP site, which is then followed by the coordinated actions of DNA polymerase, deoxyribose phosphatase, and DNA ligase. If left unrepaired, AP sites block DNA replication, which have both mutagenic and cytotoxic effects. AP endonucleases can carry out a wide range of excision and incision reactions on DNA, including 3'-5' exonuclease, 3'-deoxyribose phosphodiesterase, 3'-phosphatase, and occasionally, nonspecific DNase activities. Different AP endonuclease enzymes catalyze the different reactions with different efficiences. Many organisms have two functional AP endonucleases, for example, APE1/Ref-1 and Ape2 in humans, Apn1 and Apn2 in bakers yeast, Nape and NExo in Neisseria meningitides, and exonuclease III (ExoIII) and endonuclease IV (EndoIV) in Escherichia coli. Usually, one of the two is the dominant AP endonuclease, the other has weak AP endonuclease activity, but exhibits strong 3'-5' exonuclease, 3'-deoxyribose phosphodiesterase, and 3'-phosphatase activities. Class II AP endonucleases have been classified into two families, designated ExoIII and EndoIV, based on their homology to the Escherichia coli enzymes. This family contains the ExoIII family; the EndoIV family belongs to a different superfamily. | ||
TIGR00195 | exoDNase_III | 0.001 | 4 | 42 | 39 | + exodeoxyribonuclease III. The model brings in reverse transcriptases at scores below 50, model also contains eukaryotic apurinic/apyrimidinic endonucleases which group in the same family [DNA metabolism, DNA replication, recombination, and repair]. | ||
cd09076 | L1-EN | 5.0e-11 | 5 | 201 | 220 | + Endonuclease domain (L1-EN) of the non-LTR retrotransposon LINE-1 (L1), and related domains. This family contains the endonuclease domain (L1-EN) of the non-LTR retrotransposon LINE-1 (L1), and related domains, including the endonuclease of Xenopus laevis Tx1. These retrotranspons belong to the subtype 2, L1-clade. LINES can be classified into two subtypes. Subtype 2 has two ORFs: the second (ORF2) encodes a modular protein consisting of an N-terminal apurine/apyrimidine endonuclease domain (EN), a central reverse transcriptase, and a zinc-finger-like domain at the C-terminus. LINE-1/L1 elements (full length and truncated) comprise about 17% of the human genome. This endonuclease nicks the genomic DNA at the consensus target sequence 5'TTTT-AA3' producing a ribose 3'-hydroxyl end as a primer for reverse transcription of associated template RNA. This subgroup also includes the endonuclease of Xenopus laevis Tx1, another member of the L1-clade. This family belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds. | ||
pfam02364 | Glucan_synthase | 3.0e-165 | 319 | 813 | 546 | + 1,3-beta-glucan synthase component. This family consists of various 1,3-beta-glucan synthase components including Gls1, Gls2 and Gls3 from yeast. 1,3-beta-glucan synthase EC:2.4.1.34 also known as callose synthase catalyzes the formation of a beta-1,3-glucan polymer that is a major component of the fungal cell wall. The reaction catalyzed is:- UDP-glucose + {(1,3)-beta-D-glucosyl}(N) <=> UDP + {(1,3)-beta-D-glucosyl}(N+1). |
Gene Ontology | |
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GO Term | Description |
GO:0000148 | 1,3-beta-D-glucan synthase complex |
GO:0003843 | 1,3-beta-D-glucan synthase activity |
GO:0006075 | (1->3)-beta-D-glucan biosynthetic process |
GO:0016020 | membrane |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABN09771.1 | 0 | 319 | 946 | 604 | 1232 | Glycosyl transferase, family 48 [Medicago truncatula] |
RefSeq | NP_196804.5 | 0 | 300 | 946 | 1323 | 1986 | ATGSL12 (glucan synthase-like 12); 1,3-beta-glucan synthase/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_002299147.1 | 0 | 319 | 946 | 1300 | 1927 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002304888.1 | 0 | 319 | 946 | 1319 | 1948 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002528124.1 | 0 | 319 | 946 | 1315 | 1942 | transferase, transferring glycosyl groups, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2v0r_B | 0.007 | 5 | 208 | 10 | 216 | A Chain A, Crystal Structure Of A Hairpin Exchange Variant (Ltx) Of The Targeting Line-1 Retrotransposon Endonuclease |
PDB | 2v0r_A | 0.007 | 5 | 208 | 10 | 216 | A Chain A, Crystal Structure Of A Hairpin Exchange Variant (Ltx) Of The Targeting Line-1 Retrotransposon Endonuclease |
PDB | 1vyb_B | 0.007 | 5 | 208 | 10 | 214 | A Chain A, Endonuclease Domain Of Human Line1 Orf2p |
PDB | 1vyb_A | 0.007 | 5 | 208 | 10 | 214 | A Chain A, Endonuclease Domain Of Human Line1 Orf2p |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
callose biosynthesis | 13-BETA-GLUCAN-SYNTHASE-RXN | EC-2.4.1.34 | 1,3-β-glucan synthase |